HPTLC Determination of Betamethasone in Tablets and Its Validation

Abstract

A simple and rapid high performance thin layer chromatography (HPTLC) densitometric method has been developed for determination of betamethasone base in the tablets. After extraction of the analyte with 96% ethanol, the extracts were spotted on precoated HPTLC silica gel plates, which were then developed with a mixture of chloroformmethanol-water (18 : 5 : 0.5). Quantitative evaluation was performed by measuring the absorbance reflectance of the analyte spots at 245 nm. The densitometric method is selective, precise, and accurate and can be used for routine analysis of the tablet preparations in pharmaceutical industry quality control laboratories.

Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

“…A sequence of betamethasone dilutions was prepared (from 0 to 5 mg mL −1 in PBS) to obtain a calibration curve and assesses the amount of betamethasone released. The UV absorbance at 245 nm [ 27 ] was read in a microplate reader to measure the betamethasone release (EMax; Molecular Devices, Sunnyvale, CA, USA). Three samples per condition were evaluated at each time point.…”

Determination of Inulin From Multivitamin Syrup Product by High Performance Liquid Chromatography With Ri Detector

“…Linearity of inulin was achieved from 0.5 to 3.5 mg/mL with line equation Y = 695360.5x -8865.7. The relative process standard deviation (V xo ) and Xp values of inulin were 2% and 0.12 mg/mL [9][10], (n = 6; r = 0.999), respectively. ANOVA regression-test for testing linearity of the regression line showed a significant calculated F-value (11317.96 for p < 0.0001).…”

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…64 In the majority of cases, silica gel 60 F254 plates were used for the assay of steroid preparations. Some of the investigated formulations are as follows (the wavelength of the densitometric scan in parentheses): finasteride tablets (228 nm), 65 betamethasone tablets (245 nm), 66 mometasone furoate topical preparations (260 nm), 67 cyproterone acetate-ethinylestradiol tablets (284 nm) 68 or ethinylestradiol (220 nm) and cyproterone acetate (284 nm), 69 dexamethasone and xylometazoline in nasal drops (240 nm), 70 dexamethasone sodium phosphate-neomycin sulfate solution (246 nm), 71 prednisolone acetate in pharmaceutical formulations (243 nm; stepwise gradient). 72 The basis for the assay of hydrocortisone, hydrocortisone acetate and clioquinol containing ointment and cream preparations was fluorescence quenching at 254 nm.…”