Human burst-forming units-erythroid need direct interaction with stem cell factor for further development

Dai, CH; Krantz, SB; Zsebo, KM

doi:10.1182/blood.v78.10.2493.bloodjournal78102493

Cited by 8 publications

(8 citation statements)

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“…However, upon exposure to FK228, the CD36 + GPA ) cells were induced to form a significant number of erythroid colonies in the presence of IL-3. This further suggests that, although IL-3 responsiveness of early erythroid precursors decreases during differentiation (Dai et al, 1991), it can be restored and enhanced by inhibition of HDAC activity during the early stages of erythroid differentiation. In general, sequence-specific DNA-binding repressors and HDAC form a repressor complex that suppresses the transcription of target genes (Pazin & Kadonaga, 1997;Kramer et al, 2001;Redner & Liu, 2005).…”

Section: Role Of Histone Deacetylases In Erythropoiesismentioning

confidence: 91%

Pleiotropic role of histone deacetylases in the regulation of human adult erythropoiesis

et al. 2006

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Summary Histone acetylation and deacetylation play fundamental roles in transcriptional regulation. We investigated the role of histone deacetylases (HDACs) in human adult haematopoiesis, using the structurally distinct HDAC inhibitors FK228 (depsipeptide) and Trichostatin A. When CD34+ cells were cultured with interleukin (IL)‐3 or stem cell factor (SCF) + IL‐3, FK228 (0·5 ng/ml) specifically enhanced the generation of immature erythroid cells with a CD36+ glycophorin A (GPA)low phenotype. In semisolid cultures, FK228 promoted the formation of erythroid colonies by CD34+ cells with IL‐3 and SCF + IL‐3. Furthermore, upon exposure to FK228, CD34+ cell‐derived CD36+GPA− cells were induced to form erythroid colonies with IL‐3 alone. Conversely, FK228 inhibited the generation of CD36+GPAhigh relatively mature erythroid cells from CD34+ cells in the presence of erythropoietin (EPO) and SCF + EPO. FK228 suppressed the EPO‐mediated survival of CD36+GPAlow/‐and CD36+GPAhigh cells and induced their apoptosis. Similar effects were observed for trichostatin A in the generation of erythroid cells in IL‐3‐ and EPO‐containing cultures. These data suggest that HDACs negatively regulate the IL‐3‐mediated growth of early erythroid precursors by suppressing their responsiveness to IL‐3, while playing an important role in EPO‐mediated differentiation and survival of erythroid precursors. Our data revealed that HDACs have diverse functions in human adult erythropoiesis.

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Section: Role Of Histone Deacetylases In Erythropoiesismentioning

confidence: 91%

Pleiotropic role of histone deacetylases in the regulation of human adult erythropoiesis

et al. 2006

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“…However, SCF did not enhance K562 haemoglobin production, with IL-3 being more effective in this role. IL-3 is important, for the early stages of haemoglobin production on early human BFU-E progenitors need direct interaction with SCF for further erythroid development (Dai et al, 1991). Therefore one can conclude that the erythropoietic development of these cells requires both factors.…”

Section: Clonogenic Assays and Haemoglobin Detectionmentioning

confidence: 99%

A novel approach to investigating the erythroid lineage, using both receptor analysis and haemoglobin detection

McGuckin¹,

Liu²,

Gordon‐Smith³

et al. 1996

Br J Haematol

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Progenitor cell failure in the erythroid lineage is a particular problem in bone marrow failure. To provide insight into early erythopoietic development we used sensitive techniques to examine the effects of SCF, IL-3 and MIP-1 alpha on two developmentally arrested progenitor cell lines, HEL and K562. Quantitative flowcytometric analysis showed that both expressed receptors (SCF > MIP-1 alpha > IL-3). Qualitative analysis revealed HEL cells expressed more receptors than K562 cells. Clonogenic assays with sensitive haemoglobin detection showed that SCF and IL-3 did not support HEL development and reduced haemoglobin production. MIP-1 alpha reduced partially developed HEL colonies and haemoglobin in developed colonies. SCF increased development, but not haemoglobin in K562 cells, with IL-3 being more effective in both. MIP-1 alpha increased the proportion of well-developed K562 colonies but not haemoglobin. This suggests SCF, IL-3 and MIP-1 alpha all have a role to play in early erythroid cellular development, with differing actions depending on the stage of development.

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“…Actually, the previous techniques combined both complex negative selections and culture stages before obtaining satisfactory red cell enrichments. For example, the puri®cation of day-6 erythroid colony-forming cells (ECFC) used in several studies (Fibach et al, 1989;Sawada et al, 1989;Dai et al, 1991Dai et al, , 1998Wickrema et al, 1992;Giampaolo et al, 1994;Muta et al, 1995) consisted of a multistep procedure comprising, after Ficoll-Hypaque separation, sheep erythrocyte rosetting of lymphoid T cells, overnight adherence for depletion of monocytes, followed by the use of a mixture of four monoclonal antibodies to remove granulocytes, B lymphocytes, NK cells and residual T cells. Thereafter, cells were cultured in methylcellulose in the presence of 30% FCS and Epo for 6 d, then collected from the culture and submitted again to adhesion and Ficoll-Hypaque separation before secondary culture in the presence of FCS, human AB serum and Epo.…”

Section: Discussionmentioning

confidence: 99%

“…However, the commitment of stem cells to both early and late erythroid progenitors (BFU-Es and CFU-Es) appears to be a stochastic process independent of the presence of Epo (Ogawa, 1993;Papayannopoulou et al, 1993;Wu et al, 1995b;Socolovsky et al, 1997), and red cell terminal differentiation itself can be induced in vitro independently of the presence of Epo (Sui et al, 1996;Goldsmith et al, 1998). A variety of cytokines have been reported to stimulate primitive erythropoiesis, including stem cell factor (SCF) (Dai et al, 1991;Papayannopoulou et al, 1993;Muta et al, 1995;Wu et al, 1995aWu et al, , 1997Broudy, 1997) and interleukin-6 (IL-6) (Sui et al, 1996). Other synergistically acting cytokines such as IL-1 and IL-3 also seem to play a role in the generation and/or ampli®cation of erythroid progenitors (Brugger et al, 1993;Papayannopoulou et al, 1993).…”

mentioning

confidence: 99%

Purification, amplification and characterization of a population of human erythroid progenitors

Freyssinier¹,

Lecoq-Lafon

Amsellem³

et al. 1999

Br J Haematol

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In humans, studies of the erythroid cell lineage are hampered by difficulties in obtaining sufficient numbers of erythroid progenitors. In fact, these progenitors in bone marrow or peripheral blood are scarce and no specific antibodies are available. We describe a new method which allows proliferation in liquid culture of large numbers of pure normal human erythroid progenitors. CD34+ cells were cultured for 7 d in serum-free conditions with the cytokine mixture interleukin (IL)-3/IL-6/stem cell factor (SCF). This resulted in cell expansion and the appearance of a high proportion of CD36+ cells which were purified on day 7. Methylcellulose clones from these cells were composed of 96.6% late BFU-E and 3.4% CFU-GM. These CD36+ cells could be recultured with the same cytokine mixture plus or minus erythropoietin (Epo) for a further 2-7 d. In both conditions further amplification of CD36+ cells was observed, but Epo induced a more dramatic cell expansion. Glycophorin-positive mature cells appeared only in the presence of Epo, and terminal red cell differentiation was observed after 7 d of secondary culture. Cells obtained from adult CD34+ progenitors mostly contained adult haemoglobin, whereas cord blood-derived cells contained equal proportions of adult and fetal haemoglobin. Activation of STAT5 and tyrosine phosphorylation of the Epo receptor and JAK2 were observed after Epo stimulation of these cells. This new method represents a straightforward alternative to the procedures previously described for the purification of normal erythroid progenitors and is useful in the study of erythropoietic regulation.

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Human burst-forming units-erythroid need direct interaction with stem cell factor for further development

Cited by 8 publications

References 0 publications

Pleiotropic role of histone deacetylases in the regulation of human adult erythropoiesis

Pleiotropic role of histone deacetylases in the regulation of human adult erythropoiesis

A novel approach to investigating the erythroid lineage, using both receptor analysis and haemoglobin detection

Purification, amplification and characterization of a population of human erythroid progenitors

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