Hsa_circ_0060927 participates in the regulation of Caudatin on colorectal cancer malignant progression by sponging miR‐421/miR‐195‐5p

Chen, Juan; Xu, Li Chong; Fang, Mingzhi; Xue, Ya-Hong; Cheng, Yu-Shan; Tang, Xiuhong

doi:10.1002/jcla.24393

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…In fact, two independent studies in glioma, another type of brain tumor [82,83], showed an overexpression of circHIPK3 and its involvement in the disease by reducing the expression of miR-421 and miR-124, which prevent cell proliferation and growth through CDK6 inhibition [84]. Additionally, these miRNAs seem to be downregulated in different cancers, such as colorectal cancer, confirming the hypothesis that circHIPK3 upregulation may reduce these miRNA levels [85]. Those miRNAs are commonly downregulated in MB, which does not fit with the proposed hypothesis that this is mediated by the upregulation of circHIPK3, as it has been shown in different cancers.…”

Section: Discussionmentioning

confidence: 77%

The Role of the Dysregulation of Long Non-Coding and Circular RNA Expression in Medulloblastoma: A Systematic Review

Martinez de Estibariz,

Jakjimovska,

Illarregi

et al. 2023

Cancers

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Medulloblastoma (MB) is the most common malignant brain tumor in childhood. Although recent multi-omic studies have led to advances in MB classification, there is still room for improvement with regard to treatment response and survival. Therefore, identification of new and less invasive biomarkers is needed to refine the diagnostic process and to develop more personalized treatment strategies. In this context, non-coding RNAs (ncRNAs) could be useful biomarkers for MB. In this article, we reviewed the role of two types of ncRNAs, long non-coding (lncRNAs) and circular RNAs (circRNAs), as biomarkers for the diagnosis, subgroup classification, and prognosis of MB. We also reviewed potential candidates with specific functions and mechanisms of action in the disease. We performed a search in PubMed and Scopus using the terms (“long non coding RNAs” OR ”lncRNAs”) and (“circular RNAs” OR ”circRNAs”) AND ”medulloblastoma” to identify biomarker discovery or functional studies evaluating the effects of these ncRNAs in MB. A total of 26 articles met the inclusion criteria. Among the lncRNAs, the tumorigenic effects of the upregulated lnc-IRX3-80 and lnc-LRRC47-78 were the most studied in MB. Among the circRNAs, the upregulation of circSKA3 and its functional impact in MB cell lines were the most consistent results, so this circRNA could be considered a potential biomarker in MB. Additional validation is required for many deregulated lncRNAs and circRNAs; therefore, further studies are warranted.

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Section: Discussionmentioning

confidence: 77%

The Role of the Dysregulation of Long Non-Coding and Circular RNA Expression in Medulloblastoma: A Systematic Review

Martinez de Estibariz,

Jakjimovska,

Illarregi

et al. 2023

Cancers

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“…It has been reported that has-mir-421 is closely related to lung adenocarcinoma. The results of Chen et al [38] showed that the increased expression level of has-mir-421 in plasma can significantly distinguish normal people, especially in early lung adenocarcinoma. However, has-mir-181b-3P, has-mir-337-3P, and has-mir-548d-3P have not been reported in lung adenocarcinoma, which is worthy of further study on their interaction with hsa_circ_0061817.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of hsa_circ_0061817 Can Inhibit the Proliferation and Invasion of Lung Cancer Cells Based on Active Compounds

Zhong

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. This study was aimed at investigating the expression level of hsa_circ_0061817 in lung adenocarcinoma cells and its effect on cell proliferation and invasion and the possible mechanism of hsa_circ_0061817 in lung adenocarcinoma. Methods. The overexpression plasmids of hsa_circ_0061817 (OE-hsacirc_0061817) were transfected into human lung A549 cells and mouse LLC-LUC cells, respectively. The cell viability was detected by CCK-8, and the cell proliferation was detected by cell clone formation assay and EdU assay. Transwell test was used to detect the ability of cell invasion, and apoptosis was detected by flow cytometry. WB was applied to determine the expression of apoptosis and epithelial mesenchymal transition-(EMT-) related proteins and also target proteins for observation the effect of OE-hsa_circ_0061817 on the growth of A549 cells in nude mice. Bioinformatics method was used to predict the binding microRNA (miRNA) of hsa_circ_0061817 and construct the regulatory network of competitive endogenous RNA (ceRNA) and functional analysis of miRNA target genes. Results. Compared with PLO-ciR group, the cell viability, proliferation, and invasive ability of A549 and LLC-LUC were significantly reduced in OE-hsa_circ_00061817 group, while the apoptosis increased in OE-hsa_circ_00061817 group compared to PLO-ciR group. WB results showed that the expression of caspase 3, caspase 7, caspase 9, and E-cadherin increased significantly, while the expression levels of vimentin and N-cadherin decreased severely. Most importantly, OE-hsa_circ_00061817 inhibited the growth of A549 tumor-bearing nude mice. According to TargetScan and mirBase databases, hsa_circ_0061817 may competitively bind hsa_mir-181b-3p, hsa-mir-337-3p, hsa-mir-421, and hsa-mir-548d-3p. The results of functional enrichment showed that miRNA target genes were involved in many cancer-related biological processes, including negative regulation of apoptosis, gene expression, transcriptional imbalance in cancer, transforming growth factor-β, and P53 signal pathway. Conclusions. Over expression of hsa_circ_0061817 inhibits the proliferation of lung adenocarcinoma A549 and LLC-LUC cells and may reduce the invasive ability of lung adenocarcinoma cells by weakening the process of EMT, which provides a new target for the prevention and treatment of lung adenocarcinoma.

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Hsa_circ_0060927 participates in the regulation of Caudatin on colorectal cancer malignant progression by sponging miR‐421/miR‐195‐5p

Chen

Fang

et al. 2022

Clinical Laboratory Analysis

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Background Caudatin is extracted from radix cynanchi bungei and has an inhibitory effect on cancer progression. The study aims to reveal the impacts of hsa_circ_0060927 on Caudatin‐mediated colorectal cancer (CRC) development and the underneath mechanism. Methods The expression levels of hsa_circ_0060927, microRNA‐421 (miR‐421) and miR‐195‐5p were detected by quantitative real‐time reverse transcription‐polymerase chain reaction. The protein expression was analyzed by Western blot or immunohistochemistry assay. Cell viability and proliferation were analyzed by 3‐(4,5)‐dimethylthiahiazo (‐z‐y1)‐3,5‐di‐phenytetrazoliumromide or 5‐Ethynyl‐29‐deoxyuridine assay. Cell apoptosis was quantified by flow cytometry analysis. Cell migration and invasion were investigated by transwell assay. The putative associations among hsa_circ_0060927, miR‐421 and miR‐195‐5p were predicted by the starbase online database, and identified by dual‐luciferase reporter, RNA pull‐down and RNA immunoprecipitation (RIP) assays. The impacts of Caudatin treatment on tumor growth in vivo were revealed by a xenograft tumor model assay. Results Hsa_circ_0060927 expression was significantly upregulated, whereas miR‐421 and miR‐195‐5p were downregulated in CRC tissues and cells compared with control groups. Hsa_circ_0060927 expression was closely associated with lymph node metastasis and tumor‐node‐metastasis stage. Caudatin treatment significantly decreased hsa_circ_0060927 expression but increased miR‐421 and miR‐195‐5p expression. Caudatin exposure suppressed CRC cell proliferation, migration and invasion, and induced cell apoptosis; however, hsa_circ_0060927 overexpression hindered these impacts. Additionally, hsa_circ_0060927 was associated with miR‐421/miR‐195‐5p. Depletion of miR‐421 or miR‐195‐5p attenuated the influences of hsa_circ_0060927 silencing on CRC development. Furthermore, Caudatin treatment repressed tumor growth in vivo. Conclusion Caudatin inhibited CRC cell malignancy through the hsa_circ_0060927/miR‐421/miR‐195‐5p pathway, which provided a potential therapeutic agent for CRC.

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Hsa_circ_0060927 participates in the regulation of Caudatin on colorectal cancer malignant progression by sponging miR‐421/miR‐195‐5p

Cited by 7 publications

References 33 publications

The Role of the Dysregulation of Long Non-Coding and Circular RNA Expression in Medulloblastoma: A Systematic Review

The Role of the Dysregulation of Long Non-Coding and Circular RNA Expression in Medulloblastoma: A Systematic Review

Overexpression of hsa_circ_0061817 Can Inhibit the Proliferation and Invasion of Lung Cancer Cells Based on Active Compounds

Hsa_circ_0060927 participates in the regulation of Caudatin on colorectal cancer malignant progression by sponging miR‐421/miR‐195‐5p

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