HSP70 and HSP90 are involved in shrimp Penaeus vannamei tolerance to AHPND-causing strain of Vibrio parahaemolyticus after non-lethal heat shock

Junprung, Wisarut; Supungul, Premruethai; Tassanakajon, Anchalee

doi:10.1016/j.fsi.2016.11.049

Cited by 81 publications

(37 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Effect of NLHS on shrimp survival upon VP AHpnD challenge. Herein, we have confirmed that treating shrimp with NLHS prior to VP AHPND infection results in a significantly higher survival rate, as previously demonstrated by Jungprung et al 12 . This survival experiment was set-up by dividing shrimps into four groups of non-heat treatment control (NH), NLHS control (NLHS), VP AHPND challenge (NH-VP), and NLHS plus VP AHPND challenge (NLHS-VP) ( Fig.…”

Section: Resultssupporting

confidence: 89%

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

Boonchuen

Maralit

Jaree

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

While Vibrio parahaemolyticus (Vp AHpnD) has been identified as the cause of early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) in shrimp, mechanisms of host response remain unknown. Understanding these processes is important to improve farming practices because this understanding will help to develop methods to enhance shrimp immunity. Pre-treatment of shrimp with 5-minute chronic non-lethal heat stress (NLHS) for 7 days was found to significantly increase Litopenaeus vannamei survival against Vp AHpnD infection. To elucidate the mechanism involved, mRNA and miRNA expression profiles from the hemocyte of L. vannamei challenged with Vp AHpnD after NLHS with corresponding control conditions were determined by RNA-Seq. A total of 2,664 mRNAs and 41 miRNAs were differentially expressed after the NLHS treatment and VP AHpnD challenge. A miRnA-mRNA regulatory network of differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs) was subsequently constructed and the interactions of DEMs in regulating the NLHSinduced immune-related pathways were identified. Transcriptomic data revealed that miRNA and mRNA interactions contribute to the modulation of NLHS-induced immune responses, such as the prophenoloxidase-activating system, hemocyte homeostasis, and antimicrobial peptide production, and these responses enhance Vp AHpnD resistance in L. vannamei. During its first outbreak, the devastating effect of early mortality syndrome (EMS) on the global shrimp industry was primarily caused by the lack of information regarding the disease and its causative agent 1. Later studies that were focused on mitigating this disease eventually identified the cause to be a toxin-harboring Vibrio parahaemolyticus, which causes Acute hepatopancreatic necrosis disease (AHPND) (VP AHPND) 2. This highlights how understanding disease etiology and a subsequent elucidation of host response can help to mitigate the effects of an outbreak. Understanding the functions of the shrimp immune system during disease progression is thus expected to create opportunities for the development of effective and efficient management strategies for VP AHPND infection. Likewise, information regarding the molecular mechanisms of AHPND tolerance can lead to platforms for the development of AHPND-resistant shrimp through selective breeding using markers mined from transcriptome analyses at different rearing conditions.

show abstract

Section: Resultssupporting

confidence: 89%

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

Boonchuen

Maralit

Jaree

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Given that Mucin glycoproteins are among the main components of the first barrier that bacteria encounter in an invasion [ 45 , 46 ], it thus suggest the downregulation of miR-211 in AHPND V. parahaemolyticus infected shrimp might be an immune defense mechanism employed by hemocytes by enhancing the expression of Mucin. Apart from these, network interaction analysis revealed some possible interaction between the AHPND-related miRNAs and HSP70, which is in tandem with a recent study which have reported that HSP70 and HSP90 are involved in the tolerance of shrimp to AHPND-causing strain of V. parahaemolyticus [ 47 ]. Collectively, these results suggest that the dysregulation of the 12 miRNAs identified following the infection of shrimps with AHPND V. parahaemolyticus was an immune defense mechanism, since this was to enhance the level of metabolism, increase the expression of immune related genes, as well as modulate cellular processes such as apoptosis.…”

Section: Discussionsupporting

confidence: 67%

Acute Hepatopancreatic Necrosis Disease (AHPND) related microRNAs in Litopenaeus vannamei infected with AHPND-causing strain of Vibrio parahemolyticus

et al. 2018

View full text Add to dashboard Cite

BackgroundAcute hepatopancreatic necrosis disease (AHPND) has emerged as a major debilitating disease that causes massive shrimp death resulting in substantial economic losses in shrimp aquaculture. Given that several diseases and infections have been associated with microRNAs (miRNAs), we conducted a comparative transcriptomic analysis using the AHPND (VA) and non-AHPND (VN) strains of Vibrio parahemolyticus to identify miRNAs potentially involved in AHPND pathogenesis in Litopenaeus vannamei.ResultsA total of 83 miRNAs (47 upregulated and 36 downregulated) were significantly differentially expressed between the VA and VN challenged groups, while 222 target genes of these miRNAs were predicted. Functional enrichment analysis revealed that the miRNAs target genes were involved in multiple biological processes including metabolic pathways, amoebiasis, Vibrio cholerae infection etc. Finally, interaction network and qPCR (Real-time Quantitative PCR) analysis of 12 potential key AHPND-related miRNAs and their predicted target genes, revealed their possible involvement in modulating several immune-related processes in the pathogenesis of AHPND.ConclusionsWe have shown using comparative transcriptomic analysis, miRNAs and their target genes that are responsive to AHPND V. parahemolyticus infection in shrimp, therefore suggesting their possible role in defense response to AHPND V. parahemolyticus infection.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4728-4) contains supplementary material, which is available to authorized users.

show abstract

“…To gain insight into the molecular bases for the observed effects, we used RNAi to assess the role of Hsp70 in protecting brine shrimp larvae against V. parahaemolyticus MO904 strain. As RNAi-mediated gene-silencing technique has been very frequently used to investigate the function of critical genes involved in the immune response ( 18 , 33 – 36 ), we have prepared dsRNA specific to Hsp70 and injected A. franciscana females in order to eliminate Hsp70 mRNA and protein from larvae released from these females. At first, we have examined the effect of Hsp70 knockdown on the survival of larvae released from females injected with either hsp70 dsRNA or gfp .…”

Section: Resultsmentioning

confidence: 99%

Phloroglucinol-Mediated Hsp70 Production in Crustaceans: Protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii

et al. 2018

View full text Add to dashboard Cite

The halophilic aquatic bacterium, Vibrio parahaemolyticus, is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND) in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 µM), protects axenic brine shrimp larvae against V. parahaemolyticus infection and induced heat shock protein 70 (Hsp70) production (twofolds or more) as compared with the control. We further demonstrated that the Vibrio-protective effect of phloroglucinol was caused by its prooxidant effect and is linked to the induction of Hsp70. In addition, RNA interference confirms that phloroglucinol-induced Hsp70 mediates the survival of brine shrimp larvae against V. parahaemolyticus infection. The study was validated in xenic Artemia model and in a Macrobrachium rosenbergii system. Pretreatment of xenic brine shrimp larvae (30 µM) and Macrobrachium larvae (5 µM) with phloroglucinol increases the survival of xenic brine shrimp and Macrobrachium larvae against subsequent V. parahaemolyticus challenge. Taken together, our study provides substantial evidence that the prooxidant activity of phloroglucinol induces Hsp70 production protecting brine shrimp, A. franciscana, and freshwater shrimp, M. rosenbergii, against the AHPND V. parahaemolyticus strain MO904. Probably, phloroglucinol treatment might become part of a holistic strategy to control AHPND in shrimp.

show abstract

HSP70 and HSP90 are involved in shrimp Penaeus vannamei tolerance to AHPND-causing strain of Vibrio parahaemolyticus after non-lethal heat shock

Cited by 81 publications

References 32 publications

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

Acute Hepatopancreatic Necrosis Disease (AHPND) related microRNAs in Litopenaeus vannamei infected with AHPND-causing strain of Vibrio parahemolyticus

Phloroglucinol-Mediated Hsp70 Production in Crustaceans: Protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii

Contact Info

Product

Resources

About