Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds

Sanapalli, Bharat Kumar Reddy; Yele, Vidyasrilekha; Singh, Mantosh Kumar; Thumbooru, Shilpa.N.; Parvathaneni, Madhukiran; Karri, Veera Venkata Satyanarayana Reddy

doi:10.1016/j.biopha.2023.114540

Cited by 16 publications

(4 citation statements)

References 75 publications

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“…Similarly, 10-week treatment of HFD-fed mice with the modified HD5 1–9 fragment, D3, improved glucose clearance rate comparable to those of normal chow-fed mice, indicating that the HD5 1–9 fragment, D3, may improve obesity-induced insulin resistance [ 37 ]. Similarly, hBD2 was found to regulate diabetic wound healing in rats, as hBD2-loaded Poly-lactic-co-glycolic acid (PLGA) nanoparticles accelerated healing, which was associated with decreased Mmp9 and TNFa expression [ 54 ]. However, our results provide evidence for the first time that hBD2, comparable to HD5 1–9 , also modulates glucoregulatory capacity in WSDF-fed mice and thus could be an interesting therapeutic approach for diet-induced disturbances in glucose metabolism.…”

Section: Discussionmentioning

confidence: 99%

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Filipe Rosa,

Rings,

Stolzer

et al. 2023

IJMS

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Obesity and metabolic comorbidities are associated with gut permeability. While high-fructose and Western-style diet (WSD) disrupt intestinal barrier function, oral administration of human α-defensin 5 (HD5) and β-defensin 2 (hBD2) is believed to improve intestinal integrity and metabolic disorders. Eighty-four male C57BL/6J mice were fed a WSD or a control diet (CD) ± fructose (F) for 18 weeks. In week 13, mice were randomly divided into three intervention groups, receiving defensin fragment HD51–9, full-length hBD2, or bovine serum albumin (BSA)-control for six weeks. Subsequently, parameters of hepatic steatosis, glucose metabolism, and gut barrier function were assessed. WSDF increased body weight and hepatic steatosis (p < 0.01) compared to CD-fed mice, whereas peptide intervention decreased liver fat (p < 0.05) and number of hepatic lipid droplets (p < 0.01) compared to BSA-control. In addition, both peptides attenuated glucose intolerance by reducing blood glucose curves in WSDF-fed mice. Evaluation of gut barrier function revealed that HD51–9 and hBD2 improve intestinal integrity by upregulating tight junction and mucin expression. Moreover, peptide treatment restored ileal host defense peptides (HDP) expression, likely by modulating the Wnt, Myd88, p38, and Jak/STAT pathways. These findings strongly suggest that α- and β-defensin treatment improve hepatic steatosis, glucose metabolism, and gut barrier function.

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Section: Discussionmentioning

confidence: 99%

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Filipe Rosa,

Rings,

Stolzer

et al. 2023

IJMS

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“…These findings correlate with the varying pore sizes of the microspheres cross-linked with GA, GE, and EDC/NHS, aligning with the previously mentioned SEM observations. These findings indicate that increasing the cross-linking within hydrogels stabilizes the biopolymer framework, which in turn reduces the intermolecular gap and pore size . Consequently, this reduction in pore size leads to a decreased water storage and absorption capacity of the hydrogel.…”

Section: Resultsmentioning

confidence: 91%

“…These findings indicate that increasing the cross-linking within hydrogels stabilizes the biopolymer framework, which in turn reduces the intermolecular gap and pore size. 36 Consequently, this reduction in pore size leads to a decreased water storage and absorption capacity of the hydrogel.…”

Section: Physicochemical Properties Of Eyeball-shaped Gel Microspheresmentioning

confidence: 99%

Construction of Dome-Shaped 3D Corneal Epithelial Tissue Models Based on Eyeball-Shaped Gel Microspheres

Wang,

Yang,

Yang

et al. 2024

ACS Appl. Mater. Interfaces

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“…Its hydrophobic nature does not offer itself a suitable platform for cell adherence, but it can be functionalized or associated with other biopolymeric materials or particles/molecules to improve its biofunctionality. The glass transition temperature (Tg) of these polymers is around 37 • C. Also, PLGA has successfully been used for decades in several skin and tissue engineering applications as nanoparticles [122,123], microparticles [124], electrospun co-axial fibers [125], bilayer electrospun membranes [126], nanosuspensions [127], thermogel dressing [128], hydrogels [129], microspheres [130], composite biofilms [131] and nanocomposites scaffolds [132].…”

Section: Poly(lactide-co-glycolide) Plgamentioning

confidence: 99%

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

Elango,

Zamora-Ledezma,

Maté-Sánchez de Val

2023

J. Compos. Sci.

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Modern research has evolved several approaches toward skin regeneration and one of the novel concerns is the use of polymer-based systems due to their excellent beneficial properties to the skin. Several polymers, such as cellulose, hyaluronan, alginate, chitosan, collagen, fibrin and fibroin, have been tested and have proven the benefits for skin regeneration, and most of them are derived from either polysaccharide- or protein-based materials. In order to understand the mode of action, several researchers investigated the cell–matrix interaction and possible signaling mechanism in skin regeneration. Not only the signaling mechanism but also the mode of cell communication determines the application of polysaccharide- and protein-based polymers in practice. Based on the above significance, this review disclosed the recent findings to compile a possible method of communication between cells and polymers derived from polysaccharide-based (such as cellulose, hyaluronan, chitosan, alginate, agar, and xanthan gum) and protein-based (such as collagen, gelatin, fibrin, and silk fibroin) materials along with other polymers, such as poly(vinyl alcohol), polyglycolide or poly(glycolic acid), or poly(lactic acid) in skin regeneration. Accordingly, this review addresses the fundamental concept of cell–matrix communication, which helps us to understand the basis of the polymer’s functions in the biomedical field.

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Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds

Cited by 16 publications

References 75 publications

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Construction of Dome-Shaped 3D Corneal Epithelial Tissue Models Based on Eyeball-Shaped Gel Microspheres

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

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