Human gingival fibroblasts express functional chemokine receptor CXCR6

Hosokawa, Yoshitaka; Hosokawa, Ikuko; Ozaki, Kazumi; Nakae, Hajime; Matsuo, Takashi

doi:10.1111/j.1365-2249.2009.03915.x

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…Also in line with us CXCR6 expression was reported as absent on gingiva fibroblasts and in gingiva tissue derived from clinically healthy patients (Hosokawa, Hosokawa, Ozaki, Nakae, & Matsuo, ). However, in a later study the same authors did detect CXCR6 expression and found that its ligand CXCL16 could induce gingiva fibroblast proliferation (Hosokawa, Hosokawa, Ozaki, Nakae, & Matsuo, ). These contradictory results may possibly be explained by different culture conditions and the inflammatory state of the gingiva tissue in the different studies.…”

Section: Discussionmentioning

confidence: 97%

Stimulation of oral fibroblast chemokine receptors identifies CCR3 and CCR4 as potential wound healing targets

Buskermolen

Roffel

Gibbs

2017

Journal Cellular Physiology

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The focus of this study was to determine which chemokine receptors are present on oral fibroblasts and whether these receptors influence proliferation, migration, and/or the release of wound healing mediators. This information may provide insight into the superior wound healing characteristics of the oral mucosa. The gingiva fibroblasts expressed 12 different chemokine receptors (CCR3, CCR4, CCR6, CCR9, CCR10, CXCR1, CXCR2, CXCR4, CXCR5, CXCR7, CX3CR1, and XCR1), as analyzed by flow cytometry. Fourteen corresponding chemokines (CCL5, CCL15, CCL20, CCL22, CCL25, CCL27, CCL28, CXCL1, CXCL8, CXCL11, CXCL12, CXCL13, CX3CL1, and XCL1) were used to study the activation of these receptors on gingiva fibroblasts. Twelve of these fourteen chemokines stimulated gingiva fibroblast migration (all except for CXCL8 and CXCL12). Five of the chemokines stimulated proliferation (CCL5/CCR3, CCL15/CCR3, CCL22/CCR4, CCL28/CCR3/CCR10, and XCL1/XCR1). Furthermore, CCL28/CCR3/CCR10 and CCL22/CCR4 stimulation increased IL‐6 secretion and CCL28/CCR3/CCR10 together with CCL27/CCR10 upregulated HGF secretion. Moreover, TIMP‐1 secretion was reduced by CCL15/CCR3. In conclusion, this in‐vitro study identifies chemokine receptor‐ligand pairs which may be used in future targeted wound healing strategies. In particular, we identified the chemokine receptors CCR3 and CCR4, and the mucosa specific chemokine CCL28, as having an predominant role in oral wound healing by increasing human gingiva fibroblast proliferation, migration, and the secretion of IL‐6 and HGF and reducing the secretion of TIMP‐1.

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Section: Discussionmentioning

confidence: 97%

Stimulation of oral fibroblast chemokine receptors identifies CCR3 and CCR4 as potential wound healing targets

Buskermolen

Roffel

Gibbs

2017

Journal Cellular Physiology

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“…Further, TNF‐ α and IFN‐ γ enhanced ICAM‐1 and vascular cell adhesion molecule 1 expression in HGFs, and IL‐1 β upregulated ICAM‐1 expression (Hosokawa et al 2006). IL‐1 β , TNF‐ α and IFN‐ γ also increased expression of CXCL16 in HGFs with synergism between IFN‐ γ and IL‐1 β (Hosokawa et al 2007), and HGFs also expressed CXCR6, the receptor for CXCL16 (Hosokawa et al 2009b). A combination of TNF‐ α and IL‐4/IL‐13 increased CCL17 expression (Hosokawa et al 2008).…”

Section: Part 3: Cytokine Interactions and Immune Regulation In Periomentioning

confidence: 99%

How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis?

Preshaw

Taylor

2011

J Clinic Periodontology

343

315

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Objective: To review current knowledge on cytokine interactions and the cytokinemediated links between innate and adaptive immunity that are relevant to the pathophysiology of periodontitis. Materials and Methods: A structured review of the literature was undertaken to identify relevant research publications using a Medline search from 1950 to September 2010. The focus of the search was on the functional role of cytokines, i.e. their actions and responses relevant to the pathogenesis of periodontal disease rather than more descriptive studies of their expression in tissues and body fluids. It was not possible to conduct a traditional systematic review with a focussed question due to the heterogeneity of published research. Results: There is enormous heterogeneity in the periodontal literature in terms of experimental approaches. We have the deepest understanding of the role of the proinflammatory cytokines [e.g. interleukin (IL)-1b, tumour necrosis factor-a, IL-6] with accumulating data on T-cell regulatory cytokines (e.g. IL-12, IL-18), chemokines and cytokines which mediate bone cell development and function (e.g. receptor activator of NFkB ligand, osteoprotegerin). It is clear that there are multiple, overlapping and complex functional links between cytokines with regulatory control exerted at a number of levels and involving numerous cell types (both immune cells and resident cells in the periodontium). Conclusion: Cytokines appear to interact functionally in networks in the periodontium and integrate aspects of innate and adaptive immunity. However, our understanding is far from complete, particularly how molecular and cellular pathways relate to disease pathogenesis. We should adopt consistent experimental approaches to gain better insight into the totality of cytokine networks and how they drive immune responses in the periodontium.

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“…6A). These signaling pathways, including p38 and ERK, are also involved in the expression of MMP-9 (34,43), CXCR4 and CXCR6 (44,45). In addition, previous studies have revealed that F-actin re-arrangement is also regulated by the p38 or ERK signaling pathways (46,47).…”

Section: Discussionmentioning

confidence: 98%

Effect of Sorbus commixta on the invasion and migration of human hepatocellular carcinoma Hep3B cells

Park

Chung

et al. 2017

International Journal of Molecular Medicine

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Tumor metastasis is a main cause of cancer-related morbidity and mortality. Thus, a number of medicinal herbs and phytochemicals have been investigated as possible candidates for the inhibition of cancer metastasis. Sorbus commixta Hedl. (SC) is a traditional medicinal plant used in the treatment of inflammatory diseases, as it has antioxidant, anti-inflammatory, anti-atherosclerotic and anti-hepatotoxic activities. In this study, we demonstrate that the water extract of SC exerts inhibitory effect on the invasion and migration of hepatocellular carcinoma Hep3B cells. The activity and expression of matrix metalloproteinase (MMP)-9, which is responsible for the invasion of cancer cells, was decreased by SC treatment. The invasive and migratory potentials of the Hep3B cells were also decreased, as evidence by in vitro assay using the Boyden chamber system. In addition, the expression of the chemokine receptors, C-X-C chemokine receptor type 4 (CXCR)4 and C-X-C chemokine receptor type 6 (CXCR6), were inhibited by SC in Hep3B cells. Furthermore, actin fiber organization was markedly suppressed by SC treatment. Taken together, the findings of this study suggest for the first time, to the best of our knowledge, that SC suppresses the invasion and migration of highly metastatic Hep3B cells.

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Human gingival fibroblasts express functional chemokine receptor CXCR6

Cited by 8 publications

References 17 publications

Stimulation of oral fibroblast chemokine receptors identifies CCR3 and CCR4 as potential wound healing targets

Stimulation of oral fibroblast chemokine receptors identifies CCR3 and CCR4 as potential wound healing targets

How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis?

Effect of Sorbus commixta on the invasion and migration of human hepatocellular carcinoma Hep3B cells

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