Human growth hormone (hGH) in urine and its correlation to serum hGH examined by a highly sensitive sandwich enzyme immunoassay

Hashida, Seiichi; Ishikawa, Eiji; Kato, Yuzuru; Imura, Hiroo; Mohri, Zen‐Ichi; Murakami, Yoshiaki

doi:10.1016/0009-8981(87)90455-4

Cited by 62 publications

(33 citation statements)

References 9 publications

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“…There is considerable day to day variation; therefore the mean of three GH concentrations divided by creatinine was used as an index of endogenous GH secretion. Urinary GH was measured by a sandwich enzyme immunoassay (12,13), and the coefficient of variation defined over the range 3.4-36 ng/l. Intra-assay and interassay variations of this measurement were 6-8 and 9-10% respectively.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Age-dependent changes in body composition in postmenopausal Japanese women: relationship to growth hormone secretion as well as serum levels of insulin-like growth factor (IGF)-I and IGF-binding protein-3

Sugimoto

Nakaoka²,

Nasu³

et al. 1998

European Journal of Endocrinology

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The present study was performed to investigate the age-dependent changes in body composition and the possible role of growth hormone (GH), insulin-like growth factor (IGF)-I and IGF-binding protein-3 (IGFBP-3) in these changes in postmenopausal Japanese women. A total of 161 Japanese women aged 45-88 years (mean 62) were enrolled in the cross-sectional study. Body composition (bone mineral content (BMC), lean body mass (LBM) and fat) was measured by dual-energy X-ray absorptiometry, and the percentage of BMC, LBM and fat was calculated by dividing each absolute value of body composition by total body mass. Urinary GH concentration divided by creatinine in nocturnal urine samples collected just after waking was used as an index of endogenous GH secretion. Serum levels of IGF-I and IGFBP-3 were measured by RIA. Urinary GH levels as well as serum levels of IGF-I and IGFBP-3 declined with age. BMC, %BMC and LBM also declined with age, while fat mass and %fat did not obviously change with age. Urinary GH levels as well as serum levels of IGF-I and IGFBP-3 correlated positively with BMC, even if age was taken into account. On the other hand, urinary GH correlated negatively with fat and %fat. In contrast, serum levels of IGF-I and IGFBP-3 correlated positively with fat and %fat. LBM did not correlate with either urinary GH or serum IGFBP-3 levels but exhibited a weakly positive correlation with serum IGF-I level. The present study suggests that the GH-IGF-I-IGFBP-3 axis positively regulates bone mass, and that GH and IGF-I-IGFBP-3 inversely regulate fat mass, i.e. GH negatively and IGF-I-IGFBP-3 positively regulates it.

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Section: Biochemical Measurementsmentioning

confidence: 99%

Age-dependent changes in body composition in postmenopausal Japanese women: relationship to growth hormone secretion as well as serum levels of insulin-like growth factor (IGF)-I and IGF-binding protein-3

Sugimoto

Nakaoka²,

Nasu³

et al. 1998

European Journal of Endocrinology

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“…Spontaneous GH secretion is known to be pulsatile and the GH concentration in a single blood sample may not accurately reflect endogenous GH secretion. It has been recently reported that assay of the urinary GH concentration by a highly-sensitive enzyme immunoassay is useful in evaluating endogenous GH secretion in normal subjects, GH-deficient patients, and acromegalic patients [10][11][12][13]. Our data suggest that thyroid hormone stimulates urinary GH secretion which reflect endogenous GH secretion.…”

Section: Discussionmentioning

confidence: 51%

“…Recently, several reports have revealed that urinary GH determinations by means of sandwich enzyme immunoassay are useful in evaluating endogenous GH secretion in normal and GH-deficient subjects [10][11][12][13]. Therefore, it is important to reevaluate the relationship between thyroid function and GH secretion by this method.…”

Section: Introductionmentioning

confidence: 99%

“…Serum TSH was measured with a commercial immunoradiometric assay kit (Dainabot). Urinary GH levels were determined by a highly-sensitive sandwich enzyme immunoassay kit (Sumitomo, Osaka, Japan) as previously described [10][11]. Briefly, dialyzed urine samples were incubated with anti-hGH IgG-coated polystyrene balls at 37°C for 6 h. The balls were then incubated with affinity-purified-anti-hGH F (ab')z conjugated with horseradish peroxidase at 4°C for 16 h and at 20°C for 6 h. The activity of peroxidase bound to the balls was assayed by fluorometry with 3-(p-hydroxyphenyl) propionic acid as the substrate.…”

Section: Introductionmentioning

confidence: 99%

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Relationship between Thyroid Functions and Urinary Growth Hormone Secretion in Patients with Hyper- and Hypothyroidism.

et al. 1994

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Abstract.Thyroid hormone plays an important role in growth hormone (GH) synthesis and secretion. To study the relationship between thyroid function and urinary GH secretion in the hyperthyroid and hypothyroid states, we measured thyroid hormones, simultaneously with serum and urinary GH levels, in 54 patients with thyroid diseases.GH-releasing hormone (GRH) test was performed in 18 patients in order to evaluate serum and urinary GH responses to GRH in hyper-and hypothyroid states. Serum thyroid hormone levels were strongly correlated with the urinary GH levels in the patients, and the correlation was greater than that between serum thyroid hormone and serum GH levels. Urinary GH levels were significantly higher in the hyperthyroid patients than in the euthyroid and hypothyroid patients, although serum GH levels were not significantly different among these three groups. Serum GH response to GRH was significantly decreased in hyperthyroid patients as compared to euthyroid patients. However, urinary GH levels after GRH administration were not decreased in the hyperthyroid patients.These results suggest that hyperthyroid states increase GH in urine and may accelerate the urinary clearance of GH.

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“…We previously developed a highly sensitive ELISA for urinary growth hormone (GH) [Hashida, 1987, Sukegawa, 1988, which showed that assay of GH during nocturia was useful as a supporting diagnostic index of the pituitary dwarf. We have also recently developed an ultra-sensitive ICT-EIA for urinary soluble human insulin receptor ectodomain (sIR) and found that urinary sIR levels correlated well with sIR blood levels [Umehara, 2009].…”

Section: Discussionmentioning

confidence: 99%

Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

Hashida¹,

Miyzawa²,

Hirajima³

et al. 2012

Trends in Immunolabelled and Related Techniques

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Human growth hormone (hGH) in urine and its correlation to serum hGH examined by a highly sensitive sandwich enzyme immunoassay

Cited by 62 publications

References 9 publications

Age-dependent changes in body composition in postmenopausal Japanese women: relationship to growth hormone secretion as well as serum levels of insulin-like growth factor (IGF)-I and IGF-binding protein-3

Age-dependent changes in body composition in postmenopausal Japanese women: relationship to growth hormone secretion as well as serum levels of insulin-like growth factor (IGF)-I and IGF-binding protein-3

Relationship between Thyroid Functions and Urinary Growth Hormone Secretion in Patients with Hyper- and Hypothyroidism.

Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

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