HPLC–UV method validation for the identification and quantification of bioactive amines in commercial eggs

Abstract

A quantitative and confirmatory high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method for the determination of bioactive amines in the albumen and yolk of commercial eggs was developed, optimized and validated by analyte extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine standards were used to evaluate the following performance parameters: limit of detection (LoD), limit of quantification (LoQ), selectivity, linearity, precision, recovery and ruggedness. The LoD of the method was defined from 0.2 to 0.3 mg kg(-1) for the yolk matrix and from 0.2 to 0.4 mg kg(-1) for the albumen matrix; the LoQ was from 0.7 to 1.0 mg kg(-1) for the yolk matrix and from 0.7 to 1.1 mg kg(-1) for the albumen matrix. The validated method exhibited excellent selectivity and separation of all amines with coefficients of determination higher than 0.99. The obtained recovery values were from 90.5% to 108.3%, and the relative standard deviation (RSD) was lower than 10% under repeatability conditions for the studied analytes. The performance parameters show the validated method to be adequate for the determination of bioactive amines in egg albumen and yolk.

Cellulose‐Based Substrate for SERS‐Promoted Histamine Picomolar Detection in Beverages

“…The effective concentration of histamine in the two wine samples was measured by means of standard HPLC analysis . Histamine concentration in the wine sample stored in the glass bottle was not revealed by HPLC, probably since the HPLC limit of detection, in the used experimental conditions, does not allow revealing nanomolar concentration of histamine.…”

Simultaneous separation and determination of seven chelating agents using high‐performance liquid chromatography based on statistics design

“…Dethier et al. explored a method of HPLC–UV detection in which two diastereoisomers with similar molecular structures were separated and identified after pre‐column derivatization with dansyl chloride. Gheshlaghi et al.…”

“…In contrast, statistical experimental design can effectively optimize the analysis conditions [21,22]. Dethier et al [23] explored a method of HPLC-UV detection in which two diastereoisomers with similar molecular structures were separated and identified after pre-column derivatization with dansyl chloride. Gheshlaghi et al [24] used statistical design coupled with reverse-phase HPLC to optimize the separation of 20 amino acids, setting overall resolution as the response variable.…”

New Spectrofluorimetric Method for Determining Cadaverine Following Derivation with Orthophthalaldehyde: Application in Fish Tissue

“…This can explained why many analysis methods have been developed for the detection and quantification of biogenic animes in fish and fish products. These methods include, thin layer chromatography (Shalaby, 1995); high pressure liquid chromatography (Li et al, 2013., Önal, Tekkeli, & Önal, 2013., de Figueiredo et al, 2015., Ibarra et al,2015., Lee, Yoo, & Shin, 2015, gas chromatography (Khuhawar, Memon, & Bhanger, 2000), (Almeida, Fernandes, & Cunha, 2012) and capillary zone electrophoresis (Arce et al, 1997). The revision of these chromatographic methods by Önal (Önal, Tekkeli, & Önal, 2013) shows that they often require sophisticated equipment with trained staff.…”