HPLC simultaneous determination of arbutin, chlorogenic acid and 6′-O-caffeoylarbutin in different parts ofVaccinium dunalianumWight

Abstract

Arbutin (1), chlorogenic acid (2) and 6'-O-caffeoylarbutin (3) are three major components in Vaccinium dunalianum Wight with various promising bioactivities. A reliable, reproducible and accurate method for simultaneous and quantitative determination of 1-3 is developed by RP-HPLC analysis. This method should be appropriate for the quality assurance of unprocessed and processed materials of V. dunalianum. The contents of 1-3 in different parts of V. dunalianum from different origins were analysed. The content of 3 was much higher than those of 1 and 2, accounting for up to 31.76% in the dried leaf buds. Moreover, the leaf buds, flower buds and leaves showed a tendency towards higher contents of 1-3 than the other plant parts.

Caffeic acid derivatives (CAFDs) as inhibitors of SARS-CoV-2: CAFDs-based functional foods as a potential alternative approach to combat COVID-19

“…6′-O-caffeoylarbutin is abundantly found in Vaccinium dunalianumas. V. dunalianumas is a commonly cultivated blueberry species in China and its dried leaf buds as herbal tea while its leaves are used as folk medicine ( Li et al., 2016 ; Luo et al., 2015 ). Sunflower ( Helianthus annuus ) sprouts (nutritious sunflower lettuce) found to be enriched with cynarin ( Sun et al., 2012 ), recommending these foods as beneficial food choice for COVID-19 patients.…”

Diversity of endophytic fungi from the leaves of Vaccinium dunalianum

“…Vaccinium dunalianum Wight is an evergreen, small shrub tree species that is mainly distributed in Sichuan Province, Yunnan Province, Guizhou Province and Tibet. Its leaves have been used as folk medicine for treating articular rheumatism, relaxing tendons, and activating collaterals, and dried leaf buds are used as herbal tea by local people in China (Luo et al 2015). Previous studies found that the leaf buds of V. dunalianum Wight are rich in arbutin, 6 0 -O-caffeoylarbutin and chlorogenic acid (Zhao et al 2008), which exhibit antioxidant, depigmentation (Xu et al 2014) and DNA-protective effects (Jang et al 2014).…”

“…Its leaves have been used as folk medicine for treating articular rheumatism, relaxing tendons, and activating collaterals, and dried leaf buds are used as herbal tea by local people in China (Luo et al . 2015). Previous studies found that the leaf buds of V. dunalianum Wight are rich in arbutin, 6′‐ O ‐caffeoylarbutin and chlorogenic acid (Zhao et al .…”

Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies

“…In China, V. dunalianum Wight leaves are used as a traditional treatment for articular rheumatism. Additionally, local people use the dried buds of V. dunalianum Wight as a substituted for tea (Luo et al 2015b). Previous studies found that the leaf buds of V.…”

“…dunalianum Wight is rich in the natural whitening active agent arbutin (Li et al 2017;Luo et al 2015a). However, research on gene expression and molecular biology of V.…”