HPTLC analysis of Withaferine A from an herbal extract and polyherbal formulations

Abstract

Withania somnifera has been used in Ayurvedic medicine for treatment of depression and inflammation, and as an aphrodisiac. It contains many phytochemicals such as Withaferine A, withanine, anahygrine, tropine, and withanolides. Of these, withaferine A is considered to be the most active compound. Withaferine A was estimated in herbal extract and polyherbal formulations by high performance thin layer chromatography (HPTLC). As there is no official HPTLC protocol for quantitation of the above phytochemicals, an attempt was made to quantify withaferine A in herbal extract and polyherbal formulations produced from Withania somnifera. Precoated silica gel G (aluminium backed) plates were used as stationary phase and toluene:ethyl acetate: formic acid (50 : 15 : 5) was used as mobile phase. Detection and quantification were performed by densitometry at k 213 nm. The linear range was 1 lg to 3 lg. This HPTLC method was found to be reproducible, accurate, and precise.

Withanolides against TLR4‐Activated Innate Inflammatory Signalling Pathways: A Comparative Computational and Experimental Study

“…Also, the quality of the crude drug used in manufacturing of herbal formulations depends upon the active ingredients present in them (Izzo et al ., ; Thornfeldt and Rizer, ). High‐performance thin layer chromatography (HPTLC) is a sensitive and accurate method for determination of withanolides quantity in Withania plants (Mahadevan et al ., ; Sharma et al ., ; Nayak et al ., ). The W. somnifera extracts were subjected to withanolide quantification and were also analysed for their antiinflammatory activity in relation to their withanolide quantity.…”

Optimisation of a microwave‐assisted method for extracting withaferin A from Withania somnifera Dunal. using central composite design

“…The analysis of WA was reported in 2003 using the HPTLC technique (Mahadevan et al ., 2003). In order to modify this method various mobile phases were investigated in our laboratory for a reproducible resolution of WA via HPTLC.…”

Validated thin‐layer chromatographic method for the identification and monitoring of the effect of the extraction method on the yield and phytochemical constituents of Egyptian Withania somnifera leaves

“…Evaluation of W. somnifera is almost exclusively based on determination of the content of withanolides since most of the pharmacological activities of the plant are attributed to them. However, the reported methods focus mainly on the two major components of the Indian variety; withaferin A and withanolide A . One of these methods, stated in the USP38‐NF33 modified monograph depends on TLC identification of withanolide A, withanone, and withaferin A bands after derivatization .…”

“…Evaluation of W. somnifera is almost exclusively based on determination of the content of withanolides since most of the pharmacological activities of the plant are attributed to them. However, the reported methods focus mainly on the two major components of the Indian variety; withaferin A and withanolide A [2][3][4][5][6][7][8][9]. One of these methods, stated in the USP38-Abbreviations: Twc, total withanolide content; USAE, ultrasound-assisted extraction Conflict of interest: No potential conflict of interest was reported by the authors.…”