Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: A review

Ben, Yujie; Fu, Caixia; Hu, Min; Liu, Lei; Wong, Ming Hung; Chen, Kewei

doi:10.1016/j.envres.2018.11.040

Cited by 908 publications

(365 citation statements)

References 91 publications

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“…However, the ratio of antibiotic-resistant bacteria is not directly related to the concentration of the antibiotic in food. Because the concentration of florfenicol in food is as high as 0.09 μg/ml while the concentration of cephalexin in food is much lower (0.016 μg/ml; Ben et al, 2019). In contrast, the abundance of Ceph R is the highest.…”

Section: Resultsmentioning

confidence: 99%

“…In the anaerobic tubes, the samples were diluted 1:10 with phosphate buffer saline (PBS) solution or supplemented with 40% D 2 O (v/v) and various concentrations of amoxicillin, cephalexin, florfenicol, tetracycline and vancomycin (Table 1, Sigma-Aldrich). The three antibiotic concentrations represent the typical concentration in food (Ben et al, 2019), the minimum inhibitory concentration (MIC) and two times the MIC (2 × MIC), respectively. The MIC was determined according to the averaged MIC of commensal gut bacteria in the Eucast data (http://mic.eucast.org/ Eucast2/; Rettedal et al, 2014).…”

Section: Human Faeces Collection and Bacterial Growth Conditionsmentioning

confidence: 99%

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Raman‐activated sorting of antibiotic‐resistant bacteria in human gut microbiota

Wang

Kong

et al. 2020

Environmental Microbiology

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Summary The antibiotic‐resistant bacteria (ARB) and antibiotic‐resistant genes (ARGs) in human gut microbiota have significant impact on human health. While high throughput metagenomic sequencing reveals genotypes of microbial communities, the functionality, phenotype and heterogeneity of human gut microbiota are still elusive. In this study, we applied Raman microscopy and deuterium isotope probing (Raman–DIP) to detect metabolic active ARB (MA‐ARB) in situ at the single‐cell level in human gut microbiota from two healthy adults. We analysed the relative abundances of MA‐ARB under different concentrations of amoxicillin, cephalexin, tetracycline, florfenicol and vancomycin. To establish the link between phenotypes and genotypes of the MA‐ARB, Raman‐activated cell sorting (RACS) was used to sort MA‐ARB from human gut microbiota, and mini‐metagenomic DNA of the sorted bacteria was amplified, sequenced and analysed. The sorted MA‐ARB and their associated ARGs were identified. Our results suggest a strong relation between ARB in human gut microbiota and personal medical history. This study demonstrates that the toolkit of Raman–DIP, RACS and DNA sequencing can be useful to unravel both phenotypes and genotypes of ARB in human gut microbiota at the single‐cell level.

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Section: Resultsmentioning

confidence: 99%

Section: Human Faeces Collection and Bacterial Growth Conditionsmentioning

confidence: 99%

Raman‐activated sorting of antibiotic‐resistant bacteria in human gut microbiota

Wang

Kong

et al. 2020

Environmental Microbiology

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“…Terjadinya ketidakseimbangan mikrobiota, dapat mengarah pada terjadinya proliferasi dari bakteri berbahaya dan patogen oportunistik, lebih jauh lagi dapat menyebabkan berbagai macam penyakit seperti pseudomembranous colitis, kelainan intestin dan kanker colorektal. Kejadian yang lebih buruk yaitu apabila bakteri dalam usus telah resisten, kemudian berkembang biak dengna jumlah yang cukup banyak, maka penyakitpenyakit yang di sebabkan karena bakteri tersebut akan dapat menyebabkan kematian karena tidak dapat diobati (Ben, 2018).…”

Section: Metode Penelitianunclassified

Deteksi Residu Antibiotik Oksitetrasiklin pada Susu Kambing Peranakan Etawah di Kelurahan Kalipuro, Banyuwangi dengan Uji Bioassay

et al. 2019

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Oksitetrasiklin diketahui sebagai salah satu antibiotik yang sering digunakan dalam menangani kasus mastitis pada hewan ternak. Penelitian ini merupakan penelitian deskriptif untuk mengetahui ada atau tidaknya residu oksitetrasiklin pada susu kambing Peranakan Etawah (PE) di Kelurahan Kalipuro, Banyuwangi. Sampel susu di koleksi kemudian dilakukan pengujian residu antibiotik dengan metode uji bioassay menggunakan bakteri standar Bacillus cereus ATCC 11778. Penelitian yang dilakukan pada 26 sampel susu yang di ambil secara acak berasal dari ternak kambing perah PE di dapatkan hasil yang negatif. Hal ini di tunjukkan dengan tidak terbentuknya zona hambat di sekitar well yang diisi sampel susu. Berbeda dengan kontrol postif oksitetrasiklin dengan konsentrasi 1 µg/ml yang menghasilkan zona hambatan sekitar 18 mm. Dapat disimpulkan bahwa 100% dari 26 jumlah sampel susu kambing PE yang di ambil secara acak dan di periksa dari Kelurahan Kalipuro, Banyuwangi tidak mengandung residu antibiotik oksitetrasiklin.

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“…Nowadays the extensively used antibiotics are considered as toxic emerging contaminants, which pose severe threats to environmental ecosystems [1]. Humans are directly and indirectly exposed to different antibiotics cocktail through inhalation, ingestion of drugs, drinking water, and foods, and their risk assessments have attracted more attention, recently [2][3][4]. As a result, antibiotics may progressively enter into the human gastrointestinal tract and produce a large variety of antibiotic-resistant gut-micro ora [5].…”

Section: Introductionmentioning

confidence: 99%

Human gut microbiota evaluation and comparation after the separate or combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

Liu¹,

Das²,

Suo³

et al. 2020

Preprint

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Background A cocktail of drugs is an emerging toxic contaminant that has potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics cocktail on human intestinal microbiota dysbiosis and related health risk are not fully understood. Therefore, for the first time, this study evaluated and compared the in vitro ability of amoxicillin (AMX) and polymyxin E (POL) used separately or combined on antibiotic resistance genes (ARGs) as well as human disease-related pathways in the simulated human gut. Results This study indicated that the combination exposure of POL with AMX reduced the occurrence of drug resistance in the gut microbiota caused by single antibiotic treatment. However, in comparison with the separate use of AMX and POL, the combined treatment exhibited a significantly higher ability to increase the human disease-related pathways. The combination effects on genetic level might attribute to microbiota shift, as co-occurrence patterns suggested that Bifidobacterium attributed to ARGs increasing in the POL treatment group and Enterobacter played a crucial role in human disease-related pathways enrichment after combination treatment. Conclusion These results may open up new perspectives for assessing the direct effects of combination antibiotics on the intestinal microbiota. These suggested side-effects should be considered for a combination of antibiotics prescription.

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Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: A review

Cited by 908 publications

References 91 publications

Raman‐activated sorting of antibiotic‐resistant bacteria in human gut microbiota

Raman‐activated sorting of antibiotic‐resistant bacteria in human gut microbiota

Deteksi Residu Antibiotik Oksitetrasiklin pada Susu Kambing Peranakan Etawah di Kelurahan Kalipuro, Banyuwangi dengan Uji Bioassay

Human gut microbiota evaluation and comparation after the separate or combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

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