Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces

Abstract

Applying antibacterial coatings to dental implant materials seems reasonable but can have negative influences on desired cell adhesion and healing. In this study, zirconia abutment specimens interacting with gingival tissue were used. The aim was to compare the influence of machined or coated zirconia surfaces on the adhesion and proliferation of human gingival fibroblasts (HGF-1). Surface modifications were performed using atmospheric plasma coating with hydroxyapatite, zinc, and copper. Zirconia specimens were divided into four groups: hydroxyapatite, hydroxyapatite with zinc oxide (ZnO), hydroxyapatite with copper (Cu), and an untreated machined surface. After the characterization of the surface conditions, the morphology of adhered HGF-1 was determined by fluorescence staining and subjected to statistical evaluation. The visual analysis of cell morphology by SEM showed flat, polygonal, and largely adherent fibroblast cells in the untreated group, while round to partially flat cells were recorded in the groups with hydroxyapatite, hydroxyapatite + ZnO, and hydroxyapatite + Cu. The cell membranes in the hydroxyapatite + ZnO and hydroxyapatite + Cu groups appeared porous. The results show that HGF-1 adhere and proliferate well on machined zirconia, while plasma coating with hydroxyapatite or hydroxyapatite mixtures does not lead to increased adhesion or proliferation.

Titanium surfaces with biomimetic topography and copper incorporation to modulate behaviors of stem cells and oral bacteria

“…More interestingly, the appearance of petal-like nanostructures is similar to the cross-section of tooth enamel after acid etching, i.e., the most highly mineralized tissue in the human body ( Cassari et al, 2023 ; Wu et al, 2023 ). This result suggests that this nanometer topography plays a significant role in regulating the cells’ fate and micro/nanostructure from the biomimetic aspect and could possess excellent biological properties and enhanced stem cell functions ( Wang et al, 2018 ; Bunz et al, 2022 ; Celesti et al, 2022 ). Our results showed that the Cu-TiO 2 group with biomimetic micro/nanostructure was more hydrophilic.…”

First-Principles Computational Study of the Modification Mechanism of Graphene/Graphene Oxide on Hydroxyapatite

“…As an important component of human bone, HA is well known in the field of biomaterial as having good biocompatibility and biological activity [ 1 , 2 , 3 ]. It can produce violent chemical bonds with human bone and promote the growth of new bone.…”