HPLC profile and antihyperglycemic effect of ethanol extracts of Andrographis paniculata in normal and streptozotocin-induced diabetic rats

Ahmad, Mariam; Razak, Abdul; Akowuah, Gabriel Akyirem; Asmawi, Zaini; Ismail, Zhari

doi:10.1007/s11418-007-0157-4

Cited by 12 publications

(7 citation statements)

References 5 publications

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“…At the end of the experimental period, blood samples were collected for the measurement of blood glucose level and plasma insulin. An intraperitoneal glucose tolerance test (IPGTT) was performed at the end of 14-day treatment [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Blood samples were collected from the tail vein at time –60 min (just before the administration of the extract by gastric intubation), time 0 (prior to the glucose load) and 15, 30, 45, 60, 90 and 120 min after the glucose load. Blood glucose concentration was measured using the Accu-check Advantage II Clinical Glucose meter (Roche diagnostics Co. USA) [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

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Antidiabetic Properties and Mechanism of Action of Gynura procumbens Water Extract in Streptozotocin-Induced Diabetic Rats

et al. 2010

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Gynura procumbens (Lour.) Merr (family Compositae) is cultivated in Southeast Asia, especially Indonesia, Malaysia and Thailand, for medicinal purposes. This study evaluated the in vivo hypoglycemic properties of the water extract of G. procumbens following 14 days of treatment and in vitro in RIN-5F cells. Glucose absorption from the intestines and its glucose uptake in abdominal skeletal muscle were assessed. The antidiabetic effect of water extract of G. procumbens leaves was investigated in streptozotocin-induced diabetic rats. The intraperitoneal glucose tolerance test (IPGTT) was performed in diabetic rats treated with G. procumbens water extract for 14 days. In the IPGTT, blood was collected for insulin and blood glucose measurement. After the IPGTT, the pancreases were collected for immunohistochemical study of β-cells of the islets of Langerhans. The possible antidiabetic mechanisms of G. procumbens were assessed through in vitro RIN-5F cell study, intestinal glucose absorption and glucose uptake by muscle. The results showed that G. procumbens significantly decreased blood glucose levels after 14 days of treatment and improved outcome of the IPGTT. However, G. procumbens did not show a significant effect on insulin level either in the in vivo test or the in vitro RIN-5F cell culture study. G. procumbens also showed minimal effects on β-cells of the islets of Langerhans in the pancreas. However, G. procumbens only significantly increased glucose uptake by muscle tissues. From the findings we can conclude that G. procumbens water extract exerted its hypoglycemic effect by promoting glucose uptake by muscles.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antidiabetic Properties and Mechanism of Action of Gynura procumbens Water Extract in Streptozotocin-Induced Diabetic Rats

et al. 2010

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“…Meanwhile, a study by Ahmad et al (2007) determined the antihyperglycemic effects of different extraction solvents in normal and streptozotocin-induced diabetic rats. Aerial parts of A. paniculata were extracted with different concentration of ethanol (20%, 50%, and 95%) and distilled water by using reflux techniques at 40 °C for 3 days.…”

Section: Overview Of Studies Regarding Extraction Techniques and The mentioning

confidence: 99%

A review on extraction techniques and therapeutic value of polar bioactives from Asian medicinal herbs: Case study on Orthosiphon aristatus, Eurycoma longifolia and Andrographis paniculata

Aziz

Hasham

Sarmidi

et al. 2021

Saudi Pharmaceutical Journal

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Medicinal plants have gained much interest in the prevention and treatment of common human disease such as cold and fever, hypertension and postpartum. Bioactive compounds from medicinal plants were synthesised using effective extraction methods which have important roles in the pharmaceutical product development. Orthosiphon aristatus (OA), Eurycoma longifolia (EL) and Andrographis paniculata (AP) are among popular medicinal herbs in Southeast Asia. The major compounds for these medicinal plants are polar bioactive compounds (rosmarinic acid, eurycomanone and andrographolide) which have multiple benefits to human health. The bioactive compounds are used as a drug to function against a variety of diseases with the support of scientific evidence. This paper was intended to prepare a complete review about the extraction techniques (e.g. OA, EL and AP) of these medicinal plants based on existing studies and scientific works. Suitable solvents and techniques to obtain their major bioactive compounds and their therapeutic potentials were discussed.

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“…monograph. Several HPLC methods have been reported for the qualitative and quantitative determination of andrographolides [8][9][10]. A detailed evaluation of the literature data showed that the reported HPLC methods suffer from extremely fast run times that may not afford a detailed chromatographic fingerprint or do not include an exhaustive extraction of the raw material.…”

Section: Edqmmentioning

confidence: 99%

A Validated Method for the Quality Control of Andrographis paniculata Preparations

et al. 2017

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is a herbal drug of Asian traditional medicine largely employed for the treatment of several diseases. Recently, it has been introduced in Europe for the prophylactic and symptomatic treatment of common cold and as an ingredient of dietary supplements. The active principles are diterpenes with andrographolide as the main representative. In the present study, an analytical protocol was developed for the determination of the main constituents in the herb and preparations of . Three different extraction protocols (methanol extraction using a modified Soxhlet procedure, maceration under ultrasonication, and decoction) were tested. Ultrasonication achieved the highest content of analytes. HPLC conditions were optimized in terms of solvent mixtures, time course, and temperature. A reversed phase C18 column eluted with a gradient system consisting of acetonitrile and acidified water and including an isocratic step at 30 °C was used. The HPLC method was validated for linearity, limits of quantitation and detection, repeatability, precision, and accuracy. The overall method was validated for precision and accuracy over at least three different concentration levels. Relative standard deviation was less than 1.13%, whereas recovery was between 95.50% and 97.19%. The method also proved to be suitable for the determination of a large number of commercial samples and was proposed to the European Pharmacopoeia for the quality control of Andrographidis herba.

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HPLC profile and antihyperglycemic effect of ethanol extracts of Andrographis paniculata in normal and streptozotocin-induced diabetic rats

Cited by 12 publications

References 5 publications

Antidiabetic Properties and Mechanism of Action of Gynura procumbens Water Extract in Streptozotocin-Induced Diabetic Rats

Antidiabetic Properties and Mechanism of Action of Gynura procumbens Water Extract in Streptozotocin-Induced Diabetic Rats

A review on extraction techniques and therapeutic value of polar bioactives from Asian medicinal herbs: Case study on Orthosiphon aristatus, Eurycoma longifolia and Andrographis paniculata

A Validated Method for the Quality Control of Andrographis paniculata Preparations

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