Human erythrocyte and plasma amino acid concentrations during exercise

MacLaren, Donald; Nevill, Alan M.; Thake, Doug; Campbell, Iain; Cheetham, E.; Keegan, Mary; Lane, C.; Roberts, N. B.

doi:10.1097/00005768-200007000-00010

Cited by 10 publications

(19 citation statements)

References 20 publications

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“…Thus, significant equivalent increases of 10% in plasma tHcy have been observed 30 min after running on a treadmill (39) or 19% in marathon runners 24 h after finishing the race (22). Resting plasma amino acid concentrations were within the range of previously reported values (17). These resting concentrations varied significantly between sports and differences may be due to minor variations in protein content of the diet before the overnight fast and to different body mass index or physical training.…”

Section: Discussionsupporting

confidence: 66%

Plasma Vitamins, Amino Acids, and Renal Function in Postexercise Hyperhomocysteinemia

Venta

Cruz

Valcárcel

et al. 2009

Medicine &Amp; Science in Sports &Amp; Exercise

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Section: Discussionsupporting

confidence: 66%

Plasma Vitamins, Amino Acids, and Renal Function in Postexercise Hyperhomocysteinemia

Venta

Cruz

Valcárcel

et al. 2009

Medicine &Amp; Science in Sports &Amp; Exercise

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“…It was shown that erythrocytes and plasma have very different profile characteristics where at least eight amino acids were measured at significantly higher levels in the erythrocytes than the corresponding plasma and two amino acids (glutamine and arginine) were found to be lower (p < 0.01) (Agli et al 1998). Aspartic acid showed contrasting levels in erythrocytes and plasma analysed from resting blood samples, with 451-484 µM in the erythrocytes compared with 14.5-15.1 µM in the corresponding plasma (MacLaren et al 2000).…”

Section: Introductionmentioning

confidence: 88%

“…Amino acid levels in human plasma and erythrocytes were found to increase significantly in the post-absorptive state following an oral loading of amino acids (Agli et al 1998). A separate study in a human exercise challenge demonstrated that erythrocytes increased their carrying capacity of amino acids during exercise with no corresponding change in the plasma levels (MacLaren et al 2000). These investigations have made important and relevant contributions providing evidence that the amino acid concentrations in erythrocytes are acutely sensitive to lifestyle interventions and alter post-absorption or in response to exercise.…”

Section: Introductionmentioning

confidence: 94%

Evidence that human and equine erythrocytes could have significant roles in the transport and delivery of amino acids to organs and tissues

et al. 2020

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Erythrocytes have a well-defined role in the gaseous exchange of oxygen and carbon dioxide in the mammalian body. The erythrocytes can contain more than half of the free amino acids present in whole blood. Based on measures showing that venous erythrocyte levels of amino acids are much less than arterial erythrocyte levels, it has previously been proposed that erythrocytes also play a role in the delivery of amino acids to tissues in the body. This role has been dismissed because it has been assumed that to act as an amino acid transport vehicle, the erythrocytes should release their entire amino acid content in the capillary beds at the target tissues with kinetic studies showing that this would take too long to achieve. This investigation set out to investigate whether the equine erythrocytes could rapidly take up and release smaller packages of amino acids when exposed to high or low external concentrations of amino acids, because it seemed very unlikely that cells would be able to release all of their amino acids without serious impacts on osmotic balance. Freshly prepared erythrocytes were placed in alternating solutions of high and low amino acid concentrations in PBS to assess the capacities of these cells to rapidly take up and release amino acids depending on the nature of the external environment. It was found that amino acids were rapidly taken up and released in small quantities in each cycle representing 15% of their total load in equine erythrocytes and 16% in human erythrocytes. The capacity for rapid uptake/release of amino acids by equine and human erythrocytes provided evidence to support the theory that mammalian erythrocytes have a significant role in transport of amino acids from the liver to tissues, muscles and organs.

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“…[1][2][3][4][5][6] For over thirty years most amino acid analyses have been performed by ion-exchange chromatography with post-column derivatization with ninhydrin. [7] Alternatively, amino acids can be analyzed by precolumn fluorescent derivatization and then separated by reverse-phase high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid quantitative high-performance liquid chromatographic analysis of plasma amino acids

Frank

Powers

2007

Journal of Chromatography B

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A simple and rapid high performance liquid chromatographic method for the determination of plasma amino acids was developed. The method uses minimal sample volume and automated online precolumn derivitization of amino acids with o-phthalaldehyde and fluorescent detection. Amino acids are separated by a simplified gradient without column heating. The assay is linear from 5 to 1000μmol/L for all amino acids. Recovery of amino acids was between 91 and 108%, intra-assay coefficient of variation (CV) was 1-7%, and inter-assay CV was 2-12%. The simple sample preparation and minimal sample volume make the method useful for the quantitation of amino acids in both patient and experimental animal samples.

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Human erythrocyte and plasma amino acid concentrations during exercise

Cited by 10 publications

References 20 publications

Plasma Vitamins, Amino Acids, and Renal Function in Postexercise Hyperhomocysteinemia

Plasma Vitamins, Amino Acids, and Renal Function in Postexercise Hyperhomocysteinemia

Evidence that human and equine erythrocytes could have significant roles in the transport and delivery of amino acids to organs and tissues

Simple and rapid quantitative high-performance liquid chromatographic analysis of plasma amino acids

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