Human Immunodeficiency Virus p24 Antigen Testing in Cornea Donors

Chung, Christine W.; Rapuano, Christopher J.; Laibson, Peter R.; Lytle, Robert E.; Quirk, J.; Cohen, Elisabeth J.

doi:10.1097/00003226-200104000-00008

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…False-reactive virological results had been reported for donor samples taken post mortem. [ 22 ] Avoiding false-reactive virological results is an important factor in current cornea graft scarcity. [ 4 , 5 ]…”

Section: Discussionmentioning

confidence: 99%

Factors influencing the virological testing of cornea donors

et al. 2017

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To assess the influence of donor, environment, and logistical factors on the results of virological testing of blood samples from cornea donors.Data from 670 consecutive cornea donors were analyzed retrospectively. Logistic regression analysis was used to assess the influence of different factors on the results of virological testing of blood samples from cornea donors.The mean annual rate of donors with serology-reactive or not evaluable result was 14.8% (99 of 670) (range 11.9%–16.9%). The cause of donor death by cancer increased the risk of serology-reactive or not evaluable result (P = .0300). Prolonged time between death and post mortem blood removal was associated with a higher rate of serology-reactive or not evaluable result (P < .0001). Mean monthly temperature including warmer months, differentiating between septic and aseptic donors, sex, and donor age had no significant impact on the results of virological testing of blood samples from cornea donors.The cause of donor death by cancer and a prolonged time between death and post mortem blood removal seem to be mainly responsible for serology-reactive or not evaluable result of blood samples from cornea donors. The percentage of discarded corneas caused by serology-reactive or not evaluable result may be reduced by shortening the period of time between death and post mortem blood removal.

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Section: Discussionmentioning

confidence: 99%

Factors influencing the virological testing of cornea donors

et al. 2017

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“…However, there is generally only limited scope for improvement in this area. Over the years the criticality of sample quality has been highlighted by others with issues such as haemolysis, haemodilution and volume identified [9–13]. NHSBT has spent time looking at the training of its retrieval teams in relation to obtaining optimal blood samples, but these may be collected separately to the tissue retrieval, and by local staff.…”

Section: Discussionmentioning

confidence: 99%

The serological screening of deceased tissue donors within the English Blood Service for infectious agents - a review of current outcomes and a more effective strategy for the future

Kitchen

Gillan

2010

Vox Sanguinis

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Background and Objectives The overall effectiveness of the NHSBT screening programme for infectious agents in deceased tissue donors is examined and evaluated in terms of current outcomes and how to improve upon these outcomes. Materials and MethodsThe screening results and any subsequent confirmatory results from a total of 1659 samples from NHSBT deceased donors referred to NTMRL for screening for infectious agents were included in the analysis.Results Overall 1566 ⁄ 1659 (94AE4%) of the samples were screen negative. A total of 93 were repeat reactive on screening for one or more of the mandatory markers screened for, of which only 12 (13%) were subsequently confirmed to be positive on confirmatory testing. The majority of the repeat reactive samples were demonstrating non-specific reactivity with the screening assays in use.Conclusion Overall, the NHSBT screening programme for infectious agents in deceased tissue donors is very effective with a relatively low overall loss of donors because of non-specific reactivity. However, unnecessary loss of tissue products is not acceptable, and although this programme compares favourably with the outcomes of other such programmes, the confirmatory results obtained demonstrate both the need and the potential for improving the outcomes. This is particularly important as one donor may donate more than one product, and can be achieved very easily with a change to the screening algorithm followed, using the confirmatory data obtained to support and validate this change.Contents Summary Critical analysis of the NHSBT screening programme for infectious agents in deceased tissue donors and a strategy involving the design and use of a different screening algorithm to improve these outcomes.

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“…Although p24 testing of cornea donors has not been recommended as mandatory, many tissue banks using other organs from cornea donors do perform this assay and the US FDA requires that eye banks reject corneal tissue if a reactive p24 assay is reported. The use of the p24 assay in the screening of cornea donors may result in excessive waste of donor tissue [28] but adoption of HIV p24 antigen testing may be considered as part of serology testing in developing nations where HIV infections have been on a steady rise in the recent years.…”

Section: Selection Of Donor Corneamentioning

confidence: 99%

Challenges of eye banking in a developing world

Vanathi¹,

Tandon

Panda

et al. 2007

Expert Review of Ophthalmology

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Eye donation is an act of supreme kindness to humanity and the society is obliged to bring honor to the memory of all eye donors for this truly philanthropic gift. Understanding the functioning of eye banks, donor criteria and the process of eye donation is the basis for facing challenges within eye banking in the developing world. The number of banks and their organizational level, methods of explantation, processing and preservation, quality standards, and new developments will have to be reviewed and regulated regularly. Efforts towards improvement in eye banking will ensure higher standards of both the quality and quantity of corneal tissue for rehabilitation of corneal blind patients. Effective state legislation is required to meet a society's ophthalmic surgical needs in transplantation programs. Incorporating advanced tissue-engineering services into the scope of the functions of an eye bank will make such higher end facilities available to routine corneal surgeons, thereby raising the standard of eye banking to greater heights.Expert Rev. Ophthalmol. 2(6), 923-930 (2007)

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Human Immunodeficiency Virus p24 Antigen Testing in Cornea Donors

Cited by 8 publications

References 17 publications

Factors influencing the virological testing of cornea donors

Factors influencing the virological testing of cornea donors

The serological screening of deceased tissue donors within the English Blood Service for infectious agents - a review of current outcomes and a more effective strategy for the future

Challenges of eye banking in a developing world

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