Human erythrocytes are affected in vitro by flavonoids of Aristotelia chilensis (Maqui) leaves

Suwalsky, Mario; Vargas, Pedro; Avello, Marcia; Villena, Fernando Pardo-Manuel de; Sotomayor, Carlos P.

doi:10.1016/j.ijpharm.2008.07.005

Cited by 72 publications

(33 citation statements)

References 33 publications

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“…A previous study reported that Balbisia peduncularis extract caused changes in the normal erythrocyte morphology observed under an SEM (Suwalsky et al, 2008). The same study also observed the formation of echinocytes, stomatocytes, and spherocytes (Suwalsky et al, 2008).…”

Section: Discussionmentioning

confidence: 84%

“…Studies had shown that RBCs respond to various treatments by various agents by altering their morphological features (Przybylska et al, 1998). A previous study reported that Balbisia peduncularis extract caused changes in the normal erythrocyte morphology observed under an SEM (Suwalsky et al, 2008). The same study also observed the formation of echinocytes, stomatocytes, and spherocytes (Suwalsky et al, 2008).…”

Section: Discussionmentioning

confidence: 93%

“…The bilayer couple theory of membrane explains the interaction of amphiphatic drug membrane with human RBCs (Sheetz and Singer, 1974). An in vitro study performed by Suwalsky et al(2008) showed that Aristotelia chilensis aqueous extracts were able to induce morphological changes of RBCs from normal discoid shape to echinocyte form. The changes explained the interaction of the extract with the membrane's outer phospholipids monolayer.…”

Section: Discussionmentioning

confidence: 99%

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Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Taib

Budin

Ain

et al. 2009

J. Zhejiang Univ. Sci. B

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Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Taib

Budin

Ain

et al. 2009

J. Zhejiang Univ. Sci. B

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“…The antioxidant activity of some flavonoids, including anthocyanin, is markedly higher than the activity of vitamin E, and its synthetic equivalent Trolox ® . They also effectively inhibit erythrocyte hemolysis induced by free radicals, protecting the erythrocyte membrane against oxidative destruction [5,10,[13][14][15][16][17][18]]. An exposed and very good site of attack by free radicals in an organism is the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Bonarska-Kujawa

Pruchnik

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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Anthocyanins are one of the main flavonoid groups. They are responsible for, e.g., the color of plants and have antioxidant features and a wide spectrum of medical activity. The subject of the study was the following compounds that belong to the anthocyanins and which can be found, e.g., in strawberries and chokeberries: callistephin chloride (pelargonidin-3-O-glucoside chloride) and ideain chloride (cyanidin-3-O-galactoside chloride). The aim of the study was to determine the compounds' antioxidant activity towards the erythrocyte membrane and changes incurred by the tested anthocyanins in the lipid phase of the erythrocyte membrane, in liposomes composed of erythrocyte lipids and in DPPC, DPPC/cholesterol and egg lecithin liposomes. In particular, we studied the effect of the two selected anthocyanins on red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC and DPPC/cholesterol liposomes. Fluorimetry with the Laurdan and Prodan probes indicated increased packing density in the hydrophilic phase of the membrane in the presence of anthocyanins. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The compounds slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The study has shown that both anthocyanins are incorporated into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The investigation proved that the compounds penetrate only the outer part of the external lipid layer of liposomes composed of erythrocyte lipids, DPPC, DPPC/cholesterol and egg lecithin lipids, changing its packing order. Fluorimetry studies with DPH-PA proved that the tested anthocyanins are very effective antioxidants. The antioxidant activity of the compounds was comparable with the activity of Trolox ® .

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“…[17][18][19] The off-line DPPH radical scavenging activity of the three target compounds, luteolin-3'-O-β-D-glucopyranoside (1), luteolin-7-O-β-D-glucopyranoside (2) and luteolin-4'-O-β-Dglucopyranoside (3), was evaluated. The results showed that compounds 1-3 had potent free radical scavenging capacities with IC 50 values of 13.12, 9.58 and 13.65 μmol L -1 , respectively.…”

Section: Antioxidant Activity Of Target Compoundsmentioning

confidence: 99%

Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun

Jiang¹,

Shi²,

Zhang³

et al. 2010

J. Braz. Chem. Soc.

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Em nossas pesquisas anteriores encontramos que o extrato bruto de Neo-T. siphonanthun apresentou atividade eficaz para captura de radicais DPPH (1,1-difenil-2-picrilidazida). Neste estudo, um método de triagem rápida online, através de um sistema de cromatografia líquida de alta eficiência com detector por captura de radicais, acoplada a detector por arranjo de diodos e espectrometria de massas com ionização por eletrospray (HPLC-RSD-DAD-ESI/MS) foi desenvolvido para a procura de antioxidantes individuais a partir da fração mais ativa. Três derivados isoméricos foram detectados. Os compostos-alvo ativos foram isolados por cromatografia em contracorrente de alta velocidade (CCC-AV) com pureza superior a 99%, e foram identificados como luteolina-3'-O-β-D-glicopiranosídeo (1), luteolina-7-O-β-D-glicopiranosídeo (2) e luteolina-4'-O-β-D-glicopiranosídeo (3) por análise de seus espectros de ressonância magnética nuclear (RMN). A atividade antioxidante dos três compostos foi avaliada pelo ensaio DPPH off-line, e todos eles mostraram atividade potente.Our previous research found that the crude extract of Neo-T. siphonanthun exhibited an effective DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging activity. In this study an online rapid screening method, high-performance liquid chromatography-radical scavenging detection-diode array detector-electrospray ionization mass spectrometry (HPLC-RSD-DAD-ESI/MS) system, was developed for screening individual antioxidants from the most active fraction. Accordingly, three isomeric derivatives were detected. The target active compounds were isolated by highspeed counter-current chromatography (HSCCC) with the purity over 99%, and were identified as luteolin-3'-O-β-D-glucopyranoside (1), luteolin-7-O-β-D-glucopyranoside (2) and luteolin-4'-O-β-D-glucopyranoside (3) by analysis of its off-line nuclear magnetic resonance (NMR) spectra. Antioxidant activity of three compounds was assessed by off-line DPPH assay, and all of them showed potent activity.

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Human erythrocytes are affected in vitro by flavonoids of Aristotelia chilensis (Maqui) leaves

Cited by 72 publications

References 33 publications

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun

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