Human Alveolar Bone Cells Interact with ProRoot and Tooth-Colored MTA

AL-Rabeah, Ebtehal; Perinpanayagam, Hiran; MacFarland, Don

doi:10.1016/j.joen.2006.03.019

Cited by 89 publications

(75 citation statements)

References 15 publications

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“…Although the effect of MTA on MC3T3-E1 cells was minimal, a significant difference between the groups was found on day 3. Our results were consistent with previously reports that MTA enables proliferation of various cell types [4][5][6][7][8] , and the excellent biocompatibility of MTA was confirmed.…”

Section: Discussionsupporting

confidence: 93%

“…Thus, MTA has been widely used in endodontic treatments, including direct pulp capping, root-end filling, perforation repair, and apexification [1][2][3] . MTA bioactivity involves the ability to enhance cell proliferation, migration, and mineralization in various cell types [4][5][6][7][8][9] . MTA contains primarily calcium silicate, which is converted into calcium hydroxide upon contact with tissue fluid.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Yasukawa

Hayashi

Tanabe

et al. 2017

Dental Materials Journal

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Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-E1 cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Yasukawa

Hayashi

Tanabe

et al. 2017

Dental Materials Journal

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“…The major difference between gray and white MTA (WMTA) is the concentration of iron oxide (FeO). The amount of FeO is extremely reduced in the WMTA (3), while the biological properties of both types of cement are comparable (4)(5).…”

Section: Introductionmentioning

confidence: 99%

L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

Miranda¹,

Fidel

Boller

2009

Braz. Dent. J.

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This study compared the cytotoxicity of an experimental epoxy-resin and calcium hydroxide-based cement (MBPc), gray mineral trioxide aggregate (MTA) and white mineral trioxide aggregate (WMTA) using the agar overlay method with neutral red dye. L929 cells were seeded into 6-well culture plates where 48-h set test materials were placed on the agar overlay, in triplicate. Teflon and natural rubber served as negative and positive controls. After an incubation period of 24 h at 37ºC in a humidified atmosphere of 5% CO 2 in air, a discolored area around the samples and the positive controls could be observed and measured per quadrant. The mean values were compared and converted into grades to classify the results according to the table of cytotoxicity grades according to the Standard Operating Procedures (SOP) of the Oswaldo Cruz Foundation, Brazil. The nonviable cell areas and the morphological changes in the cells were observed with an inverted microscope. The results showed grade 1 (slight) for the two types of MTA (p>0.05) and grade 2 (mild) for the MBPc (p<0.001). All samples met the requirements of the test as none of the cultures showed reactivity higher than grade 2.

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“…It can also promote the regeneration of alveolar bone 39 and cementum 40, 41. This case made use of MTA to seal the root canal orifice of the additional root to prevent infections and blood flow to the main root canal during the periodontal surgery.…”

Section: Discussionmentioning

confidence: 99%

Papillary reconstruction and guided tissue regeneration for combined periodontal–endodontic lesions caused by palatogingival groove and additional root: a case report

Miao

Chen

Otgonbayar

et al. 2015

Clinical Case Reports

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Key Clinical MessageWe described a combined periodontal–endodontic lesion, which was caused by a palatogingival groove and an additional root. An interdisciplinary approach involving endodontic therapy, mineral trioxide aggregate (MTA) filling, root resection, guided tissue regeneration, and papillary reconstruction was used for the case. The tooth presents morphologically and functionally normal except tooth discoloration caused by MTA.

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Human Alveolar Bone Cells Interact with ProRoot and Tooth-Colored MTA

Cited by 89 publications

References 15 publications

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

Papillary reconstruction and guided tissue regeneration for combined periodontal–endodontic lesions caused by palatogingival groove and additional root: a case report

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