Hubungan Kadar 25-Hidroksi-Vitamin D dengan HbA1c Melalui Interleukin-17 pada Anak Diabetes Melitus Tipe 1

Abstract

Latar belakang. Diabetes melitus tipe 1 (DMT1) merupakan penyakit autoimun yang ditandai oleh destruksi sel β pankreas dan berhubungan dengan aktivitas Th17. Tujuan. Mengetahui hubungan antara kadar vitamin D dengan kadar HbA1c melalui IL-17 pada anak dengan DMT1. Metode. Desain cross-sectional dan melibatkan 20 subjek DM dan 20 kontrol sehat. Kadar vitamin D diukur dengan metode EIA (ng/ mL), kadar HbA1c diukur dengan metode kromatografi menggunakan HPLC (%), IL-17 diukur dengan metode ELISA (pg/mL). Method. This study was designed as a cross-sectional study and involving 20 subjects with DM and 20 healthy subjects as control. Vitamin D level was measured by EIA method (ng/mL), while HbA1c level was measured by chromatography method using HPLC (%), and IL-17 was measured by ELISA method (pg/mL). Result. Vitamin D level in T1DM was significantly lower as compared to control group (p<0.05). HbA1c and IL-17 level was significantly higher in T1DM as compared to control group (p<0.05). Correlation study in T1DM group showed that vitamin D level was positively correlated with IL-17 level (p<0.05, R 2 = 0.566), whereas IL-17 level was negatively correlated with HbA1c level (p<0.05, R 2 = 0.489). However, path analysis showed that vitamin D insignificantly correlated with HbA1c through IL-17 (p> 0.05, R 2 = -0.267). Conclusion. We concluded that vitamin D level was lower in T1DM group, but HbA1c level and IL-17 level were higher in T1DM group. In T1DM group, vitamin D insignificantly correlated with HbA1c through IL-17. Sari Pediatri 2016; 17(6):469-77.