Human Dermal Fibroblast Subpopulations Are Conserved across Single-Cell RNA Sequencing Studies

Ascensión, Alex M.; Fuertes-Álvarez, Sandra; Ibáñez-Solé, Olga; Izeta, Ander; Araúzo-Bravo, Marcos J.

doi:10.1016/j.jid.2020.11.028

Cited by 89 publications

(108 citation statements)

References 37 publications

Supporting

Mentioning

105

Contrasting

Order By: Relevance

“…[36][37][38][39][40][41][42] Marker combinations to identify skin fibroblast lineages and sub-populations have been established for mouse (eg, Eng1, Sca1, Blimp1, Dlk1, Lrig1, Blimp1/Prdm1, CD26, and Lef1), [43][44][45] and human skin tissue (eg, RGS5, CD26, CD36, and CD39). 38 Metaanalyses of multiple scRNAseq datasets for the same organ like skin and lung 37,46 or across different organs 41 will help to identify universal fibroblast denominators. So far, these studies, at high technical level, confirmed observations from the last century that fibroblasts are heterogenous and diverse.…”

Section: What Is a Fibroblast?mentioning

confidence: 99%

“…The question of fibroblast identity is further complicated by the fact that fibroblast (and MSC) populations can derive from different lineages and express organ‐specific molecular markers, despite playing similar roles. Even within one organ, different fibroblast (and MSC) populations exist, either by arising from different embryonic progenitors or by adapting to the positional information provided by their respective niche, which is particularly well studied in the skin 36‐42 . Marker combinations to identify skin fibroblast lineages and sub‐populations have been established for mouse (eg, Eng1, Sca1, Blimp1, Dlk1, Lrig1, Blimp1/Prdm1, CD26, and Lef1), 43‐45 and human skin tissue (eg, RGS5, CD26, CD36, and CD39) 38 .…”

Section: Fibroblasts and Their Role In Tissue Repair And Fibrosismentioning

confidence: 99%

See 1 more Smart Citation

The inflammatory speech of fibroblasts

Schuster

Rockel

Kapoor

et al. 2021

Immunological Reviews

110

View full text Add to dashboard Cite

Successful tissue repair after injury requires the complex interplay of multiple cell types in different activation states in the context of an extracellular matrix (ECM) with changing compositional and mechanical properties. Normal repair occurs in a highly regulated sequence of overlapping phases comprising hemostasis, inflammation, proliferation, remodeling, and maturation. 1,2 With breached or leaky vasculature, platelets exit the bloodstream and create a provisional ECM that serves as initial scaffold for immune cells and growth factors.Mast cells are early responders, followed by neutrophils that fight invading microorganisms and create a local environment of cytokines and hypoxia that stimulate the recruitment and/or activation of macrophages. While clearing wound tissue from debris and dead cells,

show abstract

Section: What Is a Fibroblast?mentioning

confidence: 99%

Section: Fibroblasts and Their Role In Tissue Repair And Fibrosismentioning

confidence: 99%

The inflammatory speech of fibroblasts

Schuster

Rockel

Kapoor

et al. 2021

Immunological Reviews

110

View full text Add to dashboard Cite

show abstract

“…They synthesize and remodel the extracellular matrix and maintain the structural integrity of tissues [15]. Individual fibroblasts exhibit differences in gene expression that allowed the description of sub-populations, accounting for the fibrogenic, fibrolytic, and inflammatory activity of these cells [16]- [18]. Following tissue damage, fibroblasts experience phenotypic interconversion by adopting matrix secreting and inflammatory states [19], [20].…”

Section: Introductionmentioning

confidence: 99%

Sphingolipid Control of Fibroblast Heterogeneity Revealed by Single-Cell Lipidomics

Capolupo

Khven

Mazzeo

et al. 2021

Preprint

View full text Add to dashboard Cite

Human cells produce thousands of lipids that impact a wide range of biological processes in ways we are only starting to characterize. The cellular composition in lipids changes during differentiation events and also varies across individual cells of the same type. Yet, the precise differences in lipid composition that directly affect cell phenotypes remain unknown. Here we have measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling high-resolution mass spectrometry imaging to single-cell transcriptomics. We found that the cell-to-cell variation of specific lipid metabolic pathways contributes to the establishment of cell states involved in wound repair and in skin cancer growth. Sphingolipid composition defined fibroblast subpopulations while sphingolipid metabolic rewiring drove cell state transitions. These data uncover a role for cell-to-cell lipid heterogeneity in the determination of cell states and reveal a new regulatory component to the homeostasis and self-organization of multicellular systems.

show abstract

“…At the same time, there are also some methodological and technical difficulties at this stage: (1) Most Fb subtype surface markers are co-expressed, thus it is challenging to manipulate and to perform research on a specific group of Fbs (Ascensión et al, 2020). (2) It has been suggested that the phenotype of Fb subtypes requires a specific in vivo microenvironment (Korosec et al, 2019),which is, however, hard to maintain after in vitro culture, indicating that functional profiling directly after cell isolation might provide more reliable information than in vitro experiments.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

“…With the help of omics technology, fibroblast subpopulations were studied in detail and were found to be highly heterogeneous in their compositions. Previously characterized papillary and reticular Fb were demonstrated to contain a complex population of cells that do not share a specific marker (Ascensión et al, 2020;Vorstandlechner et al, 2020). At present, several studies have been performed to uncover the Fb heterogeneity in normal human skin tissues.…”

Section: Introductionmentioning

confidence: 99%

Targeting Dermal Fibroblast Subtypes in Antifibrotic Therapy: Surface Marker as a Cellular Identity or a Functional Entity?

et al. 2021

View full text Add to dashboard Cite

Fibroblasts are the chief effector cells in fibrotic diseases and have been discovered to be highly heterogeneous. Recently, fibroblast heterogeneity in human skin has been studied extensively and several surface markers for dermal fibroblast subtypes have been identified, holding promise for future antifibrotic therapies. However, it has yet to be confirmed whether surface markers should be looked upon as merely lineage landmarks or as functional entities of fibroblast subtypes, which may further complicate the interpretation of cellular function of these fibroblast subtypes. This review aims to provide an update on current evidence on fibroblast surface markers in fibrotic disorders of skin as well as of other organ systems. Specifically, studies where surface markers were treated as lineage markers and manipulated as functional membrane proteins are both evaluated in parallel, hoping to reveal the underlying mechanism behind the pathogenesis of tissue fibrosis contributed by various fibroblast subtypes from multiple angles, shedding lights on future translational researches.

show abstract

Human Dermal Fibroblast Subpopulations Are Conserved across Single-Cell RNA Sequencing Studies

Cited by 89 publications

References 37 publications

The inflammatory speech of fibroblasts

The inflammatory speech of fibroblasts

Sphingolipid Control of Fibroblast Heterogeneity Revealed by Single-Cell Lipidomics

Targeting Dermal Fibroblast Subtypes in Antifibrotic Therapy: Surface Marker as a Cellular Identity or a Functional Entity?

Contact Info

Product

Resources

About