Human blood group typing based on digital photographs of RBC agglutination process

Doubrovski, V. A.; Dolmashkin, A. A.

doi:10.1134/s0030400x10080187

Cited by 10 publications

(5 citation statements)

References 5 publications

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“…There are a large number of methods for BG typing [14], which can be classified into classical, such as tube or slide tests, or modern methods [15], such as microplate or gel centrifugation. Some other modernday methods utilize nucleic acid amplification, ultrasound back scattering [16], hybrid methods-laser radiation followed by digital imaging [17,18]-and polymerized chain reaction with sequence-specific priming (PCR-SSP) strategies for human BG typing with more accuracy [19]. Both classical and advanced methods aim at determination of rare or weak alleles of BG, but there is a compromise between sensitivity, analysis time and cost of that particular test.…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Erythrocytes with QCMs—ABO Blood Group Typing

Latif,

Seifner,

Dickert

2023

Sensors

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Blood transfusion, as well as organ transplantation, is only possible after prior blood group (BG) typing and crossmatching. The most important blood group system is that of Landsteiner’s ABO classification based on antigen presence on the erythrocyte surfaces. A mass sensitive QCM (quartz crystal microbalance) sensor for BG typing has been developed by utilizing molecular imprinting technology. Polyvinylpyrrolidone (crosslinked with N,N-methylenebisacrylamide) is a favorable coating that was imprinted with erythrocytes of different blood groups. In total, 10 MHz quartz sheets with two resonators, one for MIP (molecularly imprinted polymer) and the other for NIP (non-imprinted polymer) were fabricated and later used for mass-sensitive measurements. The structure of erythrocyte imprints resembles a donut, as identified by AFM (atomic force microscope). All the erythrocytes of the ABO system were chosen as templates and the responses to these selective coatings were evaluated against all blood groups. Each blood group can be characterized by the pattern of responses in an unambiguous way. The results for blood group O are remarkable given that all types of erythrocytes give nearly the same result. This can be easily understood as blood group O does not possess neither antigen A nor antigen B. The responses can be roughly related to the number of respective antigens on the erythrocyte surface. The imprints generate hollows, which are used for reversible recognition of the erythrocytes. This procedure is based on molecular recognition (based on supramolecular strategies), which results from size, shape and enthalpic interactions between host and guest molecules.

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Section: Introductionmentioning

confidence: 99%

Selective Detection of Erythrocytes with QCMs—ABO Blood Group Typing

Latif,

Seifner,

Dickert

2023

Sensors

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“…We presented this method for the first time in analog form in [9] and described it in detail in [16]. In [17] it was proposed for the first time to use a digital camera for AOM of blood typing, therefore to use the digital processing of the experimental results. Then the various options of AOM of blood typing were examined based on various physical principles using digital camera, e.g.…”

Section: Introductionmentioning

confidence: 99%

Digital Processing Techniques Influence on Resolution of Acousto-Optical Blood Typing Method

Doubrovski

Markov

2022

Optics and Spectroscopy

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The various digital techniques of photo images processing influence upon the resolution of the acousto-optical method (AOM) for instrumental blood typing according to the AB0 system has been studied. Experiments with blood samples were carried out using monoclonal antibodies (tsoliclones) of anti-A and anti-B types, as well as without them ("clean" sample) when the mixture was exposing simultaneously by a standing ultrasonic wave. The experiment was recorded by a digital camera. Different variants of statistical calculations have been proposed to process the photo images of the recorded phenomena, the results were compared with the traditional photometric approach. It is shown that the statistical processing of experimental results gives a higher resolution in blood group determination and, consequently, a higher reliability of its typing. A comparison of different variants for statistical photo frames processing was carried out to study the possibility to increase the resolution of AOM in blood typing. Keywords: acousto-optical blood typing method, monoclonal antibodies, digital processing techniques, blood typing, high resolution.

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“…It was for the first time when the authors were involved into the problem of automatic device creation for blood typing based on the use of a probing optical beam and stimulating ultrasonic radiation simultaneously in 1986 [13]. In the further studies and developments this method was carried out in analog [18][19][20] or digital forms [21][22][23][24][25][26][27][28][29] and in the whole it was named as "acousto-optical method for blood group determination".…”

Section: Introductionmentioning

confidence: 99%