Human cytomegalovirus genetic variability in strains isolated from Japanese children during 1983-2003

Tanaka, Kaori; Numazaki, Kei; Tsutsumi, Hiroyuki

doi:10.1002/jmv.20366

Cited by 25 publications

(17 citation statements)

References 30 publications

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“…Multiple strains of virus circulate both within the population and within an individual, and evidence exists for inter-strain recombination [18][19]. There are no data on the genetic variants of HCMV in IBD.…”

Section: Discussionmentioning

confidence: 99%

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Criscuoli

Rizzuto

Gallo

et al. 2015

Journal of Clinical Virology

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a b s t r a c tBackground: Human Cytomegalovirus (HCMV) infection has been reported to be a cause of refractory ulcerative colitis (UC). Toxic megacolon (TM) is a rare but severe complication of an acute attack of UC. Objectives: Aim of this study is to evaluate in a case-control study the association between HCMV and TM. Study design: All patients who were admitted at Medicine Department of V. Cervello Hospital in Palermo (tertiary referral center) for a severe UC flare-up complicated by the onset of TM (diameter of the transverse colon > 6 cm) between January 1990 and November 2011 were identified through the electronic database. A total of 24 consecutive patients (16 male/8 female) with TM were identified. Each case of TM were individually matched by sex, age, extent of the underlying disease to 24 severe UC controls who did not develop TM. A further non matched control population of 48 severe UC was included.Haematoxilin and eosin stain, immunohistochemical procedure and nested polymerase chain reaction were performed to detect HCMV genes and proteins on rectal biopsies or surgical specimens. Pp65 antigenemia was performed in order to diagnose any possible systemic infection. HCMV frequency was compared between patients with and without TM during follow-up, using Fisher's Exact test. Results and conclusions: HCMV was detected in histological specimens of 11 patients (46%) with TM compared to 2 (9%) severe UC matched controls (P = 0.0078) and 7 (14%) unmatched controls (p = 0,003). In severe colitis the presence of HCMV is more frequently associated with TM.

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Section: Discussionmentioning

confidence: 99%

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Criscuoli

Rizzuto

Gallo

et al. 2015

Journal of Clinical Virology

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“…Bale et al [7] and Barbi et al [8] showed the dominance of gB1 genotype in the research of gB genotype distribution in cases of congenital HCMV infection. Tanaka et al [9] reported gB1 was the most common genotype in congenital HCMV infection in Japanese children although other genotypes also were found in those patients. These results differ significantly from results obtained for HIV-infected patients and bone marrow transplant recipients.…”

Section: Pcr Amplification Of Gb Gene and Restriction Analysismentioning

confidence: 99%

Human Cytomegalovirus Glycoprotein B Genotype Correlates with Different Symptoms of Infected Infants

Wang

2007

Intervirology

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Objectives: Human cytomegalovirus (HCMV) is the pathogen of cytomegalic inclusion disease of infants. HCMV strains can be classified into four genotypes of glycoprotein B (gB). There are limited data concerning links between clinical symptoms and HCMV genotypes. The aims of the present study were to determine the genotype of HCMV isolates from pediatric patients who have different symptoms on the assumption that the gB genotype may influence the outcomes of congenital and prenatal HCMV infection. Methods: The gB types of HCMV were determined in urine specimens from 208 infected infants using nested polymerase chain reaction and restriction fragment length polymorphism. Results: These data showed the dominance of the gB1 genotype in HCMV-infected infants. The distributions of HCMV gB genotypes in jaundice, malformation, and pneumonia patients are different. Conclusion: There are some relationships between the gB genotypes and the different symptoms of HCMV infection. The four gB genotypes of HCMV may have different clinical outcomes in infected infants.

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“…CMV-infected individuals may show characteristic symptoms at different times, as well as may show coinfection at the same time (19), which indicates reactivation of the latent infection. Hommes et al reported that colonic CMV disease in UC patients occurred only in seropositive patients, showing it to be a reactivation rather than a pri-mary infection (20).…”

Section: Discussionmentioning

confidence: 99%

The Absence of Large Ulcer Predicts Latent Cytomegalovirus Infection in Ulcerative Colitis with Positive Mucosal Viral Assay

et al. 2010

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Objective Although many studies have shown that cytomegalovirus (CMV) infection is an exacerbating factor in patients with ulcerative colitis (UC), there is no valid method to distinguish CMV infection requiring therapy from that disappearing without therapy. The aim of this study was to describe whether or not the endoscopic feature of a large ulcer predicts the necessity of antiviral therapy against CMV infection in active UC patients with positive mucosal viral assay. Methods Active UC patients in whom CMV infection was detected by mucosal polymerase chain reaction (PCR) assay were enrolled in this prospective observational clinical study. Patients with a large ulcer (ulcerated group) were treated with antiviral and UC therapy. Patients without a large ulcer (non-ulcerated group) were treated with only UC therapy. We prospectively evaluated the clinical and endoscopic findings in all of the patients 2 months after starting this protocol, and observed their outcomes during one year. Results In the ulcerated group (n=10), 3 patients still had active disease at 2 months and underwent colectomy. Although the other 7 patients achieved remission at 2 months, 4 of the 7 patients had a flare-up, and the remaining 3 patients maintained remission. All of the patients in the non-ulcerated group (n=10) attained remission without antiviral therapy at 2 months, and maintained remission. Conclusion In active UC patients with positive CMV DNA by mucosal PCR assay, the absence of a large ulcer suggests latent CMV infection, and requires no antiviral therapy.

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Human cytomegalovirus genetic variability in strains isolated from Japanese children during 1983-2003

Cited by 25 publications

References 30 publications

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Human Cytomegalovirus Glycoprotein B Genotype Correlates with Different Symptoms of Infected Infants

The Absence of Large Ulcer Predicts Latent Cytomegalovirus Infection in Ulcerative Colitis with Positive Mucosal Viral Assay

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