Human auricolar myiasis caused by <i>Wohlfartia magnifica</i> (Schiner) (Diptera: Sarcophagidae): first case found in Sardinia

Panu, Francesco; G, Cabras; Contini, Carlo; Onnis, Del Rio Pèrez

doi:10.1258/0022215001905814

Cited by 34 publications

(23 citation statements)

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“…The most important species causing aural myiasis are C. hominivorax (232,245,355), W. magnifica (34,100,180,264,341,350), C. bezziana (1,8,134,289), C. megacephala (203), Sarcophaga (6,214,347,353,360), and Parasarcophaga crassipalpis (240).…”

Section: Cavitary Myiasismentioning

confidence: 99%

Myiasis

2012

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SUMMARY Myiasis is defined as the infestation of live vertebrates (humans and/or animals) with dipterous larvae. In mammals (including humans), dipterous larvae can feed on the host's living or dead tissue, liquid body substance, or ingested food and cause a broad range of infestations depending on the body location and the relationship of the larvae with the host. In this review, we deeply discuss myiasis as a worldwide infestation with different agents and with its broad scenario of clinical manifestations as well as diagnosis techniques and treatment.

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Section: Cavitary Myiasismentioning

confidence: 99%

Myiasis

2012

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“…O mais comum é o aparecimento de miíases em pacientes com lesões necróticas cavitárias, como colesteatomas na orelha média, tumores ou doenças úlcero-granulomatosas nasais (leishmaniose, hanseníase etc.) tumores orais, anais ou vaginais e oftálmicos, assim como lesões da pele 7,9,20,19,15,10 . Essas miíases (facultativas ou secundárias) são provocadas por larvas de moscas necrobiontófogas (se alimentam de tecido morto), sendo a mais comum em nosso meio a Cochliomya macellaria e a Phaenicia cuprina (Lucilia).…”

Section: Quanto àS Miíasesunclassified

Tratamento da miíase humana cavitária com ivermectina oral

Ribeiro¹,

Pereira²,

Alves³

et al. 2001

Rev. Bras. Otorrinolaringol.

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Treatment of human cavitary myiasis with oral ivermectinResumo / Summary ORIGINAL ARTICLE Introdução: Miíase humana é uma ocorrência relativamente comum e, no mínimo, constrangedora, quer seja para os pacientes ou para o médico que o atende. É bem mais freqüente nos países subdesenvolvidos e tropicais, mas há casos descritos em todas as regiões do Planeta. Normalmente, afeta pacientes doentes, idosos e deficientes mentais, mas pode ocorrer em pacientes tróficos e saudáveis. As larvas depositam seus ovos em tecidos doentes e necróticos, mas podem fazê-lo em zonas do corpo aparentemente sãs. Seu tratamento consiste na catação das larvas, um processo desagradável e doloroso, muitas vezes impossÍvel em regiões cavitárias. Alguns produtos foram usados no sentido de facilitar esse procedimento, sem grandes resultados. Forma de estudo: Prospectivo randomizado. Método: Neste trabalho, foi feito, em sete pacientes, o tratamento da miíase cavitária humana com ivermectina oral (até 300 µg/kg), medicamento já usado para o tratamento de outras doenças. Os pacientes foram submetidos a provas de função hepática e renal pré e pós-tratamento, e acompanhados clinicamente. Resultados: Todos os pacientes tiveram as larvas eliminadas; e nenhuma anormalidade, nos exames.

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“…Females may deposit first instar larvae directly onto the vertebrate host at predisposing sites (causing secondary myiasis), carcasses, decomposing organic matter, and dung (Panu et al 2000). Genus Oxysarcodexia is widely distributed in the Neotropical regions (Lopes 1946), and in Brazil, O. thornax (Walker) is found in both urban and rural areas of several states.…”

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confidence: 99%

Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva

Cuervo¹,

Mesquita-Rodrigues²,

d’Avila-Levy³

et al. 2008

Mem. Inst. Oswaldo Cruz

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We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases.Keywords: Oxysarcodexia thornax -Sarcophagidae -chymotrypsin-like serine proteasesThe genus Oxysarcodexia belongs to the Sarcophagidae family (flesh flies) whose major biological feature is the ovo-larvipary (Pape 1996). Females may deposit first instar larvae directly onto the vertebrate host at predisposing sites (causing secondary myiasis), carcasses, decomposing organic matter, and dung (Panu et al. 2000). Genus Oxysarcodexia is widely distributed in the Neotropical regions (Lopes 1946), and in Brazil, O. thornax (Walker) is found in both urban and rural areas of several states. This species is abundant in the state of Rio de Janeiro, occurring preferentially in the summer season, with the population peak between January and February (Oliveira et al. 2002).In biological systems, proteases may carry out (i) a regulatory function by activation or inactivation of specific proteins via selective proteolysis, and (ii) a nonspecific proteolytic function involved in general protein hydrolysis. Such proteolytic mechanisms are highly regulated and are involved in a variety of physiologic processes, such as programmed cell death, stress responses (heat shock and anoxia), skeletal muscle atrophy, cellcell recognition, signal transduction and learning, morphogenesis, and photoreceptor light adaptation (Mykles 1998). Different classes of proteases such as cysteine proteases, metalloproteases and serino proteases have been described both in adults and larvae of several species of the Diptera (Han et al. 1997, Rosenfeld & Vanderberg 1998, Cho et al. 1999, Noriega et al. 2002, Vierstraete et al. 2003, Okuda et al. 2005, Fazito do Vale et al. 2007, Pires et al. 2007, Rodrigues et al. 2007). Despite the existence of information about morphology, taxonomy and ecology of this genus, as far as we know, no data has been published on the biochemical traits of O. thornax. In the present study, the proteolytic profile of O. thornax first instar larvae was investigated using zymographic analysis. O. thornax adults were collected as previously described (Oliveira et al. 2002) and females were kept in a 50 ml plastic vial for 24 h at room temperature. Following death of the females, abundant live first instar larvae (L1) were released and immediately collected. Larvae were ...

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Human auricolar myiasis caused by Wohlfartia magnifica (Schiner) (Diptera: Sarcophagidae): first case found in Sardinia

Cited by 34 publications

References 2 publications

Myiasis

Myiasis

Tratamento da miíase humana cavitária com ivermectina oral

Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva

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