Human Intestinal VIP Receptor: Cloning and Functional Expression of Two cDNA Encoding Proteins with Different N-Terminal Domains

Activation of the vasoactive intestinal peptide 2 receptor modulates normal and atrophying skeletal muscle mass and force

“…To evaluate the functionality of the VIP receptors expressed in skeletal muscle and to validate the observation that only VPAC2R is expressed in skeletal muscle, skeletal muscles in organ bath were stimulated with either the VPACR-nonselective agonist VIP, the VPAC1R-selective agonist [K 15 ,R (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27), or the VPAC2R-selective agonist Ro-25-1553, and cAMP generation was evaluated. As can be seen in Fig.…”

“…Both rodent and human VPAC1R and VPAC2R have been cloned, with splice variants of each receptor demonstrating unique tissue expression distributions (1,8,13,16,20,21,28,38,39,41,44). VPAC1R and VPAC2R belong to the G protein-coupled receptor class and are positively coupled to G␣s.…”

Absence of functional receptors for parathyroid hormone and parathyroid hormone-related peptide in Blomstrand chondrodysplasia.

“…A vector permitting expression of the wild-type PTH/PTHrP receptor with an epitope Tag (19) added at the COOH-terminal end (PTH-R/wt) was obtained by ligating the dodecapeptide sequence coding for the Tag marker to the COOH-terminal end of the full-length PTH/PTHrP receptor cDNA, and subcloning the fragment into pcDNA1-Amp (Invitrogen, La Jolla, CA). The functional properties of the tagged and native receptors were indistinguishable (data not shown).…”

Mack Forster Award Lecture Receptor nuclear medicine: vasointestinal peptide and somatostatin receptor scintigraphy for diagnosis and treatment of tumour patients

“…Molecular cloning of human SSTR and VIPR has recently provided new insights into the biology and interactions of VIP and SST/OCT. So far, five different human SSTRs and two different VIPRs have been characterized in detail and have been cloned [37][38][39][40][41][42][43][44][45][46][47][48][49][50]. More recent data have demonstrated that SSTR3 is responsible for binding both SST/OCT and VIP and the observed cross-competition between these peptides in primary human tumours as well as human immortalized tumours [21].…”