Human Coronavirus Species and their correlation as co-infection detected by Fast Real-Time RT-PCR

Salah, Hala A.; Aufi, Iman M.; Fadhil, Hula Y.; Alhamdani, Faisal G.

doi:10.5958/0974-360x.2020.00459.x

Cited by 4 publications

(2 citation statements)

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“…In addition, patients with high HCoV viral load were more likely to be co-infected with other infectious diseases, such as Tuberculosis, since co-infections are mostly found among patients with high viral load in comparison to those having a low viral load [10]. Consequently, co-infection could increase the severity of respiratory tract illness [11]. The results obtained from the current study showed that Ct value and age had significant (p >0.001) inverse association, as shown in Figure 2, especially in people <60 years, as all cases in this group had Ct value lower than 30, which agrees with many studies that implied age as a possible risk factor for COVID-19 infection [6].…”

Section: -Age Gender and Cycle Threshold Value (Ct)mentioning

confidence: 99%

Estimation of Hematological Parameters of Disease Severity in Iraqi Patients with COVID-19

Mahmood

Fadhil

Ad’hiah

2021

eijs

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Coronavirus disease 2019 (COVID-19) is a systemic disease with a substantial impact on the hematopoietic system and hemostasis. Neutrophilia is an early indicator of SARS-CoV-2 infection, while lymphopenia acts as a biomarker of the severity of infection, and the neutrophil-to-lymphocyte ratio (NLR) is the main indicator of cytokine storms. Thus, this study aimed to provide local data about hematological parameters among COVID-19 patients and estimate their correlation with viral load and other factors in severe cases. A total of 99 nasopharyngeal swabs and whole blood specimens were collected from individuals suspected with COVID-19 between October and December 2020. Samples were tested by real time reverse transcriptase polymerase chain reaction (rRT-PCR) assay, COVID-19 IgG and IgM antibody tests, beside hematological analysis. The results showed a significant increase in neutrophils count and NLR, correlated with the severity of the disease Patients at older ages who are suffering from some comorbidity like hypertension and diabetes are at elevated risk to develop more severe disease outcome. The findings demonstrated a higher neutrophils count and higher death ratio in older ages. Also, the results suggest that NLR could be employed as a useful biomarker and potential prognostic tool supporting the importance of laboratory records in assessing case severity and disease progression.

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Section: -Age Gender and Cycle Threshold Value (Ct)mentioning

confidence: 99%

Estimation of Hematological Parameters of Disease Severity in Iraqi Patients with COVID-19

Mahmood

Fadhil

Ad’hiah

2021

eijs

Self Cite

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“…However, prompt appropriate knowledge should be kept in view to avoid susceptibility to HCV infection through factors: drug addiction, minor and major surgeries, barbers' shops and hemodialysis, etc. The major causes of viral spread are needle sharing, open wounds, and razor or nail cutter sharing (26,27) .…”

Section: Prevalence Of Subclinical Igm-hcv Positivitymentioning

confidence: 99%

Prevalence of Subclinical Cases of HBsAg and IgM-HCV Positivity among Healthy Iraqi Individuals

2024

The Egyptian Journal of Hospital Medicine

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Background: A significant proportion of the world's population suffers from chronic viral hepatitis B (HBV) and hepatitis C (HCV) infection, and both HBV and HCV are leading causes of chronic liver disease (CLD), leading to significant morbidity and mortality.Objective: This study aimed to understand the prevalence of subclinical cases of HBV-surface antigen (HBsAg) and IgM-HCV positivity among healthy individuals for early intervention and treatment through an enzyme-linked immune-sorbent assay test. Materials and methods: A total of 250 blood samples were collected from healthy individuals (HI) along with surgeries, before marriage, and blood donors. Results: showed that a reaction for HBsAg and IgM-HCV positivity was 16.4% (41/250) and 13.6% (34/250) of healthy individuals respectively as a sub-clinical case, and there was significant disappearance of the viral infection among HI. A higher frequency of viral occurrence was reported in ages between 21 to 40 years old. There was a significant increase in males 53.3% and 64.7% than in females 46.7% and 35.3% regarding HBsAg and IgM-HCV respectively. Furthermore, this study showed that there was significant HCV infection appearance in males than in females in contrast with HBsAg was not significant. There was a higher significant increase of the viral infection obtained among married (p < 0.001) females and males. This finding pointed out the reveal of viral infection mostly in individuals aged more than 30 years old accompanied by an increase with sexual contact. Conclusion: Screening protocols being mandatory in government agencies for at-risk populations can help identify subclinical cases and prevent further transmission.

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SARS-COV-2 (COVID-19) and role of real time Reverse Transcription Polymerase Chain Reaction (RT-PCR) in its diagnosis

Pinki¹

2021

RJPT

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The present article reviews the diagnosis of Novel Corona Virus Disease (COVID-19) by using Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR). COVID-19 is a contagious disease caused by severe acute respiratory syndrome coronavirus2 (SARS-CoV-2), which affects upper and lower respiratory tract. In present scenario COVID-19 is considered as global public health emergency by World Health Organization (WHO) so, the detection and prevention of this disease is vital to control this emergency. RT-PCR is a highly sensitive laboratory method for the detection of SARS-CoV-2. It is based on rapid diagnosis of SARS-CoV-2 by qualitative detection of its nucleic acid sequence. This test locates the primer and probe sets in different regions in the genome of SARS-CoV-2.

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Human Coronavirus Species and their correlation as co-infection detected by Fast Real-Time RT-PCR

Cited by 4 publications

References 0 publications

Estimation of Hematological Parameters of Disease Severity in Iraqi Patients with COVID-19

Estimation of Hematological Parameters of Disease Severity in Iraqi Patients with COVID-19

Prevalence of Subclinical Cases of HBsAg and IgM-HCV Positivity among Healthy Iraqi Individuals

SARS-COV-2 (COVID-19) and role of real time Reverse Transcription Polymerase Chain Reaction (RT-PCR) in its diagnosis

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