HPTLC Based Chemometrics of Medicinal Mushrooms

Bhardwaj, Anuja; Pal, Mamta; Srivastava, Mousami; Tulsawani, Rajkumar; Sugadev, R.; Misra, Kshipra

doi:10.1080/10826076.2015.1050501

Cited by 24 publications

(9 citation statements)

References 24 publications

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“…HPTLC analysis performed on aqueous, ethyl acetate, and methanolic extracts of G. lucidum fruiting body and mycelia proved the presence of antioxidant compounds viz. ascorbic acid, gallic acid, rutin, and quercetin [9]. A study performed on different solvent (acetone, chloroform, methanol, and aqueous) extracts of G. lucidum mycelium showed encouraging results against pathogenic bacterial growth [10], which was mainly attributed to its phenolic contents.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

Mishra

Joshi

Rajput

et al. 2018

Advances in Pharmacological Sciences

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Ganoderma lucidum (G. lucidum) fungus (Family Ganodermataceae) is widely used as a traditional medicine in China, Japan, and many Asian countries on account of its numerous medicinal properties such as antioxidant, anticancer, antimicrobial, energy enhancing, and immunostimulatory. This broad spectrum of therapeutic effects exhibited by G. lucidum is ascribed to its abundance in several classes of chemical constituents, namely, carbohydrates, flavonoids, minerals, phenolics, proteins, and steroids which possess substantial bioactivities. The aim of the current study was to prepare phenolic rich fractions (PRFs) from aqueous extract of the Indian variety of G. lucidum mycelium and fruiting body. These fractions were assessed for their antioxidant capacity by TPC (total phenolic content), TFC (total flavonoid content), FRAP (ferric reducing antioxidant power), and ABTS [2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid] assays. Quantification of flavonoids and nucleobases present in the fractions was carried out by high-performance thin layer chromatography (HPTLC). The antibacterial activity of the fractions was evaluated against Escherichia coli, Salmonella typhi, and Staphylococcus aureus. The antibacterial mechanism of action of the PRFs was established to be generation of reactive oxygen species and leakage of proteins within bacterial cells. Additionally, the protective effect of the PRFs in counteracting hypoxia was observed in HEK 293 cell lines.

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Section: Introductionmentioning

confidence: 99%

Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

Mishra

Joshi

Rajput

et al. 2018

Advances in Pharmacological Sciences

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“…24 Hesperidin, a member of the flavonoid group, is found in mushrooms such as Cordyceps sinensis and Ganoderma lucidum in addition to citrus species. 25 It has been reported that hesperidin Catechins exhibited significant antioxidant, anticancerogenic, anti-inflammatory, thermogenic, antimutagenic, probiotic and antimicrobial properties in the result of in vivo and in vitro studies. 27 Syringic acid is known to exhibit multiple bioactive properties such as strong antioxidant, antiproliferative, antiendotoxic, antimicrobial, anti-inflammatory and anti-cancer effects.…”

Section: Phenolic Contentsmentioning

confidence: 99%

Phenolic Contents, Oxidant/Antioxidant Potential and Heavy Metal Levels in Cyclocybe cylindracea

Sevindik¹,

Akgül²,

Bal³

et al. 2018

IJPER

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Introduction: Mushroom species have been used for medical purposes and as nutrients since the old times. The present study aimed to determine phenolic content, antioxidant activity and heavy metal content of Cyclocybe cylindracea (DC.) Vizzini & Angelini mushroom. Materials and Methods: Phenolic contents were screened with HPLC devices. TAS, TOS and OSI values were determined using Rel Assay kits. Heavy metal content were determined with atomic absorption method using an atomic absorption spectrophotometer. Result and Discussion: Gallic acid, hesperidin, catechin, syringic acid and hydroxybenzoic acid were detected in the mushroom. It was determined that the heavy metal content in C. cylindracea were generally at optimal levels and the TOS value of mushroom was high. Conclusion: C. cylindracea could be consumed as a good antioxidant source as long as it is obtained from safe areas.

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“…Up to now, in order to evaluate the properties of the raw materials of edible and medicinal mushrooms, many qualitative and quantitative techniques were established with the aid of chemometrics 15 , 17 , 36 , 37 . For the present study, the potential use of multi-spectral information fusion with the aid of chemometric methods as a tool to rapidly and comprehensively assess different properties of wild and cultivated W. cocos raw materials from diverse geographical origins has been successfully investigated.…”

Section: Resultsmentioning

confidence: 99%

Differentiation and comparison of Wolfiporia cocos raw materials based on multi-spectral information fusion and chemometric methods

Wang

2018

Sci Rep

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In order to achieve the target of deeper insight into the differentiation and comparison of Wolfiporia cocos, a total of 350 samples including distinct growth patterns, various collection regions and different medicinal parts were investigated using multi-spectral information fusion based on ultraviolet (UV) and Fourier transform infrared (FT-IR) spectroscopies coupled with chemometrics. From the results, the discrimination of samples was obtained successfully and good classification performances were shown according to partial least squares discriminant analysis (PLS-DA) models. Comparatively, the distinctness of chemical information in the two medicinal parts of W. cocos were much more than that in the same part with different growth patterns and collection areas. Meanwhile, an interesting finding suggested that growth patterns rather than geographical origins could be the dominant factor to effect the chemical properties of the same part samples, especially for the epidermis. Compared with the epidermis samples, there were better quality consistency for the inner part of W. cocos. Totally, this study demonstrated that the developed method proved to be reliable to perform comparative analysis of W. cocos. Moreover, it could provide more comprehensive chemical evidence for the critical supplement of quality assessment on the raw materials of W. cocos.

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HPTLC Based Chemometrics of Medicinal Mushrooms

Cited by 24 publications

References 24 publications

Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

Phenolic Contents, Oxidant/Antioxidant Potential and Heavy Metal Levels in Cyclocybe cylindracea

Differentiation and comparison of Wolfiporia cocos raw materials based on multi-spectral information fusion and chemometric methods

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