Human amnion-derived mesenchymal stem cells alleviate lung injury induced by white smoke inhalation in rats

Abstract

BackgroundWhite smoke inhalation (WSI) is an uncommon but potentially deadly cause of acute lung injury and acute respiratory distress syndrome for which no effective pharmaceutical treatment has been developed. This study aimed to determine the protective effects of human amnion-derived mesenchymal stem cells (hAMSCs) against WSI-induced lung injury in rats.MethodshAMSCs were injected into rats via the tail vein 4 h after WSI. At 1, 3, 7, 14, and 28 days after cell injection, hAMSCs labeled with PKH26 in lung, heart, liver, and kidney tissues were observed by fluorescence microscopy. The lung injury score was determined by hematoxylin and eosin staining. Lung fibrosis was assessed by Masson’s trichrome staining. The computed tomography (CT) score was assessed by CT scanning. The wet/dry weight ratio was calculated. The levels of interleukin (IL)-1β, IL-6, and IL-10 were determined by enzyme-linked immunosorbent assays. The expression of surfactant protein (SP)-A, SP-C, and SP-D was measured by Western blotting.ResultsThe injected hAMSCs were primarily distributed in the lung tissues in WSI-induced rats. Compared with the model and phosphate-buffered saline (PBS) group, hAMSC treatment led to reduced lung injury, lung fibrosis, CT score, and inflammation levels in WSI-induced mice. hAMSC treatment also resulted in increased cell retention in the lung, partial pressure of oxygen (PaO2), and PaO2/fraction of inspired oxygen (FiO2) levels, and pulmonary SP-A, SP-C, and SP-D expression compared with that in the model and PBS group.ConclusionshAMSCs are a potential cell-based therapy for WSI-induced lung injury.

Human Adipose-Derived Mesenchymal Stem Cells Modify Lung Immunity and Improve Antibacterial Defense in Pneumosepsis Caused byKlebsiella pneumoniae

“…With reference to enhanced expression of genes implicated in fibrotic responses in animals infused with ASCs, it should be noted that the current model is acute and fibrosis cannot be detected at histological level. In more chronic settings, infusion of MSCs have been shown to exert antifibrotic effects in the lungs in several experimental settings, including lung fibrosis induced by bleomycin , white smoke , silica , and chronic allergic airway inflammation . These preclinical data led several investigators to suggest infusion of MSCs as a potential novel therapeutic for lung fibrosis in patients .…”

Emerging therapies for smoke inhalation injury: a review

“…Western blot was used to analyze surfactant protein levels and Enzyme Linked Immunosorbent Assay (ELISA) was used to detect inflammatory cytokines in BALF, both of which play a role in inflammation and pulmonary immune response. The results demonstrated overall pulmonary improvement in rats treated with hAMSC [18]. The labeled hAMSC were observed primarily in lung tissue after smoke inhalation injury, and the treatment group had significant improvement in CT pathology and histopathological score 28 days after inhalation injury, as well as decreased wet-to-dry ratio, indicating improvement in pulmonary edema [18].…”

“…Cui et al evaluated the pulmonary effect of amnion mesenchymal stem cells administered after smoke inhalation injury. Human amnion mesenchymal stem cells (hAMSC) were injected in rats via the tail vein after exposure to white smoke [18]. The degree of inhalation injury was subsequently analyzed using CT scan, wet-todry ratio, histopathology, and evaluation of blood gases.…”

The Patenting of Products and Processes Used for the Treatment of Smoke Inhalation

“…At the experimental level, the use of mesenchymal stem cells derived from human amnion (hAMSCs) alleviated white smoke-induced lung injury [21]. It is likely that in the future this resource will contribute to the best clinical outcome for victims of this type of injury.…”