Hsa-miR-1246 and hsa-miR-1290 are associated with stemness and invasiveness of non-small cell lung cancer

Kim, Gwangil; An, Hee-Jung; Lee, Mi Jung; Song, Ji‐Hyun; Jeong, Jooyeon; Lee, Ji‐Hyun; Jeong, Hye-Cheol

doi:10.1016/j.lungcan.2015.11.013

Cited by 98 publications

(82 citation statements)

References 29 publications

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“…Twelve upregulated miRNAs were identified as the most significant ones using p < 0.05 and 2-fold expression difference level as cutoff values. miR-1290 has been indicated as a novel biomarker for early detection, recurrence, and prognosis of colon cancer and mediates chemoresistance to paclitaxel in hepatocellular carcinoma 21 . Both miR-1290 and miR-3138 could promote radio resistance of human cervical cancer cells 22 .…”

Section: Discussionmentioning

confidence: 99%

“…In non-small-cell lung cancer (NSCLC), high expression of miR-1290 was detected in tissues and serum and was closely correlated with lymph node metastasis, tumor/node/metastasis (TNM) stage, and poor prognosis 21, 28. miR-1290 overexpression promotes proliferation, migration, and invasion in esophageal squamous cell carcinoma (ESCC) by targeting NFIX 29 .…”

Section: Discussionmentioning

confidence: 99%

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miR-1290 Is a Biomarker in DNA-Mismatch-Repair-Deficient Colon Cancer and Promotes Resistance to 5-Fluorouracil by Directly Targeting hMSH2

Jiang

Shao

et al. 2017

Molecular Therapy - Nucleic Acids

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5-Fluorouracil (5FU)-based adjuvant therapy is the first-line therapy for treating stage II and III colon cancer after surgery. However, its therapeutic efficacy is limited because of chemoresistance, especially in deficient mismatch repair (dMMR) colon cancer. Here, we first used laser capture microdissection to obtain purified cells from four dMMR and four proficient mismatch repair (pMMR) colon cancer tissues. Second, microRNA (miRNA) microarray chips were used to identify miRNAs that are differentially expressed between these two classes of tumors. Third, we analyzed their differential expression by qRT-PCR in a panel of 5-FU-resistant colon cancer cell lines. We identified that miR-1290 was one of the most upregulated miRNAs in both dMMR colon cancer tissues and 5-FU-resistant cells. We also found that miR-1290 was positively correlated with dMMR status and predicted poor prognosis in stage II and III colon cancer patients who received 5-FU-based chemotherapy. Furthermore, we demonstrated that inhibition of the expression of miR-1290 enhanced sensitivity to 5-FU treatment in vitro and in tumor xenografts in vivo by direct targeting hMSH2. Our study indicates that miR-1290 may become a promising biomarker of dMMR colon cancer and predicts the prognosis of stage II and III patients who receive 5-FU-based adjuvant therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

miR-1290 Is a Biomarker in DNA-Mismatch-Repair-Deficient Colon Cancer and Promotes Resistance to 5-Fluorouracil by Directly Targeting hMSH2

Jiang

Shao

et al. 2017

Molecular Therapy - Nucleic Acids

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“…The miRNA miR-1246 has been reported as a possible biomarker in esophageal squamous cell carcinoma58, colorectal cancer59 and cervical cancer60, and its antagonism led to suppression of proliferation, colony formation and invasion in non-small cell lung cancer cell lines61. Conversely, very little is known regarding the functions of miR-1268, other than that it is expressed in gestational diabetes mellitus62, and may be implicated in facioscapulmohumeral muscular dystrophy63.…”

Section: Discussionmentioning

confidence: 99%

Exosomes derived from embryonal and alveolar rhabdomyosarcoma carry differential miRNA cargo and promote invasion of recipient fibroblasts

Ghayad

Rammal

Ghamloush

et al. 2016

Sci Rep

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Rhabdomyosarcoma (RMS) is an aggressive childhood soft tissue tumor, which exists in oncoprotein PAX-FOXO1 fusion positive and fusion negative subtypes, with the fusion-positive RMS being characterized by a more aggressive clinical behavior. Exosomes are small membranous vesicles secreted into body fluids by multiple cell types, including tumor cells, and have been implicated in metastatic progression through paracrine signaling. We characterized exosomes secreted by a panel of 5 RMS cell lines. Expression array analysis showed that, for both fusion-positive and fusion-negative cells, exosome miRNA clustered well together and to a higher extent than cellular miRNA. While enriched miRNA in exosomes of fusion-negative RMS cells were distinct from those of fusion-positive RMS cells, the most significant predicted disease and functions in both groups were related to processes relevant to cancer and tissue remodelling. Functionally, we found that RMS-derived exosomes exerted a positive effect on cellular proliferation of recipient RMS cells and fibroblasts, induced cellular migration and invasion of fibroblasts, and promoted angiogenesis. These findings show that RMS-derived exosomes enhance invasive properties of recipient cells, and that exosome content of fusion-positive RMS is different than that of fusion-negative RMS, possibly contributing to the different metastatic propensity of the two subtypes.

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“…Accumulating evidence has indicated that miRNAs are important regulatory factors involved in various biological processes, including cell differentiation, growth, metastasis and apoptosis (8,9). Aberrant expression of miRNAs has been identified in numerous cancers, including lung and breast cancer and hepatocellular carcinoma (10)(11)(12)(13)(14)(15). Numerous miRNAs may be regarded as potential and novel biomarkers for the diagnosis, therapy and prognosis of numerous types of cancer (16,17).…”

Section: Introductionmentioning

confidence: 99%

miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer

Guo

Xiao

et al. 2017

Oncol Lett

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Abstract. MicroRNAs are small non-coding RNAs, which are critical regulators of carcinogenesis and tumor progression. Previous studies have identified that microRNA-20b (miR-20b) acts as an oncogene in numerous cancers. However, the role of miR-20b in prostate cancer remains unclear. The present study aimed to investigate the expression of miR-20b in prostate cancer and to examine whether modulating miR-20b expression impacts prostate cancer cellular proliferation and migration. It was revealed that miR-20b was strongly expressed in prostate cancer tissues compared with adjacent normal prostate tissues (P<0.05). Knockdown of miR-20b expression by miR-20b inhibitor inhibited VCaP and PC-3 cell growth and migration. Through bioinformatics analysis, phosphatase and tensin homolog (PTEN) was predicted as a target gene of miR-20b in prostate cancer cells, which was validated by dual-luciferase reporter assay and western blot analysis. In addition, restoration of PTEN expression levels did not affect endogenous miR-20b expression in prostate cancer cells. In conclusion, the present study indicated that miR-20b promotes cellular proliferation and migration by directly regulating PTEN in prostate cancer.

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Hsa-miR-1246 and hsa-miR-1290 are associated with stemness and invasiveness of non-small cell lung cancer

Cited by 98 publications

References 29 publications

miR-1290 Is a Biomarker in DNA-Mismatch-Repair-Deficient Colon Cancer and Promotes Resistance to 5-Fluorouracil by Directly Targeting hMSH2

miR-1290 Is a Biomarker in DNA-Mismatch-Repair-Deficient Colon Cancer and Promotes Resistance to 5-Fluorouracil by Directly Targeting hMSH2

Exosomes derived from embryonal and alveolar rhabdomyosarcoma carry differential miRNA cargo and promote invasion of recipient fibroblasts

miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer

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