Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Abstract

Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-E1 cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.

Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material

“…This is attributed to the suitable pH and calcium ions levels in NSY-224(60). It has been reported that calcium ions stabilize or enhance the viability of dental pulp cells [ 44 ], periodontal ligament cells [ 45 ], and odontoblasts [ 46 ]. Additionally, pH is also an important factor that influences the wound healing process by changing throughout the process [ 47 , 48 , 49 ].…”

Microporous polysaccharide multilayer coated BCP composite scaffolds with immobilised calcitriol promote osteoporotic bone regeneration both in vitro and in vivo

“…By contrast, Chatopadhyay et al showed that inactivation of CaSR decreased rat calvarial osteoblast proliferation and differentiation 55. Mechanistically, the activation of CaSR directly stimulated RUNX-2 expression, which is referred to as the master switch of osteogenic differentiation due to its targeting of the transcription of osteoblast-related genes such as those encoding osteocalcin (OCN), type I collagen, and ALP 56-58. Among these factors, OCN and type I collagen are the main constituents of bone extracellular matrix (ECM) and ALP is responsible for mineralisation of the ECM 58.…”

The role of Ca2+/Calcineurin/NFAT signalling pathway in osteoblastogenesis

“…The CaSR belonging to the G protein‐coupled receptors can sense the extracellular Ca 2+ concentration and instantaneously mobilize intracellular Ca 2+ flux 68 . Previous studies have shown that CaSR signals mediate the osteogenic differentiation of BMSCs in vitro and bone formation in vivo, and CaSR agonists can promote the proliferation, differentiation and matrix mineralization of osteoblasts 41,69,70 . In addition, Ca 2+ influx caused by mechanically sensitive channels also promotes osteogenic differentiation 71 ; Orai1 gene knockout leads to impaired osteoblast differentiation and mineralization 43 …”