Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp

Abstract

The current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the addition of hydrogen peroxide or tert-butyl hydroperoxide. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the antioxidant property of the extract was evaluated in vitro using hydrogen peroxide and nitric oxide radical-scavenging assays. Compared to the vehicle, the fruit pulp ethanol extract did not exhibit a cytotoxic effect on human lymphocytes. Furthermore, the cytotoxicity of hydrogen peroxide and tert-butyl hydroperoxide to human lymphocytes was significantly reduced by fruit extract pretreatment. The extract and ascorbic acid exhibited similar cytoprotective activity (p > 0.05). The fruit pulp extract showed more antioxidant activity than gallic acid in the hydrogen peroxide-scavenging model, while in the nitric oxide-quenching model, the fruit extract and gallic acid showed similar activity. The fruit pulp of D. microcarpum contains potent antioxidant and cell-protective compounds. The use of the fruit pulp of D. microcarpum as a food supplement could rescue cellular oxidative damage responsible for numerous pathologies.

Biological Activity and Chemical Composition of Detarium microcarpum Guill. and Perr—A Systematic Review

“…The extract had IC 50 values of 90 μ g/mL for hydrogen peroxide and 25 μ g/mL for nitric oxide radical scavenging. Hydrogen peroxide was more effectively quenched by the extract (IC 50 = 90 μ g/mL) than gallic acid at p > 0.05, although the two compounds exhibited similar quenching abilities [ 55 ]. The IC 50 value of the essential oil extracted from the leaves was 21.99 μ L, which is the maximum level of radical scavenging activity [ 28 ].…”

Protective effect of leaf extract of Abutilon indicum on DNA damage and peripheral blood lymphocytes in combating the oxidative stress

“…The fresh leaves were clearly washed and dried at room temperature (shades) (25 °C) and powdered. To minimize the degradation of thermolabile compounds, extraction of 25 g of leaf powder was marshaled by maceration in ethanol over 24 h under 25 °C with constant stirring according to Rouamba et al (2018 ). The ethanolic leaf extract was filtered and dried in a vacuum evaporator and stored at 4 °C until further investigation.…”

Activité Antioxydante des Extraits Méthanoliques de Differents Organes de Detarium microcarpum Guill. & Perr.

“…Les fruits jouent un rôle important au niveau nutritionnel selon plusieurs travaux effectués sur leur composition nutritionnelle et thérapeutique (Cavin et al, 2006 ;Felix et al, 2010 ;Rouamba et al, 2017).…”