HPLC–MS/MS determination of flavonoids in Gleditsiae Spina for its quality assessment

Abstract

Gleditsiae Spina, the thorn of Gleditsia sinensis Lam., has been used as an anti-inflammatory, anti-tumor, and anti-bacterial traditional medicine for hundreds of years in China. This study used high-performance liquid chromatography and tandem mass spectrometry combined with chemometric methods to allow the fast and accurate identification and quantification of the flavonoids compounds in Gleditsiae Spina, and created reliable criteria for accurate identification of Gleditsiae Spina and its adulterants. This research provides good evidence for the classification and quality evaluation of Gleditsiae Spina. Firstly, eight flavonoids compounds were detected and identified on the basis of their mass spectra, fragment characteristics, and comparison with published data. Then the mass spectroscopic fragmentation pathways of these compounds were determined and, in addition rutin, isoquercitrin, and quercitrin were detected in Gleditsiae Spina for the first time. The quantification was performed on a triple quadrupole tandem mass spectrometer in multi-reaction monitoring mode, and the baseline separation of the eight bioactive flavonoids components was achieved within 13 min. Furthermore, the proposed method was successfully applied for simultaneous quantitative determination of the eight Gleditsiae Spina compounds and adulterants obtained from different sources in China. Then, we built a classification model which showed a high level of accuracy predicting 100% of the samples, correctly.

Analysis of the active components and metabolites of Taohong Siwu decoction by using ultra high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

“…A total of 10 flavonoid‐related metabolites were identified based on accurate molecular weight measurements, metabolite fragmentation patterns, and identification of metabolic pathways. Fragmentation of flavonoid aglycones can be divided into two major categories: retro Diels‐Alder (RDA) reaction, which cleaves flavonoids into two parts [21–22]; and loss of small molecule fragments such as CO, CHO, CO 2 , and C 2 H 2 O [23,24]. Figure 2 uses M66 to explain the identification process.…”

HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus

“…One of the TCM preparations, HXJTY, has been commonly used to treat patients with T2DM, but a suitable quality control method is not available to assure the efficacy of HXJTY. Recently, HPLC combined with MS has been widely employed to establish a quality control method by qualitative and quantitative determination of the major constitutes within herbal medicine [10,11]. However, it is still not able to distinguish the quality markers, which represent the efficacy to the greatest extent in the whole prescription, from a large number of chemical markers.…”

The Analyses of Chemical Components From Oldenlandia hedyotidea (DC.) Hand.-Mazz and Anticancer Effects in vitro

“…In this study, we attempted to detect and characterize the major constituents in OH using ultra high performance liquid chromatography–mass spectrometry (UHPLC-MS) ( Li et al, 2003 ; Li J. et al, 2018 ; Siegle and Pietsch, 2018 ) and UHPLC triple time-of-flight mass spectrometry (UHPLC-Triple-TOF-MS) ( Zhao et al, 2017 ; Huo et al, 2018 ), for identifying the major constituents of OH for the first time. By combining three identification techniques, namely, plant DNA barcoding ( Chen et al, 2010 ; Yao et al, 2010 ), thin-layer chromatography (TLC) ( Mustarichie et al, 2019 ; Ansari et al, 2020 ; Jakinala et al, 2021 ), and microscopic identification ( Li et al, 2015 ), we verified the different batches of OH, both authentic and inauthentic, collected from different provinces.…”