2020
DOI: 10.21203/rs.3.rs-54159/v2
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HSPA4 knockdown retarded progression and development of colorectal cancer

Abstract: Background: Colorectal cancer (CRC) is the third most common cancer worldwide and the fourth most common cause of cancer death. The heat shock 70kDa protein 4 (HSPA4) participate in progression and development of cancers. However, the cellular functions, potential molecular mechanisms of HSPA4 in CRC are still largely unknown. Methods: In this study, qRT-PCR and Western Blot were used to identify the constructed HSPA4 knockdown cell lines, which was further used to construct mouse xenotransplantation models. E… Show more

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Cited by 3 publications
(5 citation statements)
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“…In our study, HSPA4 was a hub gene and highly expressed in the TR group compared to the PR group, which was in line with the results of the external mRNA datasets. Using GSCALite, we found that HSPA4 was capable of activating the cell cycle in RC, in accordance with a previous CRC cell experiment [ 32 ]. Therefore, we assumed that HSPA4 increased the treatment sensitivity by promoting cell-cycle transitions of tumor cells, resulting in superior response to nCRT in the TR group with higher HSPA4 levels.…”
Section: Discussionsupporting
confidence: 91%
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“…In our study, HSPA4 was a hub gene and highly expressed in the TR group compared to the PR group, which was in line with the results of the external mRNA datasets. Using GSCALite, we found that HSPA4 was capable of activating the cell cycle in RC, in accordance with a previous CRC cell experiment [ 32 ]. Therefore, we assumed that HSPA4 increased the treatment sensitivity by promoting cell-cycle transitions of tumor cells, resulting in superior response to nCRT in the TR group with higher HSPA4 levels.…”
Section: Discussionsupporting
confidence: 91%
“…Futhermore, the levels of multiple HSPs including HSPA4 were significantly enhanced in hepatocellular carcinoma (HCC), CRC and head and neck cancer compared with normal tissues. In vitro experiments showed that HSPA4 modulate the proliferation and migration of HCC and CRC cells, which presumably underpins the prognostic implication of HSPA4 in CRC [ 32 ]. In CRC cell lines, HSPA4 knockdown suppressed cell proliferation and migration, and caused arrest in the G2-phase of the cell cycle along with increased levels of apoptosis by inhibiting the activation of the PI3K/Akt signaling pathway and reducing the cell cycle progression markers CCND1 and CDK6 [ 32 ].…”
Section: Discussionmentioning
confidence: 99%
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“…Intriguingly, current evidence shows that HSPA4 plays an important role in intestinal diseases [26,27]. And, high level of HSPA4 was uncovered to be elevated in colorectal cancer tissues, and downregulation of HSPA4 reduced proliferation and migration, induced G2-phase cycle arrest and apoptosis of colorectal cancer cells as well as suppressed xenograft growth though inactivating the PI3K/AKT pathway [28]. Wang et al revealed that high levels of HSPA4 in patients with RC predicted poor response to neoadjuvant concurrent chemoradiotherapy [29].…”
Section: Discussionmentioning
confidence: 99%
“…According to the sum of the correlations of these 385 genes in gastrointestinal cancer, we screened the 30 genes with the strongest correlations, which are RARS1, BTF3, HSPA4, NSA2, HNRNPA1, EIF3M, CCT4, OLA1, UIMC1, G3BP1, NACA, RSL1D1, ETF1, MRPS27, RBM22, CCT8, GEMIN5, LARS1, PA2G4, CDK7, NIFK, RSL24D1, EIF3E, THG1L, INTS13, UBE2D2, RPS23, DIMT1, RIOK2, and TTC27 respectively. Previous studies have reported that overexpression of BTF3, CCT8, CDK7, ELF3M, G3BP1, HSPA4, OLA1, and RSL1D1 can contribute to the occurrence and development of CRC (Goh et al, 2011;Zhou et al, 2019; Frontiers in Pharmacology frontiersin.org Zhou et al, 2021b;Liao et al, 2021;Zhang et al, 2021;Liu et al, 2022a;Liu et al, 2022b;Li et al, 2022), overexpression of CCT4 and PA2G4 can contribute to the occurrence and development of LIHC (Li et al, 2021a;Sun et al, 2022), overexpression of DIMT1 and HNRNPA1 can contribute to the occurrence and development of STAD (Liu et al, 2017;Zhu et al, 2022), and overexpression of EIF3E and UIMC1 can contribute to the ESCA occurrence and development (Xu et al, 2018;Yang et al, 2018). Therefore, we believe that these genes have the potential to be called diagnostic and therapeutic targets of gastrointestinal cancer.…”
Section: Discussionmentioning
confidence: 99%