Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells

Abstract

MicroRNAs (miRNAs) are 22- to 25-nucleotide non-coding RNA molecules that function as negative regulators of gene expression. In previous years, increasing evidence has arisen implicating the involvement of miRNAs in carcinogenesis. In previous studies, the role of miRNA-101 (miR-101) in tumors has been identified as a tumor suppressor and, until now, the role of miR-101 and Rac1 in thyroid cancer has remained undefined. This study revealed that miR-101 is significantly downregulated in papillary thyroid carcinoma (PTC) tissue and thyroid cancer cell lines, and that the downregulated miR-101 is associated with lymph node metastasis. Infection with the miR-101 murine stem cell virus may markedly inhibit cell migration and invasion in TPC-1 and HTH83 thyroid cancer cell lines. Rac1 was demonstrated to be negatively regulated by miR-101 at the post-transcriptional level, via a specific target site within the 3′ untranslated region by dual-luciferase reporter assay. The expression of Rac1 was also observed to inversely correlate with miR-101 expression in PTC tissues; knockdown of Rac1 by shRNA inhibited thyroid cancer cell migration and invasion, resembling that of miR-101 overexpression. Thus, these findings suggested that miR-101 acts as a novel suppressor by targeting the Rac1 gene and inhibiting thyroid cancer cell migration and invasion.

Upregulation of miR-494 Inhibits Cell Growth and Invasion and Induces Cell Apoptosis by Targeting Cleft Lip and Palate Transmembrane 1-Like in Esophageal Squamous Cell Carcinoma

“…miRNAs can regulate gene expression by targeting complementary sequences primarily located on the 3 0 untranslated regions (UTRs) of target genes [11][12][13][14]. For example, microRNA (miR)-101 targets Rac1 to suppress the migration and invasion of thyroid cancer cells [15], and miR-708 and miR-31 regulate proliferation, apoptosis, and invasion of colorectal cancer cells [16]. Let-7 is downregulated in human lung cancer, and this downregulation is associated with shortened survival in these patients; however, restoration of Let-7 expression induces growth inhibition of lung cancer cells [17].…”

MiR-101 Targets the EZH2/Wnt/β-Catenin the Pathway to Promote the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

“…Further investigation is required to clarify whether the osteogenic differentiation is regulated by other miRNAs. MiR-101, frequently silenced in human cancers, has been shown to have fundamental roles in diverse cellular processes, especially in the control of proliferation and differentiation12131415. Notably, Li et al 16 reported that miR-101 may promote the osteogenic differentiation of periodontal ligament cells by targeting periodontal ligament associated protein-1 (PLAP-1/aspirin).…”

The Stature of Human Thyroid Cancer Related to Over Expressed mir-221 and mir-146b

“…These include immuno-histochemical stains, microRNAs, gene mutations/ rearrangements, and gene expression panels [5]. MicroRNAs (miRNAs) are 22-to 25-nucleotide non-coding RNA molecules that typically prohibit protein production by binding to the 3' untranslated region [UTR] of specific target mRNAs [6]. Each microRNA has the distinct capability to potentially regulate the expression of hundreds of coding genes and thereby modulate several cellular pathways including proliferation, apoptosis and stress response [7].…”