Human bone marrow stroma‐dependent cell line MOLP‐5 derived from a patient in leukaemic phase of multiple myeloma

Abstract

Summary. The novel multiple myeloma (MM) cell line MOLP-5 and its homologous sister cell line B407, a lymphoblastoid cell line (LCL), were established from the peripheral blood of a 71-year-old Japanese patient with Bence-Jones k-type multiple myeloma (stage IIIB with hyperammonaemia and hypercalcaemia). The growth of MOLP-5 cells is constitutively dependent on bone marrow stroma (BST) cells; none of the cytokines tested nor the culture supernatant of the bone marrow stroma cells could support the growth of MOLP-5. Wright±Giemsa-stained MOLP-5 cells showed typical plasma cell morphology with abundant cytoplasm and one to three nuclei. The immunoprofile of MOLP-5 corresponds to that seen typically in primary MM cells: positive for cytoplasmic immunoglobulin (Ig) k light chain, CD28, CD29, CD38, CD40, CD44, CD49d, CD54, CD56, CD58, CD71, CD138 and PCA-1; the cells were negative for surface Ig and various other B-cell, T-cell and myelomonocyte-associated immunomarkers. Interleukin 6 (IL-6) receptor mRNA was found in the reverse transcriptase polymerase chain reaction (RT-PCR) analysis. IL-6 and IL-10 could induce cellular proliferation in shortterm induction experiments. IL-6 or IL-10 production was not detected by specific enzyme-linked immunoabsorbent assay (ELISA). MOLP-5 cells expressed parathyroid hormone-related protein (PTHrP) at the mRNA level. Cytogenetic analysis showed the typical t(11; 14) chromosome abnormality. The novel MOLP-5 cell line together with the B407 B-LCL sister line will be useful model systems in the investigation of the biology of MM.

Niche-Based Screening in Multiple Myeloma Identifies a Kinesin-5 Inhibitor with Improved Selectivity over Hematopoietic Progenitors

“…Co-culture with BMSCs maintained the viability of CD138(+) primary MM cells >5-fold better than culture media alone (Figure 1B) confirming previously observed stroma-dependence of primary MM(Zlei et al, 2007). To recapitulate this behavior in a cell-line amenable to high-throughput screening, the stroma-dependent MM cell-line MOLP5(Matsuo et al, 2000), stably expressing GFP, was similarly grown alone or in co-culture with stroma in 384-well plates. A similar ∼7-fold increase in GFP(+) MOLP5 cells was observed with stromal co-culture (Figure 1B) using image-based quantification, making this an attractive physiologically relevant high-throughput assay to screen for small-molecule inhibitors of MM within its niche.…”

Anaplastic plasmacytoma with malignant pleural effusion lacking evidence of monoclonal gammopathy

“…However, typical immunomarkers for lymphoma and monotypic immunoglobulins for plasmacytoma (determined immunohistochemically and in serum and urine) were also absent. The main indications of plasmacytic differentiation were immunostaining for the plasma-cell markers, CD138, MUM1, and especially VS38c [9,14] and the presence of rough endoplasmic reticulum cisternae in cells with eccentric nuclei seen by electron microscopy. Recently, unusual acquired immunodeficiency syndrome (AIDS)-related lymphomas with plasmacytic features and massive malignant pleural effusion have been described -plasmablastic lymphoma of the lung [8] and AIDS-related primary effusion lymphoma [1].…”

Lenalidomide in combination with an activin A-neutralizing antibody: preclinical rationale for a novel anti-myeloma strategy

“…The human bone marrow (BM) stroma-dependent MM cell line (MOLP5) was provided by Dr Y Matsuo (Okayama, Japan). 8 BM aspirates from MM patients were obtained following approval from the Massachusetts General Hospital Institutional Review Board. After mononuclear cell separation, MM cells were purified by positive CD138 (Syndecan-1) Micro Bead selection as previously described.…”