Human leucocyte antigen-Bw4 and Gag-specific T cell responses are associated with slow disease progression in HIV-1B-infected anti-retroviral therapy-naive Chinese

Li, W.-H.; C.-Y., Li,; Yang, Hongbing; Zhang, H.-P.; Zhang, X.; Kong, Lu; Xu, Xiao-Ning; Lu, Shichun; Hu, Yan

doi:10.1111/cei.12025

Cited by 1 publication

(2 citation statements)

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“…About 38% peptides were recognized in Gag protein. 16 However, the overlap polypeptide in the Nef core region caused a higher immune response (90%) compared with the Gag region, leading to response in 62.1% individuals. These results suggested that the ability to target Nef epitopes with sufficient strength might serve as a correlate of protection.…”

Section: Discussionmentioning

confidence: 99%

“…The peptides were synthesized by Sigma and the Medical Research Council Human Immunology Unit (WIMM, Oxford, UK); 18-mer peptides overlapped by 10 nucleotides and representing the consensus clade B proteome were pooled into the Nef core protein ( Table 2 Nef Core OLPs sequence, magnitude and breath), as described previously. 8,9,16 HLA typing…”

Section: Plasma Vl and Cd4 C T-cell Countsmentioning

confidence: 99%

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HLA-DQB1*06 and breadth of Nef core region-specific T-cell response are associated with slow disease progression in antiretroviral therapy-naive Chinese HIV-1 subtype B patients

Xia

et al. 2017

Human Vaccines & Immunotherapeutics

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Vaccines still are an important way to prevent and treat acquired immunodeficiency syndrome (AIDS). For developing an effective T cell-based AIDS vaccine, it is critical to define the human leukocyte antigen (HLA) type and epitope that elicit the most potent responses. This study involved 29 antiretroviral therapy-naive and chronic human immunodeficiency virus (HIV)-1 subtype B-infected individuals. A polymerase chain reaction-sequence-specific primer was used to detect the HLA typing, and the enzyme-linked immunospot assay to quantify the T-cell immune function. The results showed that the HLA-DQB1*06-positive group had higher CD4 counts and lower viral load (VL) compared with the HLA-DQB1*06-negative group; A higher magnitude of HIV-1-specific T-cell response and breadth were observed in the HLA-DQB1*06-positive group; the T-cell response was proportional to VL (R = 0.488, P = 0.0368) in the HLA-DQB1*06-positive group. The total T-cell responses to HIV-1 Nef core region were quantified at the single-peptide level. Nine (90%) peptides were recognized in 18 (62.1%) individuals. The breath of Nef core region-specific T-cell response was correlated positively with CD4 T cell count and inversely with VL, which improved disease outcomes. These data revealed that HLA-DQB1*06 had a protective effect on the course of HIV-1 and T-cell targeting of certain specific Nef epitopes, contributing to HIV-1 suppression. The results suggested the potential use of HLA-DQB1*06 and Nef core region in HIV-1 T-cell vaccine design.

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Section: Discussionmentioning

confidence: 99%

Section: Plasma Vl and Cd4 C T-cell Countsmentioning

confidence: 99%