Hsa_Circ_0001206 is downregulated and inhibits cell proliferation, migration and invasion in prostate cancer

Abstract

Our study is to explore the expression profiles and potential functions of circRNAs in prostate cancer (PCa). A total of 95 circRNAs and 830 mRNAs were screened to be significantly differentially expressed in PCa tissues by microarrays. Co-expression and competitive endogenous RNA (ceRNA) network were constructed to reveal the potential regulatory mechanisms of circRNAs. Three circRNAs, hsa_circ_0001206, hsa_circ_0001633, and hsa_circ_0009061 were validated to be down-regulated in PCa by quantitative real-time PCR (qRT-PCR) and hsa_circ_0001206 as well as hsa_circ_0009061 was significantly associated with clinical features of PCa patients. Meanwhile, Receiver Operating Characteristic (ROC) curves showed their good diagnostic value as biomarkers for PCa. The down-regulation of hsa_circ_001206 was partly because of the regulation of DExH-Box Helicase 9 (DHX9). Moreover, overexpression of hsa_circ_0001206 inhibited PCa cell proliferation, migration, and invasion in vitro and prevented tumor growth in vivo. Dual-luciferase reporter assays showed hsa_circ_0001206 could directly bind to miR-1285-5p. The expression of Smad4, a well-known suppressive gene in PCa, can be increased by overexpression of hsa_circ_0001206 and this effect could be partly reversed by co-transfection of miR-1285-5p mimic. The study revealed expression profiles and potential functions of circRNAs and demonstrated hsa_circ_0001206 played a suppressive role in the pathogenesis of PCa. ARTICLE HISTORY

Circ‐MTO1 correlates with favorable prognosis and inhibits cell proliferation, invasion as well as miR‐17‐5p expression in prostate cancer

“…For instance, circ‐ABCC4 advocates the progression of prostate cancer by acting as competing endogenous RNA (ceRNA) of miR‐1182 via promoting the expression of forkhead box P4 . Another study reveals that circ_0001206 is decreased in prostate cancer tumor tissue and represses cell proliferation, migration and invasion in prostate cancer cells . Additionally, a recent research discloses that the decreased circ‐itchy E3 ubiquitin‐protein ligase associates with more severe pathological T stage, increased lymph node metastasis risk and worse survival in patients with prostate cancer .…”

“…12 Another study reveals that circ_0001206 is decreased in prostate cancer tumor tissue and represses cell proliferation, migration and invasion in prostate cancer cells. 17 Additionally, a recent research discloses that the decreased circ-itchy E3 ubiquitin-protein ligase associates with more severe pathological T stage, increased lymph node metastasis risk and worse survival in patients with prostate cancer. 18 In terms of the specific circRNA (circ-MTO1) evaluated in our study, the circ-MTO1, it has not been studied in prostate cancer before.…”

Downregulated hsa_circ_0077837 and hsa_circ_0004826, facilitate bladder cancer progression and predict poor prognosis for bladder cancer patients

“…Recent evidences have suggested that circRNAs involve in far‐ranging biological processes, including transcription, mRNA splicing, RNA decay and translation, and their deregulation takes pivotal roles in the development and metastasis of various tumors, such as glioma, 15 hepatoblastoma, 16 colorectal cancer, 17 lung cancer, 18 prostate cancer, 19 bladder cancer, 20 and gastric cancer 21 . For example, Li et al revealed that circANKS1B promotes colorectal cancer cell migration and invasion by working as a molecular sponge of miR‐149 to regulate FOXM1 and Slug protein expressions 17 .…”

Circular RNAs and Their Linear Transcripts as Diagnostic and Prognostic Tissue Biomarkers in Prostate Cancer after Prostatectomy in Combination with Clinicopathological Factors

“…Several publications particularly focused on androgen receptor pathway related circRNAs [54][55][56][57]. Correlations between the expression of circRNAs and relevant clinicopathological factors or survival Kaplan-Meier analyses have been reported [44,51,[58][59][60][61][62]. However, it is astonishing that the potential of circRNAs as diagnostic and prognostic tissue biomarkers has so far only been evaluated in isolated cases [47,58,61].…”

“…Correlations between the expression of circRNAs and relevant clinicopathological factors or survival Kaplan-Meier analyses have been reported [44,51,[58][59][60][61][62]. However, it is astonishing that the potential of circRNAs as diagnostic and prognostic tissue biomarkers has so far only been evaluated in isolated cases [47,58,61]. As far as we know, only one study has ever conducted multivariate analyses of circRNAs in connection with clinicopathological factors [61].…”