Human monkeypox virus: Detection methods and perspectives for diagnostics

Stefano, Jéssica S.; Silva, Luiz; Kalinke, Cristiane; Oliveira, Paulo Roberto de; Crapnell, Robert D.; Brazaca, Laís Canniatti; Bonacin, Juliano Alves; Campuzano, Susana; Banks, Craig E.; Janegitz, Bruno C.

doi:10.1016/j.trac.2023.117226

Cited by 11 publications

(10 citation statements)

References 106 publications

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“…The fact that the developed immunosensor has fast response time is an important parameter in terms of practicality. 1 On the other hand, effective interaction between the analyte and receptor has an influence on the accuracy of the developed system. Therefore, the duration of interactions between the HS and A29 protein was optimized.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Monkeypox was used to define as an endemic African disease that causes not so much serious problems globally. Unfortunately, an increasing number of monkeypox cases have been reported in disparate regions of the world in early May 2022, and since it has been seen in more than 28 countries apart from African countries, recently it is declared as global public health emergency by the World Health Organization (WHO). ,, In this manner, it seems possible to have a monkeypox pandemic in the future considering the presence of asymptomatic human beings and the lack of massive MPXV testing . For this reason, rapid, effective, and economical detection tools for MPXV identifications are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

“…Until now, there are some monkeypox diagnostic methods that have been used for diagnosing the infections. Some of these techniques are polymerase chain reaction, , virus culture-based diagnosis, protein Cas-based diagnosis, , loop-mediated isothermal amplification, , enzyme-linked immunosorbent assay, , and recombinase polymerase amplification assay. ,, Considering the point-of-care (POC) nature of rapid tests, antibody-based immunoassays seem to be stronger diagnostic tool candidates for MPXV detection. However, due to the antigen context of Orthopoxvirus genomes, most human samples become cross-reactive, and this situation makes it impossible to differentiate different kinds of Orthopoxvirus viruses. − …”

Section: Introductionmentioning

confidence: 99%

“…Monkeypox can simply be described as a closely relevant pox disease in monkeys that is caused by the monkeypox virus (MPXV). MPXV is in the Poxviridae family and belongs to genus Orthopoxvirus . − Multiple neurological and physiological symptoms such as fatigue, headache, weakness, altered consciousness, nausea, and vomiting are reported in patients infected with this virus . Monkeypox was used to define as an endemic African disease that causes not so much serious problems globally.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Immunoassay Platform for Human Monkeypox Virus Detection

Can,

Perk,

Çitil

et al. 2024

Anal. Chem.

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In this study, we reported a selective impedimetric biosensor for the detection of A29 which is the target protein of the monkeypox virus (MPXV). The working principle of the biosensor relies on the interaction mechanism between A29, which is an internal membrane protein of MPXV, and the heparan sulfate receptor. For this purpose, after immobilizing heparan sulfate onto the gold screen-printed electrode surface, its interaction with A29 protein was monitored using electrochemical impedance spectroscopy. After the optimization of experimental parameters, the analytical characteristics of the developed MPVX immunosensor were examined. The developed immunosensor exhibited a linear detection range between 2.0 and 50 ng mL −1 , with a detection limit of 2.08 ng mL −1 and a quantification limit of 6.28 ng mL −1 . Furthermore, a relative standard deviation value of 2.82% was determined for 25 ng mL −1 . Apart from that, sample application studies were also performed with the standard addition of A29 protein to 1:10 diluted real serum samples that were taken from healthy individuals, and very good recovery values were obtained.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical Immunoassay Platform for Human Monkeypox Virus Detection

Can,

Perk,

Çitil

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the Orthopoxvirus genus ( Poxviridae family) and causative of monkeypox (Mpox) disease that presents symptoms similar to mild cases of smallpox. MPXV presents high human-to-human transmission, which may occur sexually and through large respiratory droplets, contact with skin lesions, or with infected bed and bath linen. − The first human case related to this disease was reported in 1970 in the Democratic Republic of Congo, and on July 23, 2022, it was formally recognized as a public health emergency of international concern (PHEIC) by World Health Organization (WHO) . Since then, around 87,000 cases were confirmed in more than 110 countries, which mainly included the USA (30,347 cases), Brazil (10,897 cases), and Spain (7549 cases). , …”

Section: Introductionmentioning

confidence: 99%