HUBUNGAN KEKERABATAN Shorea gysbertsiana DENGAN TIGA JENIS SHOREA PENGHASIL TENGKAWANG LAINNYA BERDASARKAN PENANDA RAPD

Sulistyawati, Purnamila; Widyatmoko, Aypbc

doi:10.20886/jpth.2018.12.2.85-94

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…There is a mass flowering occurrence once every few years, and the locals would collect the illipe nuts and dry it for a prolonged storage purpose for other uses later (Chai, 1998). According to Chai (1998) as well as Sulistyawati and Widyatmoko (2018), Shorea gysbertsiana Burck, a species from the same genus, is another name for Shorea macrophylla. However, Nurtjahjaningsih et al (2012) found that the allele frequencies of these two species are different, indicating that they are closely related but not the same.…”

Section: Introductionmentioning

confidence: 99%

Shorea macrophylla: Overview of Illipe Nut Producing Tree

Chew¹,

Chung²,

Lau³

et al. 2022

JTAS

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Shorea macrophylla is also named as ‘Engkabang’ (Iban), ‘Kawang’ (Brunei), and ‘Tengkawang’ (Indonesia). It belongs to the Dipterocarpaceae family and is categorised under the genus Shorea, which can be found in the tropical rainforests in Southeast Asia. It prefers wet habitats, such as in periodically flooded alluvium and riverbanks. The tree size of S. macrophylla is medium or large; however, some researchers in Kalimantan claim it is a small tree. The flowering and fruiting systems of most Dipterocarps, including S. macrophylla, are irregular, but there is a massive flowering event once every few years. Its genetic structure is characterised by moderate genetic diversity within species and populations, as well as high genetic differentiation within local populations. Shorea macrophylla, also known as the Light Red Meranti, is a suitable timber supply for light construction work. It produces illipe nuts that are widely used as cocoa butter replacer fat. Wildlife eats the ripe illipe nuts, which contain a high oil content with mostly beneficial unsaturated triglycerides. The fat extracted from the nut are suitable for cosmetic application as it provides a good moisturising effect. Future investigation into the illipe nuts’ composition and other potential uses should be carried out.

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Section: Introductionmentioning

confidence: 99%

Shorea macrophylla: Overview of Illipe Nut Producing Tree

Chew¹,

Chung²,

Lau³

et al. 2022

JTAS

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Molecular identification and phytochemical analysis of Kecarum (Ocimum sp.) from Bali

Naibaho,

Wirawan,

Harianja

et al. 2023

The 5th International Conference on Bioscience and Biotechnology

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Optimization of primer and polymerase chain reaction conditions to amplify COI locus for identification of Purnajiwa (Euchresta horsfieldii (Lesch.) Benn.) collected from Bedugul, Bali

Ariati

Wirawan

Sasadara

2021

IOP Conf. Ser.: Earth Environ. Sci.

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The polymerase chain reaction (PCR) has been used for molecular research to amplify DNA fragments, especially from a small amount of genetic material. PCR has been applied for numerous researches, including for plant molecular identification. A good PCR product is dependent on good amplification of the DNA segment in optimum condition. This research was conducted to optimize the primer combination, and the annealing temperature for DNA barcoding application of Balinese rare medicinal plant (Euchresta horsfieldii (Lesch.) Benn.) collected from Bedugul. COI is a suitable primer to identify unknown species to higher taxa levels and species with high phenotypic plasticity. The range of annealing temperatures was tested to amplify the combination of five COI primers: GWSF and GWSF5 for forwarding primers and GWSR, GWSR3, and GWSR5 for reverse primers. The annealing temperature was 52, 54, 56, 58, 60°C for 30 seconds. The result showed that GWSF-GWSR, GWSF-GWSR5, GWSF5-GWSR, GWSF5-GWSR5, and GWSF5-GWSR3 produced multiple bands, the double band that cannot be cut, multiple bands, faint amplification band for GWSF5-GWSR5 and GWSF5-GWSR3, respectively. Primer combination of GWSF-GWSR3 with an annealing temperature of 52°Cproduced double bands that were available to have proceeded for sequencing. In conclusion, the combination of GWSF-GWSR3 that is annealed at 52°C produced the best amplification band. The primer combinations and conditions can be used for further identification of Purnajiwa collected from Bedugul.

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HUBUNGAN KEKERABATAN Shorea gysbertsiana DENGAN TIGA JENIS SHOREA PENGHASIL TENGKAWANG LAINNYA BERDASARKAN PENANDA RAPD

Cited by 3 publications

References 13 publications

Shorea macrophylla: Overview of Illipe Nut Producing Tree

Shorea macrophylla: Overview of Illipe Nut Producing Tree

Molecular identification and phytochemical analysis of Kecarum (Ocimum sp.) from Bali

Optimization of primer and polymerase chain reaction conditions to amplify COI locus for identification of Purnajiwa (Euchresta horsfieldii (Lesch.) Benn.) collected from Bedugul, Bali

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