1982
DOI: 10.1016/0014-5793(82)80283-4
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Human milk fibronectin: identification of fibronectin fragments by transfer of milk proteins from polyarcrylamide gels to nitrocellulose sheets

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1983
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Cited by 9 publications
(6 citation statements)
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“…FN concentrations determined by ELISA with the monoclonal antibody specific to the cell-binding FN domain (Table 1, Fig. 1A, 0.6-6.1 mg/L, n = 132) were lower than those reported earlier by Zardi et al [1] (1.7-12.2 mg/L; 12 milk samples) and Friss et al [2] (mean = 13.4 mg/L; 5 milk samples) and obtained by the use of less specific polyclonal antibodies.…”
Section: Discussioncontrasting
confidence: 73%
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“…FN concentrations determined by ELISA with the monoclonal antibody specific to the cell-binding FN domain (Table 1, Fig. 1A, 0.6-6.1 mg/L, n = 132) were lower than those reported earlier by Zardi et al [1] (1.7-12.2 mg/L; 12 milk samples) and Friss et al [2] (mean = 13.4 mg/L; 5 milk samples) and obtained by the use of less specific polyclonal antibodies.…”
Section: Discussioncontrasting
confidence: 73%
“…fibroblasts, chondrocytes, lymphocytes, endothelial cells) is known to be trapped in insoluble multimeric fibrils, while that present in plasma (produced by hepatocytes) is released to the blood as a compact globular dimer [3,5]. The milk FN could originate from local synthesis by mammary epithelial cells, lymphocytes, and macrophages, and also part of it might originate from filtration of the blood [1]. FN mediates cellular interactions and plays important roles in cell adhesion, migration, proliferation, differentiation, tissue repair and extracellular matrix remodeling [5,6].…”
Section: Introductionmentioning
confidence: 97%
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“…1984), and another variety with low affinity to native type 1 collagen (Chandrasekhar et al 1983). Fibronectin has been identified in colostrum by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunological cross-reactivity (Steimer et al 1981;Zardi et al 1982), but it has not been detected in 80-d milk (Steimer et al 1981), nor has it been isolated from colostrum or milk. In this communication we report the purification of fibronectin from both milk and colostrum in order to investigate its structure and function in milk and the mammary gland, and we also compare its biochemical and biological properties with those of blood plasma fibronectin from the same species.…”
mentioning
confidence: 99%
“…The first is that fibronectin contained in hu man breast milk was absorbed in the gastroin testinal system and was reflected in the blood levels. Previous reports showed that mamma ry epithelial cells synthesize fibronectin [7] and that radioimmunoactivity of fibronectin was detected in human breast milk but not in commercial milk formulas [8]. The second is the difference in nutritional status of infants fed breast milk and those fed milk formulas.…”
Section: Discussionmentioning
confidence: 99%