Pentazocine monitoring in rat hair and plasma by HPLC‐fluorescence detection with DIB‐Cl as a labelling reagent

Abstract

Pentazocine (PZ) in rat hair and plasma was determined by HPLC-fluorescence detection with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a labelling reagent and cyclazocine (CZ) as an internal standard (IS). PZ and IS extracted from hair or plasma sample were derivatized with DIB-Cl and the resulted solution was cleaned up with solid phase extraction. The isocratic separation of DIB-PZ and -CZ within 20 min could be achieved by a Wakopak Handy-ODS column (250 x 4.6 mm i.d.) using a mobile phase composed of 0.1 mol/L acetate buffer (pH 6.2):acetonitrile (25:75, v/v). The detection limits of PZ at a signal-to-noise ratio of 3 for rat hair and plasma were 0.18 ng/mg and 0.57 ng/mL, respectively. Reproducible and precise results could be obtained by an IS method with RSD values less than 6.6% for within- and between-day measurements. The method was successfully applied for the monitoring of PZ levels in Zucker rat hair and plasma samples after a single administration of 25 mg/kg PZ. Moreover, incorporation rates of PZ into black and white hair of Zucker rat were evaluated.

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

“…Additionally, HPLC-FL determination of Mor 58 and pentazocine (PZ) 59 labeled with DIB-Cl were examined. Both medicaments are used as an analgesic for post-operative or cancer pain and are also illegally used as drugs.…”

Rapid Determination of Pentazocine in Human Plasma and Urine by a Potentiometric Method

“…Accurate and simple methods for analysis of the drug in human samples are been required in the fields of clinical and forensic toxicology. Various methods cited in literature for its determinations involve high-performance liquid chromatography (HPLC) (Wilson 1982), HPLC=mass spectrometry (MS) (Arinobu et al 2003;Imamura et al 1999), gas chromatography (GC)=MS (Chan, Hsu, and Chu 2007), linear sweep voltammetry (Chann and Fogg 1979), spectrophotometry (Sastry and Rao 1987;Lavanya and Baggi 1989;Chatterjee, Jain, and Sethi 1987), and HPLC=fluorimetry using postcolumn reaction (Wada et al 2007). However, most of these methods involve time-consuming procedures (Wilson 1982;Arinobu et al 2003;Imamura et al 1999;Chan, Hsu, and Chu 2007;Wada et al 2007), involve derivatization (Wada et al 2007), have low sensitivity (Chann and Fogg 1979;Sastry and Rao 1987;Lavanya and Baggi 1989;Chatterjee, Jain, and Sethi 1987), and=or use sophisticated and expensive instruments (Arinobu et al 2003;Imamura et al 1999;Chan, Hsu, and Chu 2007).…”

Quantification of three triterpenic acids in dried rosemary using HPLC‐fluorescence detection and 4‐(4,5‐diphenyl‐1H‐imidazole‐2‐yl)benzoyl chloride derivatization

“…In conventional methods, derivatization reagents reacted with carboxyl group in triterpenic acids were used, but they needed time‐consuming reaction conditions . Conversely, 4‐(4,5‐diphenyl‐1 H ‐imidazole‐2‐yl)benzoyl chloride (DIB‐Cl), which reacted with phenolic compounds rapidly in our previous studies, may be available as a derivatization reagent for triterpenic acids.…”