HPTLC fingerprint identification of commercial ginseng drugs ‐ reinvestigation of HPTLC of ginsenosides

Xie, Peng; Yan, Yi

doi:10.1002/jhrc.1240101106

Cited by 18 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High-Performance Thin-Layer Chromatography (HPTLC) had its glorious past in the second half of last century [1][2][3][4][5][6][7][8][9][10]. As identification of herbal medicines, HPTLC is adopted widely in the monographs of the pharmacopeias in various countries.…”

Section: Introductionmentioning

confidence: 99%

Value the Unique Merit of HPTLC Image Analysis and Extending its Performance by Digitalization for Herbal Medicines Quality Control

Xie¹,

Sun²,

Xu³

et al. 2014

J Chromatogr Sep Tech

View full text Add to dashboard Cite

It is well known that High-Performance Thin Layer-Chromatography (HPTLC), the off-line planar chromatographic technique, has been employed in the pharmacopoeias of many countries for identification by virtue of its low-cost, less dependent on expensive equipment, flexible mobile phase composition and easy post-derivatization. In particular, the unique merit of HPTLC is able to provide the picture-like images of multiple samples in parallel on the same plate for instant viewing. In addition to the two dimension parameters (migration distance (R f value), integration peak value), the attractive color image, as the third dimension parameter, enhances the potency of HPTLC identification. The bioactivity of herbal medicines released from its compound bioactive ingredients in a holistic manner. Therefore the detected total chemical composition in the image should be reasonably more meaningful to assessing the inherent quality than only any selected single marker constituent of the given herbal drug. A vivid colorful picture-like HPTLC image could be easily recognized but not described properly by words. Hence extending the plate spot/band capacity on the plate for involving the bioactive components as much as possible. Combining digitizing the HPTLC image, the acquired infographic (HPTLC image, the digital scanning profile and the integrated parameters) can be used for establishing the fingerprint common pattern of the given species. It can be coupled with chemometrics analysis for more effectively quantifiable assessing the inherit quality of the herbal drugs. In this paper we reported methodically some Chinese herbal drugs analysis via various levels for demonstration of the practical application in QC.

show abstract

Section: Introductionmentioning

confidence: 99%

Value the Unique Merit of HPTLC Image Analysis and Extending its Performance by Digitalization for Herbal Medicines Quality Control

Xie¹,

Sun²,

Xu³

et al. 2014

J Chromatogr Sep Tech

View full text Add to dashboard Cite

show abstract

“…Layer conditioning before plate development is an important factor affecting the efficiency of chromatographic systems and separation selectivity in thin-layer chromatography [2]. Separation results are affected by the kind of solvent used for chamber saturation and the layer conditioning time.…”

Section: Introductionmentioning

confidence: 99%

“…Silica layers are sometimes deactivated with ammonia vapour before chromatography [2,28]. Conditioning of the plate with ammonia before development markedly improved separation of protoberberine alkaloids when mobile phase containing ammonia was used for their separation [2].…”

Section: Introductionmentioning

confidence: 99%

Effect of the vapour phase on the separation of isoquinoline alkaloids by thin-layer chromatography

Petruczynik¹,

Sliwka²,

Waksmundzka‐Hajnos³

2010

Acta Chromatographica

View full text Add to dashboard Cite

The effect of conditioning of the silica layer by mobile phase vapour, diethylamine vapour and its aqueous and methanolic solutions, and ammonia vapour on the retention of alkaloids eluted with multicomponent non-aqueous mobile phases was examined. Effect of conditioning time and vapour phase composition on system efficiency and peak symmetry was investigated, as also was the effect of vapour phase composition on separation selectivity. The most effective and selective systems were used for separation of the alkaloid fraction from Fumaria officinalis.

show abstract

Phytopreventative effects of Gynostemma pentaphyllum against acute Indomethacin‐induced gastrointestinal and renal toxicity in rats

Hesse

Razmovski-Naumovski

Duke

et al. 2007

Phytotherapy Research

View full text Add to dashboard Cite

In the present study, the phytoprotective effects of gypenosides from Gynostemma pentaphyllum throughout the gastrointestinal tract and kidney were examined in indomethacin-treated rats. Indomethacin induced gastric and intestinal damage as well as renal toxicity after a single toxicological dose (10 mg/kg) in rats. Acute oral administration of the gypenoside extract (200 mg/kg) significantly reduced gastric and intestinal toxicity induced by indomethacin as measured by ulceration, caecal haemoglobin and plasma haptoglobin. A significant decrease in small intestinal lactose fermenting enterobacteria was evident in animals treated with indomethacin and those pre-treated with G. pentaphyllum then indomethacin. In the renal system, kidney toxicity was evident after indomethacin and in animals pre-treated with indomethacin plus G. pentaphyllum with an increase in urinary N-acetyl-beta-glucosaminidase and a decrease in urinary sodium and chloride electrolyte output. However, a significant increase in urinary microprotein in indomethacin-treated animals was not present in indomethacin plus G. pentaphyllum-treated animals. These studies demonstrate the efficacy of Gynostemma pentaphyllum in lowering gastrointestinal damage induced by indomethacin. The results suggest further investigations of Gynostemma gypenosides are warranted to examine the mechanisms of this phytoprotective activity.

show abstract

HPTLC fingerprint identification of commercial ginseng drugs ‐ reinvestigation of HPTLC of ginsenosides

Cited by 18 publications

References 9 publications

Value the Unique Merit of HPTLC Image Analysis and Extending its Performance by Digitalization for Herbal Medicines Quality Control

Value the Unique Merit of HPTLC Image Analysis and Extending its Performance by Digitalization for Herbal Medicines Quality Control

Effect of the vapour phase on the separation of isoquinoline alkaloids by thin-layer chromatography

Phytopreventative effects of Gynostemma pentaphyllum against acute Indomethacin‐induced gastrointestinal and renal toxicity in rats

Contact Info

Product

Resources

About