Human Enamel as a Substrate for in Vitro Acid Dissolution Studies: Influence of Tooth Surface and Morphology

Tucker, K. G.; Adams, Monica; Shaw, Linda; Smith, Anthony J.

doi:10.1159/000016443

Cited by 23 publications

(14 citation statements)

References 35 publications

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“…Thus it can be concluded that only sound enamel (not previously damaged) had been used. Regarding mineral loss and lesion depth, a comparable trend (tables 1, 2) could be observed in all panelists, suggesting that the origin of samples did not have any considerable influence [Tucker et al, 1998]. However, the extent of incipient (early) caries developed in this in situ study varied extremely.…”

Section: Discussionsupporting

confidence: 63%

Microradiographic and Microscopic Studies on in situ Induced Initial Caries in Irradiated and Nonirradiated Dental Enamel

1999

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The objective of this study was to evaluate the onset of initial demineralization in irradiated and nonirradiated human enamel. Enamel specimens were prepared from the lingual and buccal surfaces of 48 freshly extracted, caries–free third molars. Either the lingual or the buccal enamel specimen of each tooth was irradiated with 60 Gy. The remaining enamel sample was not irradiated. Two irradiated and two nonirradiated enamel specimens were inserted into both buccal aspects of each 12 intraoral mandibular appliances. The appliances were worn by 12 persons for 6 weeks throughout day and night. One side was brushed daily with a fluoride–free toothpaste. On the other side plaque was allowed to grow. Individual oral hygiene techniques were performed without any fluorides. During meals, the appliance was stored in 10% sucrose solution. The enamel specimens were cut perpendicular to the enamel surface. Subsequently, the slabs were ground to a thickness of 90 μm, and studied by means of TMR and microscopic techniques. Evaluated data did not show any differences between irradiated and nonirradiated enamel lesions (ANOVA). The onset of caries in irradiated enamel can be hampered by regularly performed oral hygiene techniques.

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Section: Discussionsupporting

confidence: 63%

Microradiographic and Microscopic Studies on in situ Induced Initial Caries in Irradiated and Nonirradiated Dental Enamel

1999

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“…Furthermore, it was noted that some of the test subjects did not show a continuous decrease in enamel wear within the 60-min remineralization period. This finding might be explained by discrepancies between specimens prepared from various teeth, which account for the different susceptibility of specimens to erosion and abrasion [Davis and Winter, 1980;Attin et al, 1997;Tucker et al, 1998]. Moreover, in the present study, salivary pellicles were allowed to form on the surfaces of the specimens in the intervals (of at least 4 h) between the erosion/brushing steps.…”

Section: Resultsmentioning

confidence: 81%

In situ Evaluation of Different Remineralization Periods to Decrease Brushing Abrasion of Demineralized Enamel

et al. 2001

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The aim of the present in situ study was to evaluate the effect of different periods of intraoral remineralization to decrease the susceptibility of previously demineralized enamel against toothbrushing abrasion. Six human enamel specimens (A–F) were recessed in the buccal aspects of each of eight intraoral appliances which were worn for 21 days by 8 panelists. Demineralization of the samples was performed twice a day extraorally in the acidic beverage Sprite Light for 90 s. Subsequently, the enamel specimens were brushed at different times. Specimen A was brushed immediately after the demineralization. The remaining samples B–E were brushed after the intraoral appliances had been worn for various periods of remineralization: specimen B, 10 min; C, 20 min; D, 30 min and E, 60 min, respectively. Specimen F was only demineralized and remineralized, but not brushed. After 21 days, enamel wear was measured with a laser profilometer. The following values (mean ± standard deviation) were obtained: specimen A, 6.78±2.71 µm; B, 5.47±3.39 µm; C, 6.06±3.18 µm; D, 5.43±2.58 µm; E 4.78±2.57 µm, and F 0.66±1.11 µm. Analysis of variance revealed a significant influence of remineralization period on abrasive wear. However, even after a remineralization period of 60 min the wear was significantly increased as compared to the demineralized, but not brushed control. It is concluded that (1) abrasion resistance of softened enamel increases with remineralization period and (2) at least 60 min should elapse before toothbrushing after an erosive attack.

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“…Mineral loss from the tooth surface including calcium release was found to increase in association with increased acid challenge time 12,13,30,32) . However, Ca decreased after 6 cycles in both groups.…”

Section: Discussionmentioning

confidence: 96%

“…Many laboratory studies on dental erosion have focused on the chemical aspects of the erosive potential of acidic foods and beverages. In those erosion related studies, polished enamel samples were used to reduce the possible influence of superficial variations such as high fluoride content that may influence remineralization and demineralization cycles, and different ion concentrations 13,14) . Carvalho and Lussi published results on the susceptibility of dental enamel to erosive dissolution in relation to different types and surfaces of teeth, as well as the enamel depth by using polished enamel samples.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of the susceptibility of cut and uncut enamel to erosive demineralization

Lin

Kitasako

Nakashima

et al. 2017

Dental Materials Journal

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This study aimed to evaluate the susceptibility of cut and uncut enamel surfaces to an erosive challenge and to examine the resultant characteristics/morphological changes. Ten extracted human incisors were used for preparation of enamel samples, and samples were immersed in citric acid. After 3 (total 3 min) and 6 cycles (total 6 min) of erosive challenges, surface loss (SL) and morphological changes were measured using scanning microscopy and FIB-TEM. Ca release (CA) and surface hardness (SH) were measured using a calcium-sensitive electrode and hardness tester respectively. Mean values of all measurements were statistically analyzed by using a t-test. Uncut enamel samples had significantly lower SL and greater SH than cut enamel (p<0.01). Cut enamel samples after 3 cycles showed higher CA compared with those from uncut enamel samples (p<0.05). Cut enamel was shown to be more susceptible to acidic dissolution and deeper acid penetration than uncut enamel after erosive demineralization.

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Human Enamel as a Substrate for in Vitro Acid Dissolution Studies: Influence of Tooth Surface and Morphology

Cited by 23 publications

References 35 publications

Microradiographic and Microscopic Studies on in situ Induced Initial Caries in Irradiated and Nonirradiated Dental Enamel

Microradiographic and Microscopic Studies on in situ Induced Initial Caries in Irradiated and Nonirradiated Dental Enamel

In situ Evaluation of Different Remineralization Periods to Decrease Brushing Abrasion of Demineralized Enamel

A comparative study of the susceptibility of cut and uncut enamel to erosive demineralization

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