HRP-mediated polymerization forms tough nanocomposite hydrogels with high biocatalytic performance

Abstract

This communication describes the mild and quick construction of tough nanocomposite hydrogels via a horseradish peroxidase-mediated radical polymerization for effectively immobilizing enzymes to attain high catalytic performance in various solvents.

In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity

“…In general, AgNP biomaterials have been fabricated mainly through three methods: admixture 28 , surface coating or adsorption 29,30 , and complexing or covalent cross-linking with modified AgNPs 31,32 . However, there are several distinct disadvantages with these methods.…”

Printable hybrid hydrogel by dual enzymatic polymerization with superactivity

“…It should be noted that during the polymerization/cross-linking process, the dual enzymes (GOx/HRP) not only generate radicals in the initiation stage,33 but also immobilize them into the hybrid hydrogel matrix in situ . To explore the catalytic activity of free enzymes versus immobilized enzymes, the oxidation of o -phenylenediamine (OPD) by H 2 O 2 was selected as a model reaction with glucose and OPD as substrates 33,34. Regardless of whether the enzymes were immobilized or not, all tests concerning catalysis were performed with identical concentrations of GOx and HRP (GOx, 5 mg L –1 ; HRP, 2.1 mg L –1 ).…”

Supercritical fluid immobilization of horseradish peroxidase on high surface area mesoporous activated carbon

“…In addition, the advantageous transport properties of scCO 2 (low viscosity and high diffusivity) favors the transport of HRP through the porous matrix of the carbon [31]. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 obtained a maximum loading of 7% (13.8 mg g -1 ) on periodic mesoporous organosilica (S BET ~ 710 m 2 /g) at 4°C in the aqueous medium [42].…”

“…Additionally, the extent of reduction in M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 mesopore area for the synthesized (mesoporous) carbons is indicative of the higher uptake of HRP compared to that for commercial microporous carbon. The large dimensions of HRP (37 × 43 × 64 (Å)) [42] increases the likelihood that it is adsorbed on to mesopores (20-500 Å) rather than on micropores (<20 Å) [17,28,43].…”