Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.

Peltz, Gary; Grundy, Howard; Lebo, Roger V.; Yssel, Hans; Barsh, Gregory S.; Moore, Kevin W.

doi:10.1073/pnas.86.3.1013

Cited by 113 publications

(34 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of overlapping expressed sequence tags (AA693721, AA746047, and N36002) corresponding to the 3Ј region of a novel gene of unknown function and sequences homologous to genes encoding the low affinity IgG Fc receptors Fc␥RII (CD32) and Fc␥RIII (CD16) were identified. Three Fc␥RII genes (FCGR2A, -B, and -C) and two Fc␥RIII genes (FCGR3A and -B) have been described and physically mapped to a region of Ϸ200 kb in 1q22 (21,24,27,30). Accordingly, FISH with PAC986b4 on normal lympho- cyte prometaphase chromosomes showed signals consistently localizing to proximal 1q22 (Fig.…”

Section: Southern Blot Analysis and Cloning Of The T(1;22)(q21;q11) Bmentioning

confidence: 99%

The IgG Fc receptor, FcγRIIB, is a target for deregulation by chromosomal translocation in malignant lymphoma

Callanan

Baccon

Mossuz

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Rearrangement of chromosomal bands 1q21–23 is one of the most frequent chromosomal aberrations observed in hematological malignancy. The genes affected by these rearrangements remain poorly characterized. Typically, 1q21–23 rearrangements arise during tumor evolution and accompany disease-specific chromosomal rearrangements such as t(14;18) ( BCL2 ) and t(8;14) ( MYC ), where they are thus thought to play an important role in tumor progression. The pathogenetic basis of this 1q21–23-associated disease progression is currently unknown. In this setting, we surveyed our series of follicular lymphoma for evidence of recurring 1q21–23 breaks and identified three cases in which a t(14;18)(q32;q21) was accompanied by a novel balanced t(1;22)(q22;q11). Molecular cloning of the t(1;22) in a cell line (B593) derived from one of these cases and detailed fluorescent in situ hybridization mapping in the two remaining cases identified the FCGR2B gene, which encodes the immunoreceptor tyrosine-based inhibition motif-bearing IgG Fc receptor, FcγRIIB, as the target gene of the t(1;22)(q22;q11). We demonstrate deregulation of FCGR2B leading to hyperexpression of FcγRIIb2 as the principal consequence of the t(1;22). This is evidence that IgG Fc receptors can be targets for deregulation through chromosomal translocation in lymphoma. It suggests that dysregulation of FCGR2B may play a role in tumor progression in follicular lymphoma.

show abstract

Section: Southern Blot Analysis and Cloning Of The T(1;22)(q21;q11) Bmentioning

confidence: 99%

The IgG Fc receptor, FcγRIIB, is a target for deregulation by chromosomal translocation in malignant lymphoma

Callanan

Baccon

Mossuz

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…ADCC utilizes the IgG Fc-gamma receptor (Fc␥R, FC␥RIIIa, or CD16a) expressed on natural killer (NK) cells but also in other immune cell types (e.g., macrophages and neutrophils) as a means of bringing these cells into contact with antibody-coated cells (62). The Fab portion of the antibody binds the antigen at the surface of the target cell, and the Fc portion binds Fc␥R on the effector cells.…”

Section: Discussionmentioning

confidence: 99%

Interaction with Cellular CD4 Exposes HIV-1 Envelope Epitopes Targeted by Antibody-Dependent Cell-Mediated Cytotoxicity

Veillette

Désormeaux

Medjahed

et al. 2014

J Virol

249

600

View full text Add to dashboard Cite

Anti-HIV-1 envelope glycoprotein (Env) antibodies without broadly neutralizing activity correlated with protection in the RV144 clinical trial, stimulating interest in other protective mechanisms involving antibodies, such as antibody-dependent cellmediated cytotoxicity (ADCC). Env epitopes targeted by many antibodies effective at mediating ADCC are poorly exposed on the unliganded Env trimer. Here we investigated the mechanism of exposure of ADCC epitopes on Env and showed that binding of Env and CD4 within the same HIV-1-infected cell effectively exposes these epitopes. Env capacity to transit to the CD4-bound conformation is required for ADCC epitope exposure. Importantly, cell surface CD4 downregulation by Nef and Vpu accessory proteins and Vpu-mediated BST-2 antagonism modulate exposure of ADCC-mediating epitopes and reduce the susceptibility of infected cells to this effector function in vitro. Significantly, Env conformational changes induced by cell surface CD4 are conserved among Env from HIV-1 and HIV-2/SIVmac lineages. Altogether, our observations describe a highly conserved mechanism required to expose ADCC epitopes that might help explain the evolutionary advantage of downregulation of cell surface CD4 by the HIV-1 Vpu and Nef proteins. IMPORTANCEHIV-1 envelope epitopes targeted by many antibodies effective at mediating antibody-dependent cell-mediated cytotoxicity (ADCC) are poorly exposed on the unliganded envelope trimer. Here we investigated the mechanism of exposure of these epitopes and found that envelope interaction with the HIV-1 CD4 receptor is required to expose some of these epitopes. Moreover, our results suggest that HIV-1 CD4 downregulation might help avoid the killing of HIV-1-infected cells by this immune mechanism.

show abstract

“…pH 7.4). The FcTRIII transcript was detected using the pGP5 clone for FcTRill [8]. The human catalase transcript was detected using the 0.8 kb insert from a pSP64 clone following Hindlll aml gcoRI digestion.…”

Section: Northern Hloltingmentioning

confidence: 99%

Decreased expression of FcγRIII mRNA in leukemic granulocytes

Monteiro¹,

Advani

Gothoskar³

et al. 1992

FEBS Letters

View full text Add to dashboard Cite

Morphologically mature granulocytes from patients with chronic myeloid leukemia show significant impairment in their ability to internalize aggregated IgG, a ligand that is rapidly phagocytosed by normal human granulocytes. With a view to understand the molecular basis of this defect, normal and leukemic granulocytes were examined for the steady-state levels ofmRNA for Fc?'RIIL a memb=are-associat~xl receptur that initially binds and traps the !gG-opsonized antigens. Northern blot analyses revealed that the level ofthe specific mRNA in CP,[L granulocytes was between 0.08 and 0.69 times that seen in the normal granulocytes. This could be one of the contributory factors for the observed endocytic defect in the leukemic granulocytes.

show abstract

Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.

Abstract: A cDNA clone encoding a human receptor for

Cited by 113 publications

References 46 publications

The IgG Fc receptor, FcγRIIB, is a target for deregulation by chromosomal translocation in malignant lymphoma

The IgG Fc receptor, FcγRIIB, is a target for deregulation by chromosomal translocation in malignant lymphoma

Interaction with Cellular CD4 Exposes HIV-1 Envelope Epitopes Targeted by Antibody-Dependent Cell-Mediated Cytotoxicity

Decreased expression of FcγRIII mRNA in leukemic granulocytes

Contact Info

Product

Resources

About