2013
DOI: 10.1128/jvi.01705-13
|View full text |Cite|
|
Sign up to set email alerts
|

Human Cytosolic Extracts Stabilize the HIV-1 Core

Abstract: bThe stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects on HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure the stability of in vitro-assembled HIV-1 CA-NC complexes. The assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core. Interestingly, stabilization of in vitro-assembled HIV-1 CA-NC complexes is not due solely to macromolecular … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

10
85
0

Year Published

2014
2014
2024
2024

Publication Types

Select...
5
1

Relationship

2
4

Authors

Journals

citations
Cited by 53 publications
(95 citation statements)
references
References 48 publications
10
85
0
Order By: Relevance
“…Next, we tested the ability of BI-2 to stabilize in vitro assembled HIV-1 CA-NC complexes using our previously published assay [2]. As we have previously shown, BI-2 as well as PF74 stabilize HIV-1 CA-NC complexes ( Figure 2C) [2]. These results showed that BI-2, like PF74, stabilizes in vitro assembled HIV-1 CA-NC complexes, which is in agreement with previous reports [1,2].…”
Section: Bi-2 Destabilizes the Hiv-1 Core During Infectionsupporting
confidence: 79%
See 4 more Smart Citations
“…Next, we tested the ability of BI-2 to stabilize in vitro assembled HIV-1 CA-NC complexes using our previously published assay [2]. As we have previously shown, BI-2 as well as PF74 stabilize HIV-1 CA-NC complexes ( Figure 2C) [2]. These results showed that BI-2, like PF74, stabilizes in vitro assembled HIV-1 CA-NC complexes, which is in agreement with previous reports [1,2].…”
Section: Bi-2 Destabilizes the Hiv-1 Core During Infectionsupporting
confidence: 79%
“…As a control, we showed that TRIM5α rh destabilizes the HIV-1 core ( Figure 2B), as previously shown [12,13]. Next, we tested the ability of BI-2 to stabilize in vitro assembled HIV-1 CA-NC complexes using our previously published assay [2]. As we have previously shown, BI-2 as well as PF74 stabilize HIV-1 CA-NC complexes ( Figure 2C) [2].…”
Section: Bi-2 Destabilizes the Hiv-1 Core During Infectionmentioning
confidence: 74%
See 3 more Smart Citations