Human islet transplantation from pancreases with prolonged cold ischemia using additional preservation by the two-layer (UW solution/perfluorochemical) cold-storage method

Tsujimura, Takuya; Kuroda, Yoshikazu; Kin, Tatsuya; Ávila, José G.; Rajotte, Ray V.; Korbutt, Gregory S.; Ryan, Edmond A.; Shapiro, A. M. James; Lakey, Jonathan R. T.

doi:10.1097/00007890-200212270-00007

Cited by 114 publications

(100 citation statements)

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“…Matsumoto et al (26) showed that human islet function, using an in vivo nude mouse transplant bioassay, have resulted in a 67% islet isolation success rate despite the use of a remote islet isolation center (22). Tsujimura was the same between two BMI groups; group 1 were BMI of 30 or more and group 2 were BMI of less than et al (43,44) reported that the overall islet recovery from pancreas preserved using two-layer method is signifi-30. However, Benhamou et al…”

Section: Influence Of Donor-related Parameters On the In Vivo Functiomentioning

confidence: 75%

The Effect of Isolation Methods and the Use of Different Enzymes on Islet Yield and In Vivo Function

et al. 2008

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The ability to isolate high-yield pure and viable islets from human cadaver pancreas donors is dependent on donor factor as well as isolation factors. The aim of this study was to examine factors influencing islets recovery and in vivo function with an emphasis on donor and isolation methods as well as to compare the effectiveness of Liberase, widely used in clinical islet isolation, with Serva for the isolation of pure functional islets. The results of 123 islet isolations using Liberase for digestion were compared with those of 113 isolations with Serva. Islet equivalents per gram of tissue were similar between Liberase and Serva (3620 ± 1858 vs. 4132 ± 2104, p < 0.2) as well as the percent purity (75 ± 16 vs. 74 ± 15, p < 0.9). In vivo function of islets from 71 isolations (Liberase = 45, Serva = 26) were further tested by transplantation into NOD-SCID mice following short-term culture (<6 days, n = 71). Our data show that both Liberase-and Serva-isolated islets showed similar function results following short-term culture. These data demonstrate that there is no difference in islet yield, purity, and function between the two enzymes. However, when these 71 isolations were analyzed for in vivo function with emphasis on donor factors, cold ischemia time (12.0 ± 5.3 vs. 15.0 ± 5.7, p < 0.04), islet integrity (1.6 ± 0.7 vs. 1.3 ± 0.5, p < 0.05), and female gender were the only factors that correlated with in vivo function. We also compared the mechanical-shaking method for islets isolation with hand-shaking methods. Our results show that although there is no different in islet yield, purity, and integrity between different enzymes using the same method, hand-shaking method yields more islets with better integrity than mechanical-shaking method.

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Section: Influence Of Donor-related Parameters On the In Vivo Functiomentioning

confidence: 75%

The Effect of Isolation Methods and the Use of Different Enzymes on Islet Yield and In Vivo Function

et al. 2008

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“…Nevertheless, inspite of prolonged cold ischemia time significantly more islets could be isolated and recovered after culture compared to storage in UWS (Matsumoto et al, 2002a). These promising results could be confirmed in a similar setting by achieving a significantly higher islet transplantation rate of 71% in pancreases that were oxygenated for additionally 3 hours compared to UWS storage alone (36%) (Tsujimura et al, 2002a). An even more impressing improvement of the success rate from 11% to 53% was obtained in TLM-oxygenated pancreases from marginal donors older than 50 years (Ricordi et al, 2003).…”

Section: Pancreas Oxygenation For Subsequent Islet Transplantationmentioning

confidence: 82%

“…As reported, this was the case for the studies from the Edmonton group (Tsujimura et al, 2002a, Tsujimura et al, 2004a, Salehi et al, 2006, Kin et al, 2006a but not for the study from the Nordic Network (Caballero-Corbalan et al, 2007). This is of particular relevance when pancreases from obese donors are recovered.…”

Section: Pancreas Oxygenation For Subsequent Islet Transplantationmentioning

confidence: 86%

“…One important difference that exists between the larger trials and previously published smaller studies concerns the method of oxygenation used. While in the smaller trials pancreases had been subjected to continuous oxygenation for a short period of approximately 3 hours subsequent to cold storage (Tsujimura et al, 2002a, Tsujimura et al, 2004a, Salehi et al, 2006, the large scale trials utilized oxygen-precharged PFD for the entire period of cold ischemia. However, experiments in rats clearly suggested that oxygen-precharged PFD is equivalent to continuously oxygenated PFD with regard to pancreas ATP concentration and isolated islet yield after 24 hours of cold storage (Hiraoka et al, 2001).…”

Section: Pancreas Oxygenation For Subsequent Islet Transplantationmentioning

confidence: 99%

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Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

Brandhorst¹,

Johnson²,

Brandhorst³

2012

Organ Donation and Transplantation - Public Policy and Clinical Perspectives

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“…[36][37][38] Hypothermia has also been used to add to the preservation of the organs in transplantation, even in the case of pancreas transplantation, on account of decreased organ metabolism. 39,40 Several experimental studies showed an attenuation in ischemia and reperfusion injury, in different organs (liver, lung, neuron), with hypothermia in different degrees. [17][18][19][20][21] Hypothermia was also used as a stimulating factor for the production of heat shock proteins, that showed a prophylactic protection of organ lesions including ischemia and reperfusion injury and acute pancreatitis.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Is Enhanced by Hypothermia Imposed on Cerulein-Induced Pancreatitis in Rats

Andraus

Jukemura

Dutra

et al. 2007

Clinics

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MC. Oxidative stress is enhanced by hypothermia imposed on cerulein-induced pancreatitis in rats. Clinics. 2007;62(4): 483-90. BACKGROUND:Hypothermia is a frequent event in severe acute pancreatitis (AP) and its real effects on the normal pancreas have not been well demonstrated. Moreover, neither have its effects on the outcome of acute pancreatitis been fully investigated. One hypothesis is that oxidative stress may be implicated in lesions caused or treated by hypothermia. Aim of the study: To investigate the effect of hypothermia in cerulein-induced acute pancreatitis (CIAP) in rats and the role played by oxidative stress in this process. METHODS: Male Wistar rats were divided into hypothermic and normothermic groups. Hypothermia was induced with a cold mattress and rectal temperature was kept at 30 o C for one hour. Acute pancreatitis was induced with 2 doses of cerulein (20 ìg/kg) administered at a one-hour interval. Serum amylase, pancreas vascular permeability by Evan's blue method, pancreas wet-to-dry weight ratio and histopathology were analyzed in each group. RESULTS: When compared with normothermic rats, hypothermic animals, with cerulein-induced acute pancreatitis, showed higher levels of pancreatic vascular permeability (P < 0.05), pancreas wet-to-dry weight ratio (P = 0.03), and histologically verified edema (P < 0.05), but similar serum amylase levels. The hypothermic group showed a higher oxidized-reduced glutathione ratio than the normothermic group. CONCLUSION: Moderate hypothermia produced a greater inflammatory response in established acute pancreatitis induced by cerulein in rats. Moreover, this study suggests that oxidative stress may be one of the mechanisms responsible for the worse outcome in hypothermic rats with cerulein-induced acute pancreatitis.

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Human islet transplantation from pancreases with prolonged cold ischemia using additional preservation by the two-layer (UW solution/perfluorochemical) cold-storage method

Cited by 114 publications

References 0 publications

The Effect of Isolation Methods and the Use of Different Enzymes on Islet Yield and In Vivo Function

The Effect of Isolation Methods and the Use of Different Enzymes on Islet Yield and In Vivo Function

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

Oxidative Stress Is Enhanced by Hypothermia Imposed on Cerulein-Induced Pancreatitis in Rats

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