Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Abstract

Mesenchymal stem/stromal cells (MSCs) from bone marrow (BM) have been used in coculture systems as a feeder layer for promoting the expansion of hematopoietic progenitor cells (HPCs) for hematopoietic cell transplantation. Because BM has some drawbacks, umbilical cord blood (UCB) and placenta (PL) have been proposed as possible alternative sources of MSCs. However, MSCs from UCB and PL sources have not been compared to determine which of these cell populations has the best capacity of promoting hematopoietic expansion. In this study, MSCs from UCB and PL were cultured under the same conditions to compare their capacities to support the expansion of HPCs in vitro. MSCs were cocultured with CD34+CD38−Lin− HPCs in the presence or absence of early acting cytokines. HPC expansion was analyzed through quantification of colony-forming cells (CFCs), long-term culture-initiating cells (LTC-ICs), and CD34+CD38−Lin− cells. MSCs from UCB and PL have similar capacities to increase HPC expansion, and this capacity is similar to that presented by BM-MSCs. Here, we are the first to determine that MSCs from UCB and PL have similar capacities to promote HPC expansion; however, PL is a better alternative source because MSCs can be obtained from a higher proportion of samples.

Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells

“…Comparing the counts of before cultivation (day 0) with after harvest (day 10) in various coculture conditions (UMC, UEC, UC, UM, UE), the folds of expansion of CD34+CD38− cells under UMC and UEC conditions were more than UC, UM, and UE conditions. The similar effects were also observed on other stromal cells derived from the bone marrow, cord tissue, and placenta in other researches [27–30].…”

Tailored Cytokine Optimization for ex vivo Culture Platforms Targeting the Expansion of Human Hematopoietic Stem/Progenitor Cells

“…Indeed, enhanced expansion of HSPC through a co-culture setting with MSC has also been observed in other studies. Beneficial impact compared to traditional liquid suspension has been described concerning cell expansion levels ( da Silva et al, 2010 ; Fajardo-Orduña et al, 2017 ; Darvish et al, 2019 ), but also in what concerns the biological features of the cultured cells, for instances, contributing toward an enhanced migration capability of HSPC ( Alakel et al, 2009 ; Perdomo-Arciniegas and Vernot, 2011 ). Overall, our evaluation of each expansion system after cytokine optimization has provided a more reliable and unbiased view over their genuine production capabilities of a potential expanded HSPC product.…”

Mesenchymal Stromal Cells from the Epidermis and Dermis of Psoriasis Patients: Morphology, Immunophenotype, Differentiation Patterns, and Regulation of T Cell Proliferation

“…This finding may indicate that the presence of inflammatory stimuli stimulates migration or activation of MSCs in the lesional epidermis. MSCs have been shown to secrete trophic factors that favor tissue remodeling through activation of resident stem cells in tissues [30, 32]. In this sense, it is likely that MSCs contribute to the incomplete expansion and differentiation of epidermal stem cells in lesional epidermis, which would result in epidermis thickening [14].…”