Huge Symbiotic Organs in Giant Scale Insects of the Genus<i>Drosicha</i>(Coccoidea: Monophlebidae) Harbor Flavobacterial and Enterobacterial Endosymbionts

Matsuura, Yu; Koga, Ryuichi; Nikoh, Naruo; Meng, Xian-Ying; Hanada, Satoshi; Fukatsu, Takema

doi:10.2108/zsj.26.448

Cited by 35 publications

(39 citation statements)

References 31 publications

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“…Moranella endobia e bacteria living inside the cells of Tremblaya symbiont in Pseudococcidae: Pseudococcinae) (von Dohlen et al, 2001;Thao et al, 2002;Ramírez-Puebla et al, 2010;McCutcheon and von Dohlen, 2011;Gatehouse et al, 2011;Gruwell et al, 2012Gruwell et al, , 2014L opez-Madrigal et al, 2013L opez-Madrigal et al, , 2015. Scale insects may also harbor the intracellular alphaproteobacterium Wolbachia sp., which is widely distributed among insects (Matsuura et al, 2009;Dhami et al, 2012). This fact suggests that the symbioses within scale insects resulted from multiple and independent infections.…”

Section: Discussionmentioning

confidence: 99%

“…Symbiotic microorganisms living in the body of so far examined scale insects are harbored, as a rule, in the cytoplasm of bacteriocytes (Walczuch, 1932;Buchner, 1965;von Dohlen et al, 2001;Szklarzewicz et al, 2006Szklarzewicz et al, , 2010Szklarzewicz et al, , 2013Ni _ znik and Szklarzewicz, 2007;Matsuura et al, 2009). The only exception was noted in Marchalina hellenica (Gennadius, 1883) (Marchalinidae) and consists in the fact that the symbionts live in the cytoplasm of cells of the midgut epithelium and are transported to the ovaries by means of temporary bacteriocytes (Buchner, 1967).…”

Section: Discussionmentioning

confidence: 99%

“…types of symbionts, their distribution in the host body and the mode of transmission to the next generation) of scale insects have not been extensively studied so far. However, it is known that in comparison with related hemipterans as aphids, whiteflies and psyllids, scale insects are characterized by an enormous diversity of symbionts (Walczuch, 1932;Buchner, 1965;Fukatsu and Nikoh, 2000;von Dohlen et al, 2001;Baumann et al, 2002;Szklarzewicz et al, 2006Szklarzewicz et al, , 2013Matsuura et al, 2009;Vashishtha et al, 2011;Gruwell et al, 2010Gruwell et al, , 2012Gruwell et al, , 2014Dhami et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

Michalik

Szklarzewicz

Kalandyk-Kołodziejczyk

et al. 2016

Arthropod Structure & Development

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

Michalik

Szklarzewicz

Kalandyk-Kołodziejczyk

et al. 2016

Arthropod Structure & Development

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“…Until now they have been reported within the families Diaspididae, Lecanodiaspididae, Coccidae, Eriococcidae, Pseudococcidae, Ortheziidae, Coelostomidiidae, and Monophlebidae (Zchori-Fein et al 2005; Gruwell et al 2007; Matsuura et al 2009; Gruwell et al 2010; Dhami et al 2012; Rosenblueth et al 2012). Flavobacteria have been considered to be the primary endosymbionts of scale insects (Gruwell et al 2007; Rosenblueth et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Genome Sequence of “Candidatus Walczuchella monophlebidarum” the Flavobacterial Endosymbiont of Llaveia axin axin (Hemiptera: Coccoidea: Monophlebidae)

Rosas-Pérez

Rosenblueth

Rincón-Rosales

et al. 2014

Genome Biology and Evolution

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Scale insects (Hemiptera: Coccoidae) constitute a very diverse group of sap-feeding insects with a large diversity of symbiotic associations with bacteria. Here, we present the complete genome sequence, metabolic reconstruction, and comparative genomics of the flavobacterial endosymbiont of the giant scale insect Llaveia axin axin. The gene repertoire of its 309,299 bp genome was similar to that of other flavobacterial insect endosymbionts though not syntenic. According to its genetic content, essential amino acid biosynthesis is likely to be the flavobacterial endosymbiont's principal contribution to the symbiotic association with its insect host. We also report the presence of a γ-proteobacterial symbiont that may be involved in waste nitrogen recycling and also has amino acid biosynthetic capabilities that may provide metabolic precursors to the flavobacterial endosymbiont. We propose “Candidatus Walczuchella monophlebidarum” as the name of the flavobacterial endosymbiont of insects from the Monophlebidae family.

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“…Fluorescence in situ hybridization was performed essentially as previously described, with some modifications (35,36). The insect samples preserved in ethanol were washed with 80% and 50% ethanol for 10 min each.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Symbionts of a Devastating Coffee Plant Pest, the Stinkbug Antestiopsis thunbergii (Hemiptera: Pentatomidae)

Matsuura

Hosokawa

Serracin

et al. 2014

Appl Environ Microbiol

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dStinkbugs of the genus Antestiopsis, so-called antestia bugs or variegated coffee bugs, are notorious pests of coffee plants in Africa. We investigated the symbiotic bacteria associated with Antestiopsis thunbergii, a major coffee plant pest in Rwanda. PCR, cloning, sequencing, and phylogenetic analysis of bacterial genes identified four distinct bacterial lineages associated with A. thunbergii: a gammaproteobacterial gut symbiont and symbionts representing the genera Sodalis, Spiroplasma, and Rickettsia. In situ hybridization showed that the gut symbiont densely occupied the lumen of midgut crypts, whereas the Sodalis symbiont, the Spiroplasma symbiont, and the Rickettsia symbiont sparsely and sporadically infected various cells and tissues. Diagnostic PCR survey of 154 A. thunbergii individuals collected at 8 localities in Rwanda revealed high infection frequencies (100% for the gut symbiont, 51.3% for the Sodalis symbiont, 52.6% for the Spiroplasma symbiont, and 24.0% for the Rickettsia symbiont). These results suggest that the gut symbiont is the primary symbiotic associate of obligate nature for A. thunbergii, whereas the Sodalis symbiont, the Spiroplasma symbiont, and the Rickettsia symbiont are the secondary symbiotic associates of facultative nature. We observed high coinfection frequencies, i.e., 7.8% of individuals with quadruple infection with all the symbionts, 32.5% with triple infections with the gut symbiont and two of the secondary symbionts, and 39.6% with double infections with the gut symbiont and any of the three secondary symbionts, which were statistically not different from the expected coinfection frequencies and probably reflected random associations. The knowledge of symbiotic microbiota in A. thunbergii will provide useful background information for controlling this devastating coffee plant pest.

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Huge Symbiotic Organs in Giant Scale Insects of the GenusDrosicha(Coccoidea: Monophlebidae) Harbor Flavobacterial and Enterobacterial Endosymbionts

Cited by 35 publications

References 31 publications

Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

Genome Sequence of “Candidatus Walczuchella monophlebidarum” the Flavobacterial Endosymbiont of Llaveia axin axin (Hemiptera: Coccoidea: Monophlebidae)

Bacterial Symbionts of a Devastating Coffee Plant Pest, the Stinkbug Antestiopsis thunbergii (Hemiptera: Pentatomidae)

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