HPLC separation of pentazocine enantiomers in serum using an ovomucoid chiral stationary phase

Kelly, James W.; Stewart, James T.; Blanton, C. DeWitt

doi:10.1002/bmc.1130080512

Cited by 13 publications

(4 citation statements)

References 18 publications

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“…As will be seen later, various naturally occurring proteins and carbohydrates have been used as ligands for chiral separations of clinical analytes (142)(143)(144)(145)(146)(147)(148)(149)(150)(151)(152)(153)(154)(155)(156)(157)(158)(159)(160)(161). Other, synthetic ligands that have also been used for chiral separations with clinical samples, such as derivatives of amylose or cellulose and Pirkle-type stationary phases , but these other ligands will not be considered in this present review.…”

Section: Affinity-based Chiral Separationsmentioning

confidence: 99%

“…BSA, and the related protein HSA, tend to bind best to neutral or anionic compounds, thus making these proteins complementary to AGP and ovomucoid in their applications (139,141 ). In clinical work, BSA has been used for the chiral separation of leucovorin in plasma (156 ), and ovomucoid has been used for separating the individual forms of pentazocine in serum samples (157 ).…”

Section: Protein-based Stationary Phasesmentioning

confidence: 99%

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Affinity Chromatography: A Review of Clinical Applications

Hage

1999

Clinical Chemistry

229

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Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with other analytical methods, such as reversed-phase liquid chromatography, gas chromatography, and capillary electrophoresis. Indirect analyte detection methods are also described in which immunoaffinity chromatography is used to perform flow-based immunoassays. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins. Some areas of possible future developments are then considered, such as tandem affinity methods and the use of synthetic dyes, immobilized metal ions, molecular imprints, or aptamers as affinity ligands for clinical analytes.

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Section: Affinity-based Chiral Separationsmentioning

confidence: 99%

Section: Protein-based Stationary Phasesmentioning

confidence: 99%

Affinity Chromatography: A Review of Clinical Applications

Hage

1999

Clinical Chemistry

229

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“…The combination of pentazocine and naloxone is used to reduce pain that is extreme requiring opioid therapy as it may not be tolerated or other pain medications have not worked well enough [13,14]. Pentazocine was quantified in pharmaceutical samples [15][16][17], human serum [18], human plasma [19][20][21], human urine [21,22], and whole human blood [22] using visible spectrophotometry [15,16], Thin-layer chromatography [17], high-performance liquid chromatographic (HPLC) [18][19][20], potentiometry [21], and gas chromatography [22]. Naloxone was quantified in microparticles [23], dosage forms [24], transdermal formulations [25], human plasma [26][27][28], human urine [28], and human liver microsomes [28] using spectrophotometry [23], HPLC [23][24][25][26][27], and liquid chromatography-mass spectrometry [28].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Pentazocine and Naloxone by Stability Indicating Reverse-Phase-High-Performance Liquid Chromatographic Method

Kandula¹,

Sundararajan²

2019

Asian J Pharm Clin Res

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Objective: The objective of the study was to develope a stability indicating high-performance liquid chromatographic (HPLC) method for simultaneous assay of pentazocine and naloxone in bulk and tablets. Methods: Pentazocine and naloxone were analyzed on Dionex C18 column using 0.1M K2HPO4 buffer (pH 4.0) and methanol (60:40, v/v) as the mobile phase. The concentration of pentazocine and naloxone was quantified by photodiode array detector set at 248 nm. The method was validated in compliance with ICH rules. Pentazocine and naloxone tablet formulation was subjected to forced degradation such as acid, neutral and alkali hydrolysis, oxidation, photo, and thermal degradation. Results: The method was linear, with R2=0.9999 in the concentration range 100–300 μg/ml for pentazocine and R2=0.9995 in the concentration range 1–3 μg/ml for naloxone. The level of detection and quantification was 0.097 μg/ml and 0.322 μg/ml for pentazocine and 0.0073 μg/ml and 0.0243 μg/ml for naloxone, respectively. The degraded products are resolved well from pentazocine and naloxone with significantly different retention time values. From validation results, it was proved that the method is selective, precise, robust, and accurate for the estimation of pentazocine and naloxone simultaneously. Conclusion: The developed stability-indicating HPLC method can be applied for quantitative determination of pentazocine and naloxone in tablets.

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“…The reported limits of detection (LODs) in HPLC studies with different detection methods, such as ultraviolet (UV) (10), fluorescence (FL) (11)(12)(13) and LC-mass spectroscopy (MS) (14) were in the range 0.1 ng/g tissue (14)-15 ng/mL in serum (10). While GC-MS showed 0.5 ng/mL LOD, it used 1 mL blood or urine.…”

mentioning

confidence: 95%

Pentazocine monitoring in rat hair and plasma by HPLC‐fluorescence detection with DIB‐Cl as a labelling reagent

et al. 2006

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Pentazocine (PZ) in rat hair and plasma was determined by HPLC-fluorescence detection with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a labelling reagent and cyclazocine (CZ) as an internal standard (IS). PZ and IS extracted from hair or plasma sample were derivatized with DIB-Cl and the resulted solution was cleaned up with solid phase extraction. The isocratic separation of DIB-PZ and -CZ within 20 min could be achieved by a Wakopak Handy-ODS column (250 x 4.6 mm i.d.) using a mobile phase composed of 0.1 mol/L acetate buffer (pH 6.2):acetonitrile (25:75, v/v). The detection limits of PZ at a signal-to-noise ratio of 3 for rat hair and plasma were 0.18 ng/mg and 0.57 ng/mL, respectively. Reproducible and precise results could be obtained by an IS method with RSD values less than 6.6% for within- and between-day measurements. The method was successfully applied for the monitoring of PZ levels in Zucker rat hair and plasma samples after a single administration of 25 mg/kg PZ. Moreover, incorporation rates of PZ into black and white hair of Zucker rat were evaluated.

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HPLC separation of pentazocine enantiomers in serum using an ovomucoid chiral stationary phase

Cited by 13 publications

References 18 publications

Affinity Chromatography: A Review of Clinical Applications

Affinity Chromatography: A Review of Clinical Applications

Simultaneous Quantification of Pentazocine and Naloxone by Stability Indicating Reverse-Phase-High-Performance Liquid Chromatographic Method

Pentazocine monitoring in rat hair and plasma by HPLC‐fluorescence detection with DIB‐Cl as a labelling reagent

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