HSP70-2 heat-shock protein of mouse spermatogenic cells

Eddy, Edward M.

doi:10.1002/(sici)1097-010x(199809/10)282:1/2<261::aid-jez28>3.0.co;2-v

Cited by 40 publications

(22 citation statements)

References 87 publications

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“…Heat-shock protein HSPA2 is expressed highly only in spermatogenic cells (Allen et al, 1988;Eddy, 1998), and mutational analysis demonstrated that it is required for CDC2A-cyclin B1 complex formation (Dix et al, 1996a;Dix et al, 1997;Zhu et al, 1997 Gt heterozygous spermatocytes (Fig. 7C,E).…”

Section: Research Article Meiotic Arrest In Eif4g3 Mutantsmentioning

confidence: 99%

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes

2010

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The ENU-induced repro8 mutation was identified in a screen to uncover genes that control mouse gametogenesis. repro8 causes male-limited infertility, with failure of spermatocytes to exit meiotic prophase via the G2/MI transition. The repro8 mutation is in the Eif4g3 gene, encoding eukaryotic translation initiation factor 4, gamma 3. Mutant germ cells appear to execute events of meiotic prophase normally, and many proteins characteristic of the prophase-to-metaphase transition are not obviously depleted. However, activity of CDC2A (CDK1) kinase is dramatically reduced in mutant spermatocytes. Strikingly, HSPA2, a chaperone protein for CDC2A kinase, is absent in mutant spermatocytes in spite of the presence of Hspa2 transcript, consistent with the observation that the repro8 phenotype is markedly similar to the phenotype of the Hspa2 knockout. Thus, EIF4G3 is required for HSPA2 translation in spermatocytes, a finding that provides the first genetic evidence for selective translational control of meiotic exit in mammalian spermatocytes.

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Section: Research Article Meiotic Arrest In Eif4g3 Mutantsmentioning

confidence: 99%

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes

2010

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“…Studies investigating the relationship between Hsp 70 expression and infertility are not frequently observed in literature. Present studies mainly deal with Hsp 70-2 , which is only constitutively expressed in experimental animals (18,19,41,42) and its human analogue Hsp A2, mostly in testes at different stages of spermatogenesis (22)(23)(24). However, the association of Hsp 70 (comprising both ihsp70 and hsc70) with semen quality is not clearly expressed in experimental animals and humans, and results obtained are conflicting.…”

Section: Figurementioning

confidence: 99%

“…The Hsp 70 family, with a molecular mass of 70 kDa, is the best studied class of Hsps (15)(16)(17). Hsp 70-2, which is a constitutively expressed member of the family, is shown to be especially important in murine spermatogenesis (18)(19)(20)(21). In addition, decreased expression of the Hsp A2 gene, known as the human homologue of the murine Hsp 70-2, has been reported to be associated with the pathogenesis of male infertility (22)(23)(24).…”

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confidence: 99%

The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation?

Erata

Toker

Durlanık

et al. 2008

Fertility and Sterility

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“…Altogether, these data suggest that the action of Brdt on hyperacetylated chromatin is crucial for the subsequent stages of genome reorganizations which could cooperate with tH2B/H2AL2 nucleosomes to mediate histone replacement by TPs. Additionally, we could show that in elongating spermatids, these TPs are chaperoned by HSPA2 [Govin et al 2006a], a factor which has been shown to also play a critical meiotic role by mediating the desynapsis of synaptonemal complexes [Eddy 1998]. It is also tempting to propose that HSPA2 also contributes to the exchange of histones by TPs and uses its ATPase domain to facilitate nucleosome disassembly and the incorporation of TPs.…”

Section: Identification and Functional Investigations Of Selected Facmentioning

confidence: 95%

Combined proteomic andin silicoapproaches to decipher post-meiotic male genome reprogramming in mice

Rousseaux¹,

Khochbin²

2012

Systems Biology in Reproductive Medicine

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During the post-meiotic maturation of the male gamete, the haploid genome undergoes major structural transitions, leading to its extreme compaction in a unique specialized structure. Although this process is highly conserved and crucial for the production of male gametes suitable for procreation, its molecular basis has remained unexplored for many years. In particular, the factors driving these events remain to be discovered. Our group has combined specific proteomic approaches with in silico analyses of available expression and sequence data in order to identify chromatin-associated factors as candidates involved in post meiotic reprogramming of the male genome of mice. Here we survey these approaches and the major results obtained. The systematic identification and the functional characterization of several of these factors has led to the proposal of the first working models, guiding us toward the understanding of this unique and very fundamental process, which has long remained one of the black boxes of modern biology.

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HSP70-2 heat-shock protein of mouse spermatogenic cells

Cited by 40 publications

References 87 publications

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes

The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation?

Combined proteomic andin silicoapproaches to decipher post-meiotic male genome reprogramming in mice

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