HPLC/MS-MS Identification of Oak Quercus aegilops Root Tannins

Amr, Ayed; Ahmad, Mousa Numan; Zahra, Jalal A.; Abdullah, Mai Adnan

doi:10.1155/2021/8882050

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Recent research revealed that HTs' antioxidant activity may lower insulin‐induced lipogenesis, blood triglycerides, and cholesterol 51 . Notably, the amount of HTs depends on the species, region, and age of the plant 52 . Both NRF and NRL are abundant in HT.…”

Section: Resultsmentioning

confidence: 99%

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Biological activities, identification, method development, and validation for analysis of polyphenolic compounds in Nymphaea rubra flowers and leaves by UHPLC‐Q‐cIM‐TOF‐MS and UHPLC‐TQ‐MS

Naznin,

Alam,

Alam

et al. 2024

Phytochemical Analysis

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IntroductionNymphaea rubra belongs to the Nymphaea family and is regarded as a vegetable used in traditional medicine to cure several ailments. These species are rich in phenolic acid, flavonoids, and hydrolysable tannin.ObjectiveThis study aimed to assess the biological activities of Nymphaea rubra flowers (NRF) and leaves (NRL) by identifying and quantifying their polyphenolic compounds using ultra‐performance liquid chromatography coupled to quadrupole cyclic ion mobility time‐of‐flight mass spectrometry (UHPLC‐Q‐cIM‐TOF‐MS) and triple quadrupole mass spectrometry (UHPLC‐TQ‐MS).MethodologyNRF and NRL powder was extracted with methanol and fractionated using hexane, ethylacetate, and water. Antioxidant and α‐glucosidase, and tyrosinase enzyme inhibitory activities were evaluated. The polyphenolic components of NRF and NRL were identified and quantified using UHPLC‐Q‐cIM‐TOF‐MS and UHPLC‐TQ‐MS. The method was validated using linearity, precision, accuracy, limit of detection (LOD), and lower limit of quantification (LLOQ).ResultsBioactive substances and antioxidants were highest in the ethylacetate fraction of flowers and leaves. Principal component analysis showed how solvent and plant components affect N. rubra's bioactivity and bioactive compound extraction. A total of 67 compounds were identified, and among them 21 significant polyphenols were quantified. Each calibration curve had R2 > 0.998. The LOD and LLOQ varied from 0.007 to 0.09 μg/mL and from 0.01 to 0.1 μg/mL, respectively. NRF contained a significant amount of gallic acid (10.1 mg/g), while NRL contained abundant pentagalloylglucose (2.8 mg/g).ConclusionThe developed method is simple, rapid, and selective for the identification and quantification of bioactive molecules. These findings provide a scientific basis for N. rubra's well‐documented biological effects.

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Section: Resultsmentioning

confidence: 99%

“…51 Notably, the amount of HTs depends on the species, region, and age of the plant. 52 Both NRF and NRL are abundant in HT. NRL has 2.06 ± 1.54 and 2.80 ± 1.97 mg/g of CRG and PGG, respectively, compared with NRF's 2.50 ± 1.01 and 1.84 ± 2.73 mg/g.…”

Section: Methods Validationmentioning

confidence: 99%

Biological activities, identification, method development, and validation for analysis of polyphenolic compounds in Nymphaea rubra flowers and leaves by UHPLC‐Q‐cIM‐TOF‐MS and UHPLC‐TQ‐MS

Naznin,

Alam,

Alam

et al. 2024

Phytochemical Analysis

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Green extraction of Quercus infectoria gall with supercritical CO2 and methanol co-solvent

Purbowati

Taufikurohmah

Syahrani

2023

Environ Sci Pollut Res

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A comparative study of phenolic acid extract from Quercus infectoria Gall using supercritical uid extraction of CO 2 (SCFE-CO 2 ) with co-solvent methanol can produce yields, phenolic acid compositions, and differences in the extract's toxicity. SCFE-CO 2 was carried out with the same weight of material (Q. infectoria gall) and run at a temperature of 60 o C and a pressure of 20 MPa with methanol as the cosolvent and an extraction time of 60 minutes. The extraction process extracts were identi ed by yield, phenolic acid composition using LC-MS/MS, total phenolic acid, and toxicity. The results of the SCFE-CO 2 extraction technique with co-solvent methanol do not produce extracts that are toxic but have high yields and tannic acid compound (TPC). The SCFE-CO 2 technique has small extract variability (div. sd. 0.1%), a high yield, a high tannic acid compound, a high TPC, and non-toxic, so SCFE produces extracts that have maintainable quality. The small amount of addition of methanol co-solvent can increase the quality of the extract using the SCFE-CO 2 technique, and increasing the addition of methanol cannot increase yield.

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Effect of pulsed ultrasound assisted extraction and aqueous acetone mixture on total hydrolysable tannins from pomegranate peel

Rifna

Dwivedi

2022

Food Bioscience

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HPLC/MS-MS Identification of Oak Quercus aegilops Root Tannins

Cited by 6 publications

References 28 publications

Biological activities, identification, method development, and validation for analysis of polyphenolic compounds in Nymphaea rubra flowers and leaves by UHPLC‐Q‐cIM‐TOF‐MS and UHPLC‐TQ‐MS

Biological activities, identification, method development, and validation for analysis of polyphenolic compounds in Nymphaea rubra flowers and leaves by UHPLC‐Q‐cIM‐TOF‐MS and UHPLC‐TQ‐MS

Green extraction of Quercus infectoria gall with supercritical CO2 and methanol co-solvent

Effect of pulsed ultrasound assisted extraction and aqueous acetone mixture on total hydrolysable tannins from pomegranate peel

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